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http://dx.doi.org/10.12925/jkocs.2020.37.1.124

Comparative Study of the Biological Activity Effects of Manjakani (Quercus infectoria Olivier) Extract using Water and 80% Ethanol  

Lee, Hea-Jin (Department of Beauty Healthcare, Daejeon University)
Kim, Dong-Han (Joonae Coporation)
Lee, Eun-Jin (Matieu Co. Ltd.)
Lim, Mi-Hye (Department of Beauty Healthcare, Daejeon University)
Publication Information
Journal of the Korean Applied Science and Technology / v.37, no.1, 2020 , pp. 124-132 More about this Journal
Abstract
The purpose of this study was to investigate the biological activities such as cytotoxicity and anti-inflammation using Manjakani (Quercus infectoria Olivier) extract. Manjakani was extracted from hot DW and 80% ethanol. Cell viability was assessed using MTT assay on RAW 264.7 cells. Also, anti-inflammatory activities were measured through changes in the levels of nitric oxide (NO), prostaglandin E2 (PGE2), leukotrien B4 (LTB4), pro-inflammatory cytokines (IL-1β, IL-6 and tumor necrosis factor (TNF)-α) and transcription factor on LPS-induced RAW264.7 cells. The results confirmed that significant cytotoxicity does not appear in the concentration range of 1, 5, and 10 ㎍/㎖ of both extracts of this study. The production of NO was slowed by approximately MDE 37.2% and MEE 43.7% at 10 ㎍/㎖ concentration. Also, level of PGE2 and LTB4 was decreased MDE 30.9%/MEE 43.7% and MDE 37.1%/MEE 43.7%. In the case of inflammatory cytokine was reduced to MDE 38.8%/MEE 50.8% for IL-1β, MDE 35.0%/MEE 44.2% for IL-6 and MDE 31.9%/MEE 36.6% for TNF-α at 10 ㎍/㎖ concentration. The mRNA expression of NF-κB, iNOS and COX-2 significantly decreased by MDE 44.0%/MEE 16.0%, MDE 44.0%/MEE 55.0% and MDE 45.0%/MEE 40.0%, respectively, following the 10 ㎍/mL sample treatment when compared to the control. Both extracts were effective in anti-inflammatory activity. In addition, both extracts showed efficient changes of production of NO, PGE2, LTB4, pro-inflammatory cytokines and transcription factor. But MEE was found to have a higher inhibitory effect than MDE. In other words, Manjakani was showed significant biological activities showing anti-inflammation without cytotoxicity. These results will be provided as fundamental data for further development of the new health food and therapeutics related to the results above.
Keywords
Manjakani; cytotoxicity; anti-inflammation; biological activities; raw material;
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1 G. Kaplanski, V. Marin, F. Montero-Julian, A. Mantovani, C. Farnarier, "IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation", Trends Immunol., Vol.24, No.1 pp. 25-29, (2003).   DOI
2 M. A. O'Connell, B. L. Bennett, F. Mercurio, A. M. Manning, N. Mackman, "Role of IKK1 and IKK2 in lipopolysaccharide signaling in human monocytic cells", J. Biol. Chem., Vol.273, No.46 pp 30410-30414, (1998).   DOI
3 B. Hinz, K. Brune, "Cyclooxygenase-2-10 years later" J. Pharmacol. Exp. Ther., Vol.300, No.2 pp 367-375, (2002).   DOI
4 K. A. Kim, H. S. YI, H. J. YUN, S. D. Park, "Anti-oxidative and Anti-inflammatory Effect of Fractionated Extracts of Cynomorium Songaricum", Kor. J. Orient. Physiol. Pathol., Vol.23, No.6 pp 1320-1331, (2009).
5 F. B. Dayang, S. T. Liy, S. Zaleha, M. Z. Noraziah, "In Vitro Antibacterial Activity of Galls of Quercus infectoria Olivier against Oral Pathogens", Evid.-Based Complementary Altern. Med., pp 1-6 (2012).
6 Muhammad Z, Mustafa AM. Chapter 6. Kuala Lumpur (MY): Penerbit Fajar Bakti Sdn Bhd. p. 29-32, Traditional Malay Medicine Plants, (2010).
7 G. Kaur, M. Athar, M. S. Alam, "Quercus infectoria gall posses antioxidant activity and abrogates oxidative stress-induced functional alterations in murine macrophages", Chem. Biol. Interact., Vol.171, No.3 pp 272-282, (2008).   DOI
8 D. F. Basri, S. H. Fan, "The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents", Indian J. Pharmacol., Vol.37, No.1 pp 26-29, (2005).   DOI
9 S. P. Umachigi, K. N. Jayaveera, C. K. A. Kumar, G. S. Kumar, B. M. V. Swamy, D. V. K. Kumar, "Studies on wound healing properties of Quercus infectoria", Trop. J. Pharm. Res., Vol.7, pp 913-919, (2008).
10 H. J. Lee, B. Y. Sim, J. W. Bak, D. H. Kim, "Effect of Gami-sopungsan on Inflammation and DNCB-induced Dermatitis in NC/Nga in Mice", J. Physiol. & Pathol. Korean Med., Vol.28, No.2 pp 146-153, (2014).
11 H. O. Hamad, M. H. Alma, I. Gulcin, M. A. Yilmaz, E. Karaogul, "Evaluation of Phenolic Contents and Bioactivity of Root and Nutgall Extracts from Iraqian Quercus infectoria Olivier". Rec. Nat. Prod., Vol.11, No.2 pp 205-210, (2017).
12 K. S. Kang, H. J. Lee, B. Y. Sim, J. W. Bak, H. J. Choi, D. H. Kim, "Anti-inflammatory Effect of Cornus officinalis Fruit Extract for Potential of Cosmetic Ingredient", Kor. J. Aesthet. Cosmetol., Vol. 13, No.4 pp 461-468, (2015).
13 S. R. Hwang, H. J. Lee, B. Y. Sim, "The Anti-inflammatory Effects of Ethanol Extract in Euphorbia Supina Rafinesque", J. Kor. Soc. Cosm., Vol.20, No.6 pp 976-981, (2014).
14 H. J. Lee, M. H. Lim, "Antioxidation Effect of Lemongrass and Maychang Essential Oil", J. Kor. Soc. Cosm., Vol.22, No.2 pp 319-326, (2016).
15 C. Gabay, "Interleukin-6 and chronic inflammation", Arthrit. Res. Ther., Vol.8, No.2 pp 1-3, (2016).
16 T. J. J. Van, J. H. Arts, J. H. Muijser, C. F. Kuper, "Allergic inflammation in the upper respiratory tract of the rat upon repeated inhalation exposure to the contact allergen dinitrochlorobenzene (DNCB)", Toxicology, Vol.269, No.1 pp 73-80, (2010).   DOI
17 J. W. Bak, B. Y. Sim, H. J. Lee, D. H. Kim, "Effect of Cheongungdajosangamibang on oxidative damage and inflammation", Kor. J. Herbology, Vol.29, No.4 pp 53-59, (2014).   DOI
18 Y. J. Lee, B. Y. Sim, H. J. Lee, J. W. Bak, D. H. Kim, "Effect of Gami-Chunggisan on Antioxidant and Pro-Inflammatory Cytokine" Kor. J. Herbology, Vol.29, No.4 pp 69-76, (2014).   DOI