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http://dx.doi.org/10.12925/jkocs.2021.38.1.99

Optimal Conditions for Phenylethanol Galactoside Synthesis using Escherichia coli β-Galactosidase  

Jung, Kyung-Hwan (Major in Biotechnology, Korea National University of Transportation)
Publication Information
Journal of the Korean Applied Science and Technology / v.38, no.1, 2021 , pp. 99-106 More about this Journal
Abstract
To circumvent the skin problem from phenylethanol (PhE), we have studied on the enzymatic synthesis of phenylethanol galactoside (PhE-gal) as an alternative to PhE. Base on the previous study, we optimized the reaction conditions for PhE-gal synthesis from PhE using E. coli β-galactosidase (β-gal). The optimal amount of β-gal, PhE concentration, pH, and temperature for PhE-gal synthesis were 0.45 U/ml, 1%, 8.0, 40℃, respectively. Under these conditions, about 81.9 mM PhE was converted into about 47.4 mM PhE-gal, in which the conversion yield was about 57.9%. Meanwhile, when the reaction mixture containing PhE and PhE-gal was mixed and fractionated with water-immiscible solvent (EA or MC), it was observed that PhE-gal was distributed in water phase, and PhE was distributed in solvent phase. Additionally, PhE-gal was clearly distributed into water phase when MC was used, but PE-gal was not when EA was used. In the future, we are planning to carried out the continuing study on developing an alternative cosmetic preservative using PhE-gal.
Keywords
Phenylethanol galactoside; ${\beta}-Galactosidase$; Transgalactosylation; Optimization; Solvent fractionation;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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