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http://dx.doi.org/10.12925/jkocs.2021.38.3.801

Skin Absorption and Physical Property of Ceramide-added Ethosome  

Hyeon, Tong-Il (Department of Chemistry & Cosmetics, Jeju National University)
Yoon, Kyung-Sup (Department of Chemistry & Cosmetics, Jeju National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.38, no.3, 2021 , pp. 801-812 More about this Journal
Abstract
In order to delivery biotin to skin, ethosomes containing both biotin and ceramide were researched by using high pressure homogenizer. Biotin was utilized as a drug and ceramide NP was utilized as a supporter of bilayer. The biotin was entrapped in aqueous core, while ceramide NP was packed in the bilayer of the ethosomes. Looking at the physical properties of vesicles containing ceramide NP, the sized was 80~130 nm, the polydispersity index was 0.09~0.16, and the zeta potential was -40~-49 mV. In vesicles without ceramide NP, the size was 124.80±1.46 nm, and the zeta potential and polydispersity index were -45.48±1.27 mV and 0.088±0.018, respectively. Therefore, the ethosome with ceramide NP has improved physical properties of vesicles compared to the ethosome without ceramide NP. Skin absorption rates of ethosomes with ceramide NP were 6.13~14.98%, while skin absorption rate of ethosome without ceramide NP was 7.08% at 12 h. In conclusion, ethosomes containing ceramide NP not only improved the skin absorption efficiency, but had also a positive effect on the stability of vesicles.
Keywords
Ethosome; Ceramide NP; High pressure homogenizer; Zeta potential; Skin absorption rate;
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  • Reference
1 R. M. Trueb, "Serum biotin levels in women complaining of hair loss", International Journal of Trichology, Vol.8, No.2, pp. 73-77, (2016).   DOI
2 A. Overbye, A. Holsaeter, M. Fusser, N. Skalko-Basnet, T. Inversen, M. Lyngass Torgersen, T. Sonstevold, O. Engebarraten, K. Flatmark, G. M. Maelandsmo, T. Skotland, K. Sandvig, "Ceramide-containing liposomes with doxorubicin: time and cell-dependent effect of C6 and C12 ceramide", Oncotarget, Vol.8, No.44, pp. 76921-76934, (2017).   DOI
3 S. Kaddaha, N. Khreich, F. Kaddah, C. Charcosset, H. Greige-Gerges, "Cholesterol modulates the liposome membrane fluidity and permeability for a hydrophilic molecule", Food and Chemical Toxicology, Vol.113, pp. 40-48, (2018).   DOI
4 J. M. Lopez-Pinto, M. L. GonzalezRodriguez, A. M. Rabasco, "Effect of cholesterol and ethanol on dermal delivery from DPPC liposomes", International Journal of Pharmaceutics, Vol.298, No.1, pp. 1-12, (2005).   DOI
5 R. B. Rodriguez, M. Sabes, "Factors involved in the production of liposomes with a high-pressure homogenizer", International Journal of Pharmaceutics, Vol.213, No.1-2, pp. 175-186, (2001).   DOI
6 K. Y. Chae, S. S. Kwon, S. N. Park, "A study on Nano-emulsion for enhanced transdermal delivery of Hippophae rhamnoides leaf extract", Applied Chemistry for Engineering, Vol.24, No.3, pp. 260-265, (2013).
7 H. J. Gwak, B. S. Jin, "Preparation and characterization of EGCG entrapped ethosome", The Korean Society of Industrial and Engineering Chemistry, Vol.18, No.2, pp. 130-135, (2007).
