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http://dx.doi.org/10.12925/jkocs.2017.34.2.418

Study of antioxidation activity and melanocyte effect of Pueraria Lobata Root Extract  

Moon, Ji-sun (Department of Beauty Health, Jungwon University)
Lee, Jin-Hee (Department of Culture Service, Dongguk University)
Kim, Young-Bae (Department of Natural Healing, Dongbang Culture University)
Publication Information
Journal of the Korean Applied Science and Technology / v.34, no.2, 2017 , pp. 418-425 More about this Journal
Abstract
This study investigated antioxidation activity through the content of total polyphenol, that of flavonoid and DPPH radical scavenging activity and measured the cytotoxicity against B16F10 melanoma and inhibiting function of melanin biosynthesis to evaluate antioxidation activity and melanocyte effect of pueraria lobata root extract. As the results of study, it was recognized that the toxicity did not show against B16F10 melanoma and the increase of generating melanin was inhibited as the results of measuring the inhibition function of melanin biosynthesis after inducing the generation of melanin by ${\alpha}$-MSH against B16F10 melanoma cell. The high contents of polyphenol and flavonoid was found as the contents of pueraria lobata root extract increases and DPPH radical scavenging activity as the results of antioxidation activity. Through this study, it was recognized that pueraria lobata root extract has the feasibility that can be used as the material of cosmetics as it has the excellent effect of antioxidation activity and inhibiting the generation of melanin against melanocyte, low toxicity against skin cell and its safety against melanocyte of skin was found.
Keywords
Pueraria lobata; Antioxidant; Cosmetics; Melanocyte; Flavonoid;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
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1 Terao, J. "Autoxidation activity of $\beta$-carotene-related carotenoids in solution", Lipids. vol. 24, pp. 657-661, (1989).
2 Chen YT, Zheng RL, Jia ZJ, Ju J. "Flavonoids as superoxide scavengers and antioxidants", Free Radical Biol. Med. vol. 9, pp. 19-21, (1990).
3 Cha, JY, Park SH, Heo JS, Cho YS. "Suppressive effect of administrated glutathione-enriched Saccharomyces cerevisiae FF-8 on the oxidative stress in alcoholic fatty liver", J. Life Sci. vol. 18, pp. 1053-1058, (2008).   DOI
4 Eun YJ, Song YK, Lim HH, Kwon K, Rhim TJ. "Effects of Puerariae Radix extract on the activity of antioxidant. Korea Institute of Herbal Medicine", vol. 12, pp. 53-62, (2007).
5 Shin HJ, Yoo JE, Jung EH, Yoo DY. "Effects of Pueraria lobata on Body Weight and Gene Expression in Obese Rats Muscle with Estrogen Deficiency", THE JOURNAL OF ORIENTAL OBSTETRICS & GYNECOLOGY. vol. 25, pp. 071-084, (2012).
6 Hong JH, Park PS, Lee MY. "Effects of Pucrariae radix extract on Experimentally Alloxan-induced Hyperglycemua in Rabbits", Natural science research. vol. 15, pp. 123-129, (1992).
7 Kim HP. "Anti-inflammatory activity of isoflavonoids from pueraria thunbergiana and biochanin A conjugates", KOSEF., (1994).
8 Han SH. "Effects of Extracts of Pueraria radix on Enzymes Activities of Serum and Lead Level of the Tissues of the Pb-administered Rats", Preventive Nutrition and Food Science, vol. 4, pp. 914-919, (1996).
9 丹野與三太. 葛根の解熱作用に就きて. 日藥物誌. vol. 33, pp. 263-8, (1941).
10 Kim YS, Bu IK. "Effect of Puerariae Radix on Hippocampal Neurons in Ischemic Damaged Rats", Kor. J. Herbology., vol. 19, pp. 77-82, (2004).
11 Ryu BH, Lee BH, Ha MS, Kim DS, Sin DB, Nam KD. "Pueraria lobata Ohwi as an Osteoporosis Therapeutics", Kor. J. FOOD. SCI. TECHNOL. vol. 34, pp. 710-718, (2002).
12 Chang SS, Ostric-Matijasevic B, Oliver AL, Huang CL. "Natural antioxidants from rosemary and sage", J. Food Sci. vol. 42, pp. 1102-1106, (1977).   DOI
13 Farag RS, Badei AZMA, Hewedi FM, EI-Baroty GSA. "Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media", JAOCS. vol. 66, pp. 792-799, (1989).   DOI
14 Frei B. Natural antioxidants in human health and disease. Academic Press. San Diego. vol. 24, pp. 40-55, (1994).
15 Cooke PS, Naaz A. "Role of Estrogensin Adipocyte Development and Function. Experimental biology and medicine", vol. 229, pp. 1127-35, (2004).   DOI
16 Park CH. "Antioxidative components of the roots of Pueraria thunbergiana and its structure-activity relationships", Graduate School of Kyungsung University, (2001).
