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http://dx.doi.org/10.12925/jkocs.2020.37.4.820

Collagen synthesis ability and inhibitory effect of MMPs in keratinocytes of Lysimachia christinae Hance Extract  

Kim, Ju-Eun (Department of Cosmeceutical Science, Daegu Haany University)
Choi, Yun-Sik (College of Pharmacy, Kyungsung University)
Kim, Hye Kyung (College of Pharmacy, Kyungsung University)
Jang, Young-Ah (Convergence Research Center for Smart Healthcare, R&DB Foundation of Kyungsung University)
Publication Information
Journal of the Korean Applied Science and Technology / v.37, no.4, 2020 , pp. 820-829 More about this Journal
Abstract
This study was conducted to investigate the anti-oxydant and anti-winkle efficacy as cosmetics ingredient of Lysimachia christinae Hance. Recently, the study of wrinkle improvement of natural products has received continuous interest. so we looked at relationship between reactive oxygen species (ROS) generation and pro-collagen synthesis and matrix metalloproteinases (MMPs) through this study. L. christinae Hance were extracted with 70% ethanol (LcHE) and distilled water (LcHW), respectively, and the experiment was conducted. LcHE had better ROS inhibition effect than LcHW and showed no toxicity up to 250 ㎍/mL concentration as a result of MTT assay in HaCaT cells, so we selected LcHE and conducted the wrinkle improvement material study. We confirmed that the synthesis of type-1 pro-collagen reduced by UVB is activated through pro-collagen synthesis assay. we confirmed that LcHE inhibited the increase in MMP-1 -3 -9 of MMPs induced by UVB in skin cells through western blot and we also performed real-time PCR to confirm the effect of the extract with dependence of concentration at mRNA levels. Therefore, it is expected that Lysimachia christinae Hance is used as a natural material for cosmetics that can effectively prevent wrinkles and skin aging by UVB.
Keywords
Anti-aging; Lysimachia christinae Hance; matrix metalloproteinases (MMPs); pro-collagen; Reactive Oxygen Species (ROS);
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