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http://dx.doi.org/10.12925/jkocs.2014.31.1.101

Extracts of Housefly Maggot Reduces Blood Cholesterol in Hypercholesterolemic Rats  

Park, Byung-Sung (Department of Animal Biotechnology, Kangwon National University)
Park, Sang-Oh (Institute of Animal Resources, Kangwon National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.31, no.1, 2014 , pp. 101-112 More about this Journal
Abstract
The aim of this study was to evaluate the biological mechanism of orally administered ethanolic extract of fly maggot(EM) on hypocholesterolemic rats fed a high-cholesterol diet. Sprague Dawley male rats were divided into four groups (EM dose control=0, 5.0, 7.0, and 9.0 mg/100 g BW) and were treated for 6 weeks. EM groups revealed a significant reduction in serum triglyceride, total cholesterol, and LDL-C when compared with the control group(p<0.05). HMG-CoA reductase activity in EM groups were lower than those of the control group, but total sterol, neutral sterol, and bile acid excretion were increased in EM groups when compared with the control group(p<0.05). To identify the biological mechanism of EM towards the hypocholesterolemic effect, sterol response element binding proteins (SREBPs) and the peroxisome proliferator-activated receptors ($PPAR{\alpha}$ transcription system were determined in rats fed a high-cholesterol diet. It was discovered that EM suppress the expression of SREBP-$1{\alpha}$ and SREBP-2 mRNA in the liver tissues of high-cholesterol diet fed rats, while simultaneously increasing the expression of $PPAR{\alpha}$ mRNA(p<0.05). This finding indicates that EM may have hypocholesterolemic effects in rats fed a high-cholesterol diet, by regulating cholesterol metabolism-related biochemical parameters and SREBP-$1{\alpha}$ SREPB-2 and $PPAR{\alpha}$gene expression.
Keywords
Blood lipid; HMG-CoA reductase; sterol; SREPBs; $PPAR{\alpha}$;
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