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http://dx.doi.org/10.12925/jkocs.2005.22.1.13

The Structure Change Study on the Actin-Myosin Cross-Bridges in SH of Myosin Head by The Computer Data  

Kim, Duck-Sool (Department of Computer Engineering Tongmyong University of Information Technology)
Ok, Soo-Yol (Department of Computer Engineering Tongmyong University of Information Technology)
Park, Keun-Ho (Department of Chemical Engineering, Changwon National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.22, no.1, 2005 , pp. 84-90 More about this Journal
Abstract
IASL(iodo acetamide) and MSL(maleimide) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. Equatorial refiection(10,11) change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of 143${\AA}$ and 72${\AA}$ could offer information of the mass projection of population of myosin heads along the :filament axis. The slope of intensity profile of the mass projection of 143${\AA}$ and reflection of IASL is appeared and that of MSL is appeared sharply. The decrease of 215${\AA}$ reflection intensity is appeared the periodical characteristic of 143${\AA}$ reflection by spin label. The raise of MSL actin reflection at 51${\AA}$ and 59${\AA}$ in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we could conclude that LASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.
Keywords
myosin head; spin label; reflection;
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  • Reference
1 H. E. Huxley, A. R. Farqi, and M. Brown, The Low-Angle X-ray Diagram of Vertebrate Striated Muscle and Its Behaviour during Contraction and Rigor, J. Mol. Biol., 30, 383 (1967)
2 H. E. Huxley, R. M. Simmons, A. R. Farqi, and M. Kress, X-ray Diffraction Studies on Muscle during Shortening and Their Implications Concerning Crossbridge Behaviour, Adv. Exp. Med. Biol., 266, 347 (1988).
3 M. A. Bagni, G. Cecchi, F. Coloma, and C. Poggesi, Tension and Stiffness of Frog Muscle fibers at Full Filament Overlap, J. Muscle Res. Cell Motil., 11, 371 (1990)
4 H. Yagi, S. Takemori, and M. Yamaguchi, An X-ray Diffraction Study of Frog Skeletal Muscle during Shortening near The Maximum Velocity, J. Mol. Biol., 231, (1993)
5 K. Hirose, T. J. M. Murray, C. Franzini-Armstrong, and Y. E. Goldman, Structural Changes in Muscle crossbridge accompanying Force Generation, J. Cell Biol., 127, 763 (1994)
6 K. Wakabayashi, H. Saito, T. Kobayashi, Y. Ueno, and H. Tanaka, Detection of The Spacing Changes of Muscle Thin Filaments during Force Generation by X-ray diffraction, Photon Factory Act. Rep., 10, 352 (1992)
7 I. Matsubara and N. Yagi, Structural Change m The Thin Filament during Activation Studied by X-ray Diffraction by Highly Stretched Skeletal Muscle, J. Mol. Biol., 208, 359 (1989)
8 H. E. Huxley, A. R. Farqi, M. Kress, J. Bordas, and M. H. J. Koch, Time Resolved X-ray Diffraction Studies of The Myosin Layer-Line Reflections during Muscle Contraction, J. Mol. Biol., 158, 637 (1982)
9 H. E. Huxley, Mechanical Transients and the Origin of Muscular Force, Science, 164, 1365 (1969)   DOI   PUBMED   ScienceOn
10 K. Wang, R. McCarter, J. Wright, J. Beverly, and R. Ramirez - Mitchel, Viscoelasticity of the Sarcomere Matrix of Skeletal Muscle Contraction The Actin-Myosin Composite Filament is a Dual-Stage Molecular Spring, Biophys. J., 64, 1161 (1993)
11 M. Kress, H. E. Huxley, A. R. Faruqi, and J. Hendrix, Structural Changes during Activation of Frog Muscle Studies by Time-Resolved X-ray Diffraction, J. Mol. Biol., 188, 325 (1986)
12 N. Yagi, Effects of N-ethlmaleimide on The Structure of Skinned Frog Skeletal Muscle, J. Muscle Res. Cell Matil., 13, 457 (1992)
13 Y. Amemiya, K. Wakabayashi, T. Hamanaka, T. Wakabayashi, T. Matsushta, and H. Hashizume, Design of a Small-Angle X-ray Diffractometer usmg Synchrotron Radiation at The Photon Factory, Nucl. Instrum. Methods, 208, 471 (1983)
14 K. Wakabayashi. H. Tanaka, T. Kobayashi. T. Hamanaka, S. Nishizawa, H. Sugi, and T. Mitsui. Dynamic X-ray Diffraction of Skeletal Muscle Contraction Structural Change of Actin Filaments, Adv. Biophys., 27, 3 (1991)
15 H. Iwamoto, T. Kobayashi, Y. Amemiya, and K. Wakabayashi, Effect of Stretch and Release on Equatorial X-ray Diffraction During a Twitch Contraction of Frog Skeletal Muscle, Biophys. J., 68, 227 (1995)
16 N. Yagi and I. Matsubara, 'Structural Changes in The Thin Filament during Activation Studied by X-ray Diffraction by Highly Stretched Skeletal Muscle, J. Mol. Biol., 208, 359 (1989)
17 K. Wakabayashi and Y. Amemiya, 'Handbook on Synchrotron Radiation.', pp. 597, vol. 4. Progress in X-ray Synchrotron Diffraction Studies of Muscle Contraction, North Holland. Amsterdam (1991)