Browse > Article

Persistent Infection of Marine Birnavirus and its Status of Infection in Cells  

Jung, Sung-Ju (여수대학교 수산생명의학과)
Oh, Myung-Joo (여수대학교 수산생명의학과)
Publication Information
Journal of fish pathology / v.15, no.1, 2002 , pp. 9-16 More about this Journal
Abstract
The objective of the study was to clarify the mechanism of persistent infection of marine birnavirus (MABV) in various nonpermissive cell lines. It was observed in CHSE-214, RTG-2 and RSBK-2 that the virus produced at high yield with typical cytopathic effect (CPE). On the contrary, the CPE was not produced in EPC, FHM and BF-2 cells. However amount of virus protein in both permissive and nonpermissive cell lines detected by ELISA was almost the same. Electron microscopy showed virions in permissive cells but not in nonpermissive cells. From the results, it is clear that virus protein and RNA were produced in nonpermissive cells as observed in permissive cells; however, assembly of the virus particles did not occur in nonpermissive cells.
Keywords
Persistent infection; Marine birnavirus (MABV); Antigen expression; Virus replication;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hedrick, R.K. and Fryer, J.L.: Persistent infections of three salmonid cell lines with infectious pancreatic necrosis virus (IPNV). Fish Pathol., 15: 163-172, 1982
2 Suzuki, S., Kamakura, M. and Kusuda, R.: Isolation of birnavirus from Japanese pearl oyster Pinctada fucata. Fish. Sci., 64: 342-343, 1998   DOI
3 Dobos, P. and Roberts, T.E.: The molecular biology of infectious pancreatic necrosis virus: a review. Can. J. Microbiol., 29: 377-384, 1993
4 Nakajima, K., Maeno, Y., Arimoto, M., lnoue, K. and Sorimachi, M.: Viral deformity of yellowtail fingerlings. Fish Pathol., 28: 125-129, 1993   DOI
5 Heppell, J., Berthiaume, L., Corbin, F., Tarrab, E., Lecomte, J. and Arella, M. A.: Comparsion of amino acid sequences deduced from a cDNA fragment obtained from Infectious Pancreatic Necrosis Virus (IPNV) strains of different serotypes. Virol., 195: 840-844, 1993   DOI   ScienceOn
6 Hosono, N., Suzuki, S. and Kusuda, R.: Genogrouping of birnaviruses isolated from marine fish : a comparison of VP2/NS junction regions on genome segment A. J. Fish Dis., 19: 298-302, 1996
7 Jung, S. J.: Change of infection properties of subcultured marine birnavirus in several fish cell lines. J. Fish Pathol., 11: 89-96, 1998
8 Suzuki, S., Nakata, T., Kamakura, M., Yoshimoto, M., Furukawa, Y., Yamasita, Y. and Kusuda, R.: Isolation of birnavirus from agemaki (jack knife clam) Sionovacura conatricta and survey of the virus using PCR technique. Fish. Sci., 63: 563-566, 1997
9 Read, S. M. and Northcote, H.: Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal. Biochem., 116: 53-64, 1981   DOI   ScienceOn
10 Kusuda, R., Nishi, Y., Hosono, N. and Suzuki, S.: Serological comparison of birnaviruses isolated from several species of marine fish in south west Japan. Fish Pathol., 28: 91-92, 1993   DOI
11 Chou, H-Y., Li, H-J. and Lo, C-F.: Pathogenicity of a birnavirus to hard clam (Meretrix lusoria) and effect of temperature stress on its virulence. Fish Pathol., 29: 171-175, 1994   DOI
12 Takeura, S., Aoki, H., Tsurumi, T., Nishiyama, Y., Fujioka, H., Yosii, S. and Maeno, Y.: Abortive infection of L cells by influenza B virus: defect in bud formation. Microbiol. lmmunol., 28: 427-437, 1984   DOI
13 Duncan, R., Nagy, E., Krell, P.J. and Dobos, P.: Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of virus-encoded protease, and fine structure mapping of genome segment A coding regions. J. Virol., 61: 3655-3664, 1987
14 Rivas, C., Cepeda, C., Dopazo, C.P., Novoa, B., Noya, M. and Barja, J.L.: Marine environment as reservoir of birnaviruses from poikilothermic animals. Aquaculture, 115: 183-194, 1993   DOI
15 Sorimachi, M. and Hara, T.: Characteristics and pathogenicity of a virus isolated from Yellowtail fingerlings showing ascites. Fish Pathol., 19: 231-238, 1985   DOI