Browse > Article
http://dx.doi.org/10.12925/jkocs.2021.38.5.1302

Comparative Study of the Antioxidant Activity Effects of Gami-Sumiwon Extract using Water and 80% Ethanol  

Lim, Hyun-Ji (Department of Beauty, Daeduck University)
Publication Information
Journal of the Korean Applied Science and Technology / v.38, no.5, 2021 , pp. 1302-1313 More about this Journal
Abstract
In this study, Gami-Sumiwon (GS; Cynanchi wilfordii R., Angelica gigantis R., Lycium chinense M., Betula platyphylla S., Cryptotympana atrata F. and Carthamus tinctorius L.) and/or Sinomenium acutum R. (SC) was extracted with 70% ethanol or water. And We investigated the antioxidant activity effect of GS±SC. The following experimental techniques were used to evaluate the antioxidant efficacy of GS±SC. HPLC chromatogram, heavy metal content, ABTS/DPPH radical scavenging analysis, SOD-like activity assay, FACS, and NO assay. As a result of the experiment, the sinomenine content was found to be higher in DW extracts, and decursin was found to be higher in 80% ethanol extracts. And, the amount of heavy metals in all extracts was below the standard value. ABTS, and DPPH radical scavenging activity was identified that GS±SC(EtOH) was found to have a higher scavenging activity than GS±SC(DW). But, SOD showed the opposite result. No cytotoxicity of GS was observed on Raw 264.7 cells at concentration of 1~100 ㎍/㎖. The ROS production was significantly decreased that GS±SC(DW) was found to have a higher scavenging activity than GS±SC(EtOH). However, NO production showed the opposite result. Looking at the results of SOD and ROS analysis, SC does not seem to have a function of prevention. SC is thought to have an effect on the removal of free radicals generated after oxidative stress. This result objectively confirmed the antioxidant effect of GS±SCs. We will continue to conduct in-depth research. Therefore, it is believed that the possibility of using GS±SCs as a functional material can be established. The more diverse the objectives, the higher the value of GS utilization is thought to be.
Keywords
Gami-Sumiwon; Sinomenium acutum R; antioxidant; oxidative stress; functional material;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 D. H. Shin, J. S. Han, M. S. Kim, "Antimicrobial Effect of Ethanol Extracts of Sinomenium acutum(Thunb.) Rehd. et Wils and Glycyrrhiza glabra L. var. Glandulifera Regel et Zucc on Listeria monocytogenes", Korean Journal Food Science Technology, Vol.26, No.5 pp.627- 632, (1994).
2 S. S. Lee, H. J. Lee, D. H. Choi, "StudiesStudies on Biological Activity of Wood Extractives(VII) - Antimicrobial and Antioxidation Activities of Extractives from the Heartwood of Prunus sargentii", Journal of the Korean Wood Science and Technology, Vol.9, No.4 pp.140-145, (2001).
3 B. H. Han, Y. N. Han, "Immunosuppressant Activity of Cherry Bark Extract", Korean Journal of Pharmacognosy, Vol.29, No.2 pp.173-175, (1978).
4 S. Salvati, L. M. Campeggi, P. C. Benedetti, M. D. Felice, V. Gentile, M. Nardini, G. Tomassi, "Effects of dietary oils on fatty acid composition and lipid peroxidation of brain membranes (myelin and synaptosomes) in rats", The Journal of Nutritional Biochemistry, Vol.4, No.6 pp.346-350, (1993).   DOI
5 B. J. An, Y. J. Cho, J. H. Son, J. M. Park, J. Y. Lee, T. S. Park, "Antioxidant Effects and Tyrosinase Inhibition Activity of Extract of Prunus sargentii Rehder", Journal Applied Biological Chemistry, Vol.49, No.2 pp.145-148, (2006).
6 O. H. Lee, S. Y. Yu, Y. J. Lee, H. S. Song, H. D. Hong, J. H. Lim, H. S. Choi, B. Y. Lee, S. N. Kang, "Antioxidant Effects and Nitrite Scavenging Ability of Extract from Acanthopanax cortex Shoot", The Korean Journal of Food And Nutrition, Vol.25, No.4 pp.793-799, (2012).   DOI
7 H. S. Choi, M. F. Zhu, C. S. Kim, "Studies of Name and Herbal Origins of Ha-Soo-Oh", Korean Journal of Oriental Medicine, Vol.9, No.1 pp.81-89, (2003).