8 E. Touitou, N. Dayan, L. Bergelson, B. Godin, M. Eliaz, "Ethosome-novel vesicular carriers for enhaced delivery: Characterization and skin penetration properties", Journal of Controlled Release, Vol.65, No.3, pp. 403-418, (2000).   DOI
9 A. Laouini, C. J. Maalej, I. L. Blouza, S. Sfar, C. Charcosset, H. Fessi, "Preparation, characterization and applications of liposomes: State of the art", Journal of Colloid Science and Biotechnology, Vol.1, No.2, pp. 147-168, (2012).   DOI
10 J. S. Dua, A. C. Rana, A. K. Bhandari, "Liposome: Methods of preparation and applications", International Journal of Phamaceutical Sciences and Research, Vol.3, No.2, pp. 14-20, (2012).
11 L. Cui, Y. Jia, Z. W. Cheng, Y. Gao, G. L. Zhang, J. Y. Li, C. F. He, "Advancements in the maintenance of skin barrier/skin lipid composition and the involvement of metabolic enzymes", Journal of Cosmetic Dermatology, Vol.15, No.4, pp. 549-558, (2016).   DOI
12 M. Rabionet, K. Gorgas, R. Sandhoff, "Ceramide synthesis in the epidermis", Biochimica et Biophysica Acta, Vol.1841, No.3, pp. 422-434, (2014).   DOI
13 A. Sugiura, T. Nomura, A. Mizuno, G. Imokawa, "Reevaluation of the nonlesional dry skin in atopic dermatitis by acute barrier disruption: an abnormal permeability barrier homeostasis with defective processing to generate ceramide", Archieves of Dermatological Research, Vol.306, No.5, pp. 427-440, (2014).   DOI
14 S. J. Yang, K.-S. Yoon, "Study of the stability of biotin encapsulated by nano liposome", Journal of Society Cosmetic Science Korea, Vol.46, No.2, pp. 133-145, (2019).
15 M. Brandl, D. Bachmann, M. Drechsler, K. H. Bauer, "Liposome preparation by a new high pressure homogenizer Gaulin Micron Lab 40", Drug Development and Industrial Pharmacy, Vol.16, No.14, pp. 2167-2191, (1990).   DOI
16 G Gregoriadis, Liposome Technology 2 nd Edition, pp. 50-65, CRC press, (1993).
17 M. N. Trainer, P. J. Freud, E. M. Leonardo, "High-concentration submicron particle size distribution by dynamic light scattering", American Laboratory, Vol.24, No.11, pp. 34-39, (1992).
18 N. Ostrowsky, "Liposome size measurements by photon correlation spectroscopy", Chemistry and Physics of Lipids, Vol.64, No.1-3, pp. 45-56, (1993).   DOI
19 Z. Zhang, Y. Wo, Y. Zhang, D. Wang, R. He, H. Chen, D. Cui, "In vitro study of ethosome penetration in human skin and hypertrophic scar tissue", Nanomedicine, Vol.8, No.6,, pp. 1026-1033, (2012).   DOI
20 C. K. Kim, M. H. Min, K. H. Min, W. R. Lah, B. J. Lee, Y. B. Kim, "Synthesis of n-stearyl actobionamide (N-SLBA) and preparation of neo-galactosylated liposome", Yakhak Hoeji, Vol.36, No.2, pp. 159-166, (1992).
21 G. M. El Maghraby, B. W. Barry, A. C. Williams, "Liposomes and skin: From drug delivery to model membranes", European Journal of Phamaceutical Sciences, Vol.34, No.4-5, pp. 203-222, (2008).   DOI
22 M. A. Bolzinger, S. Briancon, J. Pelletier, Y. Chevalier, "Penetration of drugs through skin, a complex rate-controlling membrane", Current Opinion in Colloid & Interface Science, Vol.17, No.3, pp. 156-165, (2012).   DOI
23 H. Jeong, J. Yoo, H. S. Lee, J. H. Seo, W. B. Ko, "Nanotechnology application in cosmetics", KIC News, Vol.12, No.1, (2009).
24 D. D. Verma, S. Verma, G. Blume, A. Fahr, "Particle size of liposomes influences dermal delivery of substances into skin", International Journal of Pharmaceutics, Vol.258, No.1-2, pp. 141-151, (2003).   DOI