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http://dx.doi.org/10.12925/jkocs.2021.38.5.1335

Biosynthesis of Compound K, a biologically active saponin of ginseng(Panax ginseng) by bioconversion  

Kim, MooSung (Macrocare Co., Ltd)
Kim, Ja-i (Division of Food Science and Biotechnology, Korea National University of Transportation)
Jung, Kyung-Hwan (Division of Food Science and Biotechnology, Korea National University of Transportation)
Yu, Kwang-Won (Division of Food Science and Biotechnology, Korea National University of Transportation)
Moon, Gi-Seong (Division of Food Science and Biotechnology, Korea National University of Transportation)
Lee, Hyang-Yeol (Division of Food Science and Biotechnology, Korea National University of Transportation)
Publication Information
Journal of the Korean Applied Science and Technology / v.38, no.5, 2021 , pp. 1335-1344 More about this Journal
Abstract
Ginsenoside Compound K is a triterpene saponin found in the leafs, stems and roots of Panax ginseng. This study aimed to prepare a valuable ginsenoside Compound K using ginseng extracts with the enzyme(Plantase). Plantase showed very efficient activity to produce Compound K from ginseng extracts. Plantase exhibited the highest activity at pH 5 and 50 ℃, as a result of investigating the yield of Compound K by changing the temperature and pH, while fixing the enzyme concentration to 10% or 15% over 48 hours of reaction time. Under optimium conditions, Plantase produced and accumulated Compound K over 35 wt% of whole ginseng extracts. Antimicrobial activitiy of bioconvertied ginseng extracts showed selectivity against Cutibacterium acnes KCTC 3314. Minimal inhibitory concentration (MIC) of bioconverted ginseng extract (35% of Compound K enriched extract) against Cutibacterium acnes KCTC 3314 strain is 31.25ug/mL. These results suggest that the Compound K enriched extract is potential materials for cosmetic products and Plantase is a very useful enzyme for Compound K production.
Keywords
Ginsenoside; Compound K; Bioconversion; Panax ginseng; Antimicrobial effect;
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Times Cited By KSCI : 1  (Citation Analysis)
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