8 J. Kim, "Comparison of antioxidant activities of extracts of Limonium tetragonum, Gleichenia japonica, Sinomenium acutum leaves with different solvents", Journal of Advanced Engineering and Technology, Vol.14, No.1 pp.35-42, (2021).   DOI
9 H. Y. Ryu, J. C. Heo, J. S. Hwang, S. W. Kang, C. Y. Yun, S. H. Lee, H. Y. Sohn, "Screening of Thrombin Inhibitor and its DPPH Radical Scavenging Activity from Wild Insects", Journal of Life Science, Vol.18, No.3 pp.363-368, (2008).   DOI
10 J. H. Song, S. G. Yang, W. J. Kim, B. C. Moon, "Studies on morphological characteristics of the original species, Cryptotympana atrata as a Cicadidae Periostracum", Korean Herbal Medicine Informatics, Vol.7 No.1 pp.13-23, (2019).   DOI
11 W. Fiers, R. Beyaert, W. Declercq, P. Vandenabeele, "More than one way to die: apoptosis, necrosis and reactive oxygen damage", Oncogene, Vol.18, No.54 pp.7719-7730, (1999).   DOI
12 Z. Zhao, J. Xiao, J. Wang, W. Dong, Z. Peng, D. An, "Anti-inflammatory effects of novel sinomenine derivatives", International Immunopharmacology, Vol.29, No.2 pp.1385-1390, (2015).
13 K. W. Park, S. R. Choi, M. Y. Shon, I. Y. Jeong, K. S. Kang, S. T. Lee, K. H. Shim, K. I. Seo, "Cytotoxic Effects of Decursin from Angelica gigas Nakai in Human Cancer Cells", Journal of the Korean Society of Food Science and Nutrition, Vol.36, No.11 pp.1385-1390, (2007).   DOI
14 S. H. Lee, S. H. Lee, M. L. Jin, C. O. Hong, M. Hur, J. W. Han, W. M. Lee, H. M. Yun, Y. B. Kim, Y. Lee, S. C. Koo, "Analysis of Index Component Content and Antioxidant Activity According to the Root Diameter of Angelica gigas Nakai", Korean journal of plant resources, Vol.32, No.2 pp.116-123, (2019).   DOI
15 J. J. V. Triel, J. H. E. Arts, H. Muijser, C. F. Kuper, "Allergic inflammation in the upper respiratory tract of the rat upon repeated inhalation exposure to the contact allergen dinitrochlorobenzene (DNCB)", Toxicology, Vol.269, No.1 pp.73-80, (2010).   DOI
16 Chipiti T, Ibrahim MA, Koorbanally NA, Islam S. "In vitro antioxidant activities of leaf and root extracts of Albizia antunesiana Harms", Polish Pharmaceutical Society, Vol.70, No.6 pp.1035-1043, (2013).
17 M. Guha, N. Mackman, "LPS induction of gene expression in human monocytes". Cellular Signalling, Vol.13, No.2 pp.85-94. (2001).   DOI
18 H. J. Lee, H. J. Lim, M. H. Lim, "The Activity of Anti-oxidation of Cinnamomum loureiroi Extract", Journal of the Korean Applied Science and Technology, Vol.37, No.6 pp.1583-1590, (2020).   DOI
19 H. J. Lee, H. J. Lim, M. H. Lim, "Effect of Aucklandia lappa Decne Extract on Antioxidant", Journal of the Korean Applied Science and Technology, Vol.37, No.6 pp.1545-1555, (2020).   DOI
20 S. H. Yoon, J. W. Kim, "Antioxidative Effects of various Antioxidants on the Soybean Oil", Journal of the Korean Society of Food Science and Nutrition, Vol.17, No.1 pp.19-23, (1988).
21 H. S. Kim, "Effects of Cynanchum wilfordii Extract on Serum Lipid Components and Enzyme Activities in Hyperlipidemic and Streptozotocin-Induced Diabetic Rats", Korean Journal of Human Ecology, Vol.7, No.2 pp.1-11, (2004).
22 B. H. Park, H. S. Cho, D. H. Kim, "Antioxidative Effects of Solvent Extracts of Lycii fructus Powder (LFP) and Maejakgwa Made with LFP", The Korean Society of Food Science and Nutrition, Vol.34, No.9 pp.1314-1319, (2005).   DOI
23 S. O. Lee, H. J. Lee, M. H. Yu, H. G. Im, I. S. Lee, "Total Polyphenol Contents and Antioxidant Activities of Methanol Extracts from Vegetables produced in Ullung Island", Korean Society of Food Science and Technology, Vol.37, No.2 pp.233-240 (2005).
24 H. J. Lim, H. J. Lee, M. H. Lim, "Comparison of Antioxidant and Anti-inflammatory Activity of Korean Houttuynia cordata Thunb. Extracts", Journal of the Korean Applied Science and Technology, Vol.38, No.1 pp.217-227, (2021).   DOI
25 S. J. Lee, E. J. Lee, W. D. Park, J. B. Kim, S. W. Choi, "Antioxidant and antiinflammatory activities of extracts from Korean traditional medicinal prescriptions", Korean Journal of Food Science and Technology, Vol.43, No.5 pp. 624-632, (2011).   DOI
26 S. H. You, J. S. Moon, "A study on anti-oxidative, anti-inflammatory, and melanin inhibitory effects of Chrysanthemum sibiricum extract", The Korean Society of Applied Science and Technology, Vol.33, No.4 pp.762-770, (2016).
