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Measurement of membrane fluidity of rockfish (Sebastes schlegeli) phagocytes during the respiratory burst using fluorescence polarization assay  

Jung, Jae-Hyuck
Kwon, Se-Ryun
Lee, Eun-Hye
Kim, Sung-Mi
Kim, Ki-Hong
Publication Information
Journal of fish pathology / v.16, no.2, 2003 , pp. 131-134 More about this Journal
Abstract
The change of membrane fluidity in rockfish (Sebastes schlegeli) phagocytes during respiratory burst was investigated. Fluorescence polarization (FP) was used as a measure of membrane fluidity, and 1-(4-trimethylaminophenyl)-6-phenyl-1 .3 ,5-hexatriene (TMA.-DPH) was used us a fluorescent probe. The significantly higher FP values in phagocytes stimulated With zymosan or phurbol myristate acetate (PMA) than unstimulated control phagocytes suggests that membrane fluidity of phagocytcs is decreased during the respiratory burst. The faster decrease of FP value in PMA stimulated phagocytes than in zymosan sumulated phagocytes may be due to bypass of the receptor-mediated stages of functional modulation. which is needed in zymosan stimulated phagocytes.
Keywords
Membrane fluidity; Phegccyre; Respiratory burst; Flcorescence polarization;
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