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Isolation and Characterization of Infectious Hematopoietic Necrosis Virus Causing High Mortality in Rainbow Trout Oncorhynchus mykiss  

Kim, Gi-Hong (Department of Aqualife Medicine. Yosu National University)
Kim, Yeong-Jin (Department of Aqualife Medicine. Yosu National University)
Jeong, Seong-Ju (Department of Aqualife Medicine. Yosu National University)
Jeong, Tae-Seong (Lab of Fish & Shellfish Diseases, KyungSang National University)
O, Myeong-Ju (Department of Aqualife Medicine. Yosu National University)
Publication Information
Journal of fish pathology / v.16, no.2, 2003 , pp. 81-90 More about this Journal
Abstract
High mortality with signs similar to viral haemorrhagic septicemia (VHS) such as severe haemorrhages in the skin, muscle and air bladder occurred in the farmed adult rainbow trout, Oncorhynchus mykiss, in Ku-mi and Je-chun area in Korea. The isolates were neutralized by an antiserum against IHNV but not by antisera against VHSV. Electron micrograph of an ultrathin section showed large numbers of bullet-shaped virus particles. The newly isolated rhabdovirus was composed of five structural proteins. In the western blot analysis Ihe anti-DiNV serum strongly reacted with G. N and MI protein. The cumulative mortalities of RTK infected rainbow trout (10-12cm.9-12g) with $10^{3.5}\;and\;10^{1.5}TCID_{50}/m{\ell}$ were 80% and 30%. respectiveIy_ RTJ infected fish showed 50% mortality by infection with $10^{3.5}TCID_{50}/m{\ell}$. Control group and IHNVChAb exhibited no mortality. From these results, the viruses were identified lHNV although diseased fish showed similar sign. with VHS and caused high mortality in large-sized fish.
Keywords
Rhabdovirus, IHNV, Rainbow trout
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