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http://dx.doi.org/10.12925/jkocs.2013.30.2.215

The preparation of skin analogue composition having the liquid crystalline structure and its cosmetic applications  

Lee, Dong-Kyu (Department of Industrial Engineering Chemistry, College of Engineering, Chungbuk National University)
Jeong, Kwan-Young (Skin research institute, Korea Kolmar Corporation)
Publication Information
Journal of the Korean Applied Science and Technology / v.30, no.2, 2013 , pp. 215-224 More about this Journal
Abstract
Recently, many cosmetic researchers have been focused on the development of high functional cosmetics including anti-wrinkle and whitening. In these studies, they couldn't afford to pay a deep attention to stable encapsulations for unstable materials and efficient drug deliveries for them. Particularly, in order to show a degree of instant effects as cosmetics, they can't also ignore moisturizing effect enough to satisfy customers just after applying and its maintenance by improving the function of skin barrier as well as above two effects. Therefore, skin analogue systems have attracted considerable attention in the view of structural and compositional similarity to intercellular membrane in stratum corneum. And, some models for skin analogue composition were developed to improve the function of skin barrier, stably encapsulate unstable materials such as retinol, vitamin B, C, E, etc., and control their skin penetration in order to show good effects as cosmetics. In this study, we suggest the new skin analogue model having the compositional similarity as well as conventional structural ones. Our skin analogue membrane(SAM) is mainly composed of ceramide/ cholesterol/phosphatidylcholin/fatty acids and its structural defects are compensated by including cholesterol amphiphile and controlling the ratio of ceramide/cholesterol. It was possible to confirm the formation of skin analogue membrane having highly-densed multilamella structure and compare them according to the change of each ratio with a polarized microscope, X-ray diffraction. More detaily, we observed their structures with a electron microscope(TEM). Finally, we dispersed them in excess of continuous water phase, observed the formation of maltese-cross liquid crystalline and measured the efficiency of drug deliveries and moisturizing effects.
Keywords
skin analogue membrane; phosphatidylcholin; Ceramide; Cholesterol amphiphile; multilamella structure; maltese-cross liquid crystalline;
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1 J. A. Bouwstra and P. L. Honeywell- Nguyen, Skin structure and mode of action of vesicles, Adv. Drug Delivery Rev., 54 Suppl. 1, 41-55(2002).   DOI
2 P. W. Wertz and D. T. Dowing, "Stratum corneum: biological and biochemical considerations, in: J. Hadgraft, R. H. Guy, "Transdermal Drug delivery", p. 1-17, Marcel Dekker, NewYork(1989).
3 R. O. Potts and R. H. Guy, Predicting skin permeability, Pharm. Res., 9, 663-669(1992).   DOI
4 P. W. Wertz, The nature of the epidermal barrier: Biochemical aspects, Adv. Drug Del. Rev., 18(3), 283-294(1996).   DOI
5 P. W. Wertz and B. A. I. van den Bergh, The physical, chemical and functional properties of lipids in the skin and other biological barriers, Chem. Phys. Lipids., 91, 85-96(1998)   DOI
6 D. C. Swartzendruber, Studies of epidermal lipids using electron microscopy, Semin. Dermatol., 11, 157-161(1992).
7 B. F. van Duzee, Thermal analysis of human stratum corneum, J. Invest. Dermatol., 65, 404-408(1975).   DOI
8 S. H. White, D. Mirejovsky and G. I. King, Structure of lamellar lipid domains and corneocytes envelopes of murine stratum corneum. An X-ray diffraction study, Biochemistry, 27, 3725-3732(1988).   DOI
9 S. Y. Hou, A. K. Mitra, S. H. White, G. K. Menon, R. Ghadially and P. Elias, Membrane structure in normal and essential fatty acid-deficient stratum corneum; characterization of ruthenium tetroxide stanning and X-ray diffraction, J. Invest. Dermatol., 96, 215-223(1991).   DOI
10 G. Cevc and G. Blume, Lipid vesicles penetrate into intact skin owing to transdermal osmotic gradients and hydration force, Biochim. Biophys. Acta., 1104, 226-232(1992).   DOI
11 D. C. Swarzendruber, P. W. Wertz, D. J. Kitko, K. C. Madison and D. T. Dowing, Molecular models of the intercellular lipid lamellae in mammalian stratum corneum, J. Invest. Dermatol., 92, 251-257(1989).   DOI
12 D. Kuempel, D. C. Swarzendruber, C. A. Squier and P. W. Wertz, In vitro reconstitution of stratum corneum lipid lamellae, Biochim. Biophys. Acta., 1372, 135-140(1998).   DOI
13 B. A. I. van den Bergh, D. C. Swarzendruber and A. Bos-van der Geest et al., Development od an optimal protocol for the ultrastructural examination of skin by transmission electron microscopey, J. Microsc., 187, 125-133(1997)   DOI
14 L. Lehmann, S. Keipert and M. Gloor, Effects of microemulsion on the stratumcorneum and hydrocortisone penetration, Eur. J. Plarm. Biopharm., 52, 129-136(2001)   DOI
15 P. R. Rege, V. D. Vilivalam and C. C. Collins, Development in release testing of topical dosage forms: use of the Enhancer cell with a sampling, J pharm and biochem. anal., 17, 1225-1233(1998).   DOI
16 T. de Vringer, J. G. H Joosten and H. E. Junginger, A study of gel structure in a nonionic O/W cream by X-ray diffraction and microscopic methods, Colloid & Polymer sci, 265,167-179(1987).   DOI
17 A. Sylvia, Wissing and Rainer H. Muller, The influence of solid lipid nanoparticles on skin hydration and viscoelasticity - in vivo study, Eur. J. Plarm. Biopharm., 56, 67-72(2003).   DOI
18 J. Pinnagoda, R. A. Tupker, T. Agner and J. Serup, Guidelines for transepidermal water loss(TEWL) measurement, Contact Dermatitis., 22, 164-178(1990).   DOI
19 T. de Vringer, J. G. H Joosten and H. E. Junginger, A study of gel structure in a nonionic O/W cream by differential scanning calorimetry, Colloid & Polymer sci., 264, 691-700(1986).   DOI