27 H. J. Lim, J. K. Kim, K. M. Cho, "Analysis of nutritional components, volatile properties, and sensory attributes of Cynanchi wilfordii Radix: characterization Study", Journal of the Korean Society of Food Science and Nutrition, Vol.44, No.4 pp.564-572, (2015).   DOI
28 D. U. Lee, W. C. Lee, "An 5-Lipoxygenase Inhibitor Isolated from the Roots of Cynanchum wilfordi Hemsley", Natural Product Sciences, Vol.28, No.4 pp.247-251, (1997).
29 J. W. Choi, H. S. Lee, Y. E. Kim, B. M. Kim, I. H. Kim, C. H. Lee, "Effect of Cynanchi wilfordii Radix extracts on lipid compositions and blood pressure in spontaneously hypertensive rats", Journal of the Korean Society of Food Science and Nutrition, Vol.41, No.3 pp.345-350, (2012).   DOI
30 J. H. Park, Y. J. Lee, S. J. Keon, "Pharmacognostical Studies on the Dang Gui from Korea", Natural Product Sciences, Vol.36, No.2 pp.141-144, (2005).
31 N. J. Cho, W. H. Lee, K. K. Kim, H. S. Han, "Investigation of the antioxidant effect of Angelicae Radix from Korea, China and Japan", Journal of Physiology & Pathology in Korean Medicine, Vol.31, No.3 pp.182-187, (2017).   DOI
32 H. K. Kim, G. M. Na, S. H. Ye, H. S. Han, "Extraction Characteristics and Antioxidative Activity of Lycium chinense Extracts", Korean Journal of Food Preservation, Vol.11, No.3 pp.352-357, (2004).
33 C. Deby, R. Goutier, "New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase", Biochemical Pharmacology, Vol.39, No.3 pp.399-405, (1990).   DOI
34 N. Dastmalchi, B. Baradaran, S. LatifiNavid, R. Safaralizadeh, S. M. B. Khojasteh, M. Amini, E. Roshani, and P. Lotfinejad, "Antioxidants with Two Faces Toward Cancer", Life Sciences, Vol.258, No.1 pp.118-186, (2020).
35 Z. G. Zhao, L. M. Zhang, W. Song, H. B. Du, H. Cui, C. Y. Niu, "Normal mesenteric lymph ameliorates acute kidney injury following lipopolysaccharide challenge in mice", Renal Failure, Vol.36, No.8 pp.1304-1309, (2014).   DOI
36 Y. J. Chang, D. L. Jung, S. U. Hong, "The effect of Periostracum Cicadae on capsaicin-induced model of atopic dermatitis in rats", Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology, Vol.28, No.4 pp.41-50, (2015).   DOI
37 S. A. Kim, H. K. Oh, J. Y. Kim, J. W. Hong, S. I. Cho, "A review of pharmacological effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their bioactive compounds", Journal of Korean Medicine, Vol.32, No.4 pp.1-24, (2011).
38 J. W. Lee, J. B. Weon, B. R. Yun, M. R. Eom, C. J. Ma, "Quantitative Analysis for the Quality Evaluation of Sinomenine, Magnoflorine and Syringaresinol in Sinomenium acutum", The Pharmaceutical Society Of Korea, Vol.57, No.3 pp.161-166, (2013).
39 K. S. Han, G. J. Shin, W. C. Lee, "Study on the Antioxidative Effects and Amino Acid Contents of the Roots of Cynanchum Wilfori", Journal of Internal Korean Medicine, Vol.19, No.2 pp.411-430, (1998).
40 S. S. Ghee, Y. J. Lee, "The therapeutic effects of Bangkeehwangkee-tang and Bangkeebokryeong-tang on the hyperlipidemia in rats", Korea Journal of Herbology, Vol.20, No.2 pp.149-157, (2005).
41 L. I. Choi, Y. M. Lee, T. R. Heo, "Screening of Hyaluronidase Inhibitory and Free Radical Scavenging Activity in vitro of Traditional Herbal Medicine Extracts", Korean Journal of Biotechnology and Bioengineering, Vol.18, No.4 pp.282-288, (2005).
42 H. K. Kim, Y. E. Kim, J. R. Do, Y. C. Lee, B. Y. Lee, "Antioxidative activity and physiological activity of some korean medicinal plants", Korean Society of Food Science and Technology, Vol.27, No.1 pp.80-85, (1995).
43 K. S. Oh, Y. Yang, "Effects of Gujija Extract Supplementation and Swimming Exercise on Body Weight, Fat Mass, Lipid Metabolism and Glycometabolism in High Fat-diet Mice", Journal of Wellness, Vol.8, No.1 pp.129-137, (2013).