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http://dx.doi.org/10.12925/jkocs.2018.35.1.235

Quantitative Analysis of Paeoniflorin and Paeonol in Peony Extracts and Quality Control Standards  

Yun, Ki-Hun (Department of Industrial Engineering Chemistry, Chungbuk National University)
Chi, Yong-Ha (Research Institute, Soleon, Co. Ltd)
Lee, Dong-Kyu (Department of Industrial Engineering Chemistry, Chungbuk National University)
Paik, Soo-Heui (Department of Pharmacy, Sunchon National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.35, no.1, 2018 , pp. 235-246 More about this Journal
Abstract
Paeony has pharmacological activities such as anti-inflammatory, anti-allergic, anti-bacterial, central inhibitory, gastric secretion inhibition, and antispasmodic activities. In addition, its antioxidant activity and whitening effect being reported, thus it is being explored as raw materials for cosmetics. We compared the changes in the contents of paeoniflorin and paeonol in Peony extracts, depending on the changes of extracting solvents, temperature and time. The HPLC method was set up for simultaneous analysis, the system suitabilities were confirmed by using the calibration curves and the QC samples for each assay batch. Paeonol was detected only in roots, and paeoniflorin was higher in leaf and flower than root. Higher concentrations of both ingredients were extracted when the root was used after grinding to a suitable size, and when 30% 1,3-butylene glycol was used as the extraction solvent. Also the concentrations tended to increase at higher temperature and longer time, but the increase was gradual at over $75^{\circ}C$ and 4 hours. The ratio of root, leaf and flower was determined to be 2+2+1g/0.5kg of batch, reaching the contents criteria of paeoniflorin and paeonol. Finally, we selected as the best extraction condition when the raw materials are mixed with 2+2+1g/0.5kg and extracted with 30% 1,3-butylene glycol as an extraction solvent at $75^{\circ}C$ for 4 hours, considering both the concentrations of two components and the cost of raw materials and manufacturing process, The extraction units were scaled up to 10 kg under this condition.
Keywords
cosmetics; Peony; Paeonia suffruticosa Andrews; solvent extraction; paeoniflorin; paeonol; HPLC;
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