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http://dx.doi.org/10.12925/jkocs.2019.36.2.488

Effects of anti-inflammatory on Perilla frutescens var. crispa Induced by mutants with γ-Ray  

Sim, Boo-Yong (Industry Academic Cooperation Foundation, Joongbu University)
Park, Jung-Hyun (SFC bio Co., LTD)
Kim, Sung-Kyu (SFC bio Co., LTD)
Ji, Joong-Gu (Department of Oriental Health Care, Joongbu University)
Publication Information
Journal of the Korean Applied Science and Technology / v.36, no.2, 2019 , pp. 488-497 More about this Journal
Abstract
The purpose of this study was to confirmed anti-inflammatory effect the apple Induced by mutants with ${\gamma}-Ray$ extract. Cell viability was assessed by MTT assay using RAW 264.7 cells. The extracts measured through changes in the levels of reactive oxygen species (ROS), nitric oxide (NO), inflammatory cytokines, NF-kB, and COX-2 on LPS-induced RAW 264.7 cells. All test results were analyzed by ELISA reader, Luminex and RT-PCR. In result, the extracts was not toxic below in 25 ug/ml, and extracts was inhibited the productions nitric oxide, ROS, cytokines (IL-1b, IL-6, TNF-a), NF-kB and COX-2 in LPS-induced RAW 264.7 cells. Also, the expression levels were decreased on mRNA of $NF-{\kappa}B$ and COX-2. In other words, Perilla frutescens var. crispa Induced by mutants with ${\gamma}-Ray$ extracts showed significant anti-inflammatory effect. These results may be developed as a raw material for new health food and therapeutics to ease the related to the above mediators.
Keywords
anti-inflammatory; safety; Mutation breeding; Perilla frutescens var. crispa ${\gamma}-Ray$;
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