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http://dx.doi.org/10.12925/jkocs.2019.36.1.84

NMR Spectroscopy and Mass Spectrometry of Benzyl Alcohol Galactoside synthesized using β-Galactosidase  

Lee, Hyang-Yeol (Department of Biotechnology, Korea National University of Transportation)
Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)
Publication Information
Journal of the Korean Applied Science and Technology / v.36, no.1, 2019 , pp. 84-89 More about this Journal
Abstract
To characterize the molecular structure of BzO-gal synthesized using Escherichia coli ${\beta}$-gal, NMR ($^1H$- and $^{13}C$-) spectroscopy and mass spectrometry of BzO-gal were conducted. $^1H$ NMR spectrum of BzO-gal showed multiple peaks corresponding to the galactosyl group, which is an evidence of galactosylation on BzOH. Five proton peaks around the aromatic region at ${\delta}_H$ 7.43 ~ 7.24 ppm and 2 peaks from ${\delta}_H$ 4.93 and 4.67 ppm were evidence of the presence of the benzyl group. Seven proton peaks at ${\delta}_H$ 4.32 ~ 3.46 ppm showed the presence of a monosaccharide and were indicative of galactosylation on BzOH. $^{13}C$ NMR spectrum also revealed the presence of 11 carbons suggestive of BzO-gal. The mass value (sodium adduct ion of BzO-gal, m/z = 293.0994) from mass spectrometry analysis of BzO-gal, and $^1H$ and $^{13}C$ NMR spectral data were in good agreement with the expecting structure of BzO-gal. We are expecting that through future study it will eventually be able to develop a new additive of low cytotoxicity.
Keywords
Benzyl alcohol galactoside; ${\beta}$-Galactosidase; Transgalactosylation; NMR spectroscopy; Mass spectrometry;
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