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http://dx.doi.org/10.12925/jkocs.2019.36.4.1259

Anti-inflammatory and Anti-oxidative Activities of Flavonoids Extracted from Dendranthema indicum Flowers in Jeju Island  

Hyun, Ju Mi (R&D Team, Jeju Industry-University Convergence Center)
Jo, Yeon Jeong (R&D Team, Jeju Industry-University Convergence Center)
Kim, Yun Beom (NewMedion Co., Ltd)
Park, Sung-Min (NewMedion Co., Ltd)
Yoon, Kyung-Sup (Department of Chemistry and Cosmetics, Jeju National University)
Lee, Nam Ho (R&D Team, Jeju Industry-University Convergence Center)
Publication Information
Journal of the Korean Applied Science and Technology / v.36, no.4, 2019 , pp. 1259-1267 More about this Journal
Abstract
Anti-inflammatory and anti-oxidative activities were examined on the extract of Dendranthema indicum (D. indicum) flowers. The flowers were extracted two times for 24 h each with 70% ethanol. Upon the biological activities screening, the ethanol extract exhibited potent free radical scavenging activities and inhibited the production of nitric oxide on LPS-induced RAW264.7 macrophages effectively without causing cell toxicity. Further purification by medium pressure liquid hromatography (MPLC) and identification of the isolates led to identification of cynaroside (1) and apigetrin (2). The chemical structures of the isolated compounds were elucidated based on spectroscopic data including nuclear magnetic resonance (NMR) spectra, as well as comparison of the data to the literature values. Also, the quantitative analysis of the compounds was perfromed by high-performance liquid chromatography (HPLC). The isolates 1 and 2 were determined to inhibite the nitric oxide (NO) production dose-dependently. Based on these results, it was suggested that D. indicum extract could be useful as anti-inflammatory agents in cosmetics applications.
Keywords
Dendranthema indicum; Flavonoid; Natural product; Antioxidant; Anti-inflammation;
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