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http://dx.doi.org/10.12925/jkocs.2015.32.1.108

Antimicrobial and Immunological activities of Vinca minor Extracts  

Kim, Jun-Sub (Department of Biotechnology, Korea National University of Transportation)
Kang, Jo-Eun (Department of Biotechnology, Korea National University of Transportation)
Yu, Il-Hwan (Department of Biotechnology, Korea National University of Transportation)
Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)
Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation)
Lee, Hyang-Yeol (Department of Biotechnology, Korea National University of Transportation)
Publication Information
Journal of the Korean Applied Science and Technology / v.32, no.1, 2015 , pp. 108-115 More about this Journal
Abstract
Vinca alkaloid compounds including vincamine from Vinca minor L. have been extracted by ethanol and hot water extraction methods. Antimicrobial properties of those extracts have been investigated against dandruff causing microorganism Malassezia furfur, yeast, Gram positive and negative bacteria. Vincamine standard and ethanol extract showed no sign of antimicrobial activity, whereas hot-water extract had the activity against M. furfur. Furthermore hot water extract had antimicrobial activity against Gram positive Bacillus sp. and Gram negative Escherichia coli. Cytotoxic properties of those extracts have also been investigated with HaCaT cell (human keratinocyte), HT-29 cell (human colorectal adenocarcinoma cell) and Raw cell, showing no significant cytotoxic effects. We also measured the ROS using dichlorofluorescein diacetate (DCFDA), a popular fluorescence-based probe for reactive oxygen species detection. The result showed the increasement of reactive oxygen species formation (20%) in HaCaT and HT-29 cell lines.
Keywords
Vinca minor extract; Vincamine; Antimicrobial; Immunological effect;
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Times Cited By KSCI : 5  (Citation Analysis)
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