17 Blois MS. "Antioxidant determinations by the use of a stable free radical", Nature, vol. 181, pp. 1199-1200, (1958).   DOI
18 Folin O, Denis W. "On phosphotungsticphosphomolybdic compounds as color reagents", The Journal of Biological Chemistry, vol. 12, pp. 239-243, (1912).
19 Moreno MI, Isla MI, Sampietro AR, Vattuone MA. "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", Journal of Ethnopharmacology, vol. 71, pp. 109-114, (2000).   DOI
20 Lim HW, Cho NY, Yoon MY, Cha SB, Kim KW, Park YK, Lee JY. "Effect sofcitruse sentialoils melanin productionin B16 melanima cell", J. YakhakHoeji., vol. 47, pp. 25, (2003).
21 Borenfreund E, Puerner JA, "Toxicity determined in vitro by morphologica lalterationsandneutra lred absorption", Toxicol.Lett., vol. 24, pp. 119, (1985).   DOI
22 Cho EK, Gal SW, Choi YJ. "Antioxidative activity and angiotensin converting enzyme inhibitory activity of fermented medical plants(DeulBit) and its modulatory effects of nitric oxide production", J. Appl. Biol. Chem. vol. 53, pp. 91-98, (2010).   DOI
23 Sebastien C, Calliste CA, Mazeron MC, Hantz S, Duroux JL, Rawlison WD, Poly MC, Alain S. "Eight flavonoids and their potential as inhibitors of human cytomegalovirus replication", Antivi. Reserch. vol. 96, pp. 181-186, (2012).   DOI
24 Shao ZH, Hoek TLV, Qin Y, Becher LB. Schumacker T, Li CQ, Dey L, Barth E, Halpern H, Rosen GM, Yuan CS. "Baicalein attenuates oxidant stress in cardiomyocytes", Am J. Physiol. Heart Circ. Physiol. vol. 282, pp. 999-1006, (2002).   DOI
25 Walsh KR, Failla ML. "Transport and metabolism of equol by Caco-2 human intestinal cells", J. Agr. Food Chem. vol. 57, pp. 8297-8302, (2009).   DOI
26 V. Bondet, W. Brand-Williams and C.Berset, "Kinetics and mechanisms of antiocidant activity using the DPPH free radical method", LWT-Food Sci. Technol., vol. 30, pp. 609, (1997)
27 Lee SG, Yu MH, Lee SP, Lee IS. "Antioxidant activities and onduction of apoptosis by methanol extracts from avocado". J. Korean Soc. Food Sci. Nutr. vol. 37, pp. 269-275, (2008).   DOI
28 Chow LW, Wang SJ, Duh PD. Antibacterial activity of burdock. Food Science vol. 24, pp. 195-202, (1997).
29 Duh PD. "Antioxidant activity of burdock (Arctium lappa Linne): Its scavenging effect on free-radical and active oxygen", J. Am. Oil. Chem. Soc., vol. 75, pp. 455-461, (1998).   DOI
30 Kim IC. "Antioxidative Property and Whitening Effect of the Polygoni Multiflori Radix, Polygonati Rhizoma and Ephedrae Herba", J. of Korean Oill Chemists Soc,. vol. 25, pp. 533-538, (2008).
31 Cho WG. "Comparision of Drug Delivery Using Hairless and Pig Skin", J. of Korean Oill Chemiste Soc., vol. 24, pp. 410-417, (2007).
32 Jung SJ, Lee JH, Song HN, Lee SE, Beak NI. "Screening for antioxidant activity of plant medicinal extracts", J. Korean Soc. Appl. Biol. Chem. vol. 47, pp. 135-140, (2005).
33 Kim KB, Yoo KH, Park HY, Jeong JM. "Antioxidative activities of commercial edible plant extracts distributed in korea", J. Korean Soc. Appl. Biol. Chem. vol. 49, pp. 328-333, (2006).
34 Cha JY, Cho YS. "Effect of potato polyphenolics on lipid peroxidation in rats", J. Korean Soc. Food Sci. Nutr. vol. 28, pp. 1131-1136, (1999).
35 Helmersson J, Arnlov J, Larsson A, Basu S. "Low dietary intake of beta-carotene, alpha-tocopherol and ascorbic acid is associated with increased inflammatory and oxidative stress status in a Swedish cohort", Br. J. Nutr. vol. 15, pp. 1-8,. (2008).
36 Koskas JP, Cillard J, Cillard P. Autoxidation of linoleic acid and behavior of its hydroperoxides with and without tocopherols. JAOCS. vol. 61, pp. 1467-1472, (1984).