Browse > Article
http://dx.doi.org/10.12925/jkocs.2004.21.2.6

Response Surface Analysis of Dietary n-3/n-6 and P/S Ratio on Reduction of Plasma Lipids in Rats  

Park, Byung-Sung (Animal Life Science Program, Collage of Animal Resources and Science, Kangwon National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.21, no.2, 2004 , pp. 148-155 More about this Journal
Abstract
Response surface analysis was used to study dietary ratios of n-3/n-6 fatty acid and P/S to minimize plasma triglycerides, total cholesterol and LDL ${\cdot}$ VLDL-C levels and maximize plasma HDL ${\cdot}$ C levels of rats. Because the dietary components were not statistically independent, they were studied in combinations of two variables. The two-variable combinations were the most useful in locating the desired maximum or minimum plasma triglycerides, total cholesterol and LDL ${\cdot}$ VLDL-C response in terms of the proportions of the dietary components. Response surface contours and three dimensional plots were developed for each plasma lipid response. The contours and three dimensional plots were used to help determine those combinations of the dietary fatty acid ratios that would produce the desired minimum or maximum lpid responses. The statistical analyses indicated that the minimized plasma cholesterol response levels could be attained with a diet consisting of 2.26 n-3/n-6 fatty acid and 2.15 P/S ratios.
Keywords
dietary n-3/n-6 ratio; dietary P/S ratio; plasma cholesterol; lipoprotein; response surface analysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 M. B. Moir, V. A. Zammit, and B. S. Park, Quantification in Vivo of the Effects of Different Types of Dietary Fat on the Loci of Control Involved in Hepatic Triacyglycerol Secretion, Biochem. J., 308, 537 (1995)   DOI
2 W. E. Connor, Importance of n-3 Fatty Acids in Health and Disease, Am. J, Clin, Nutr., 71, 171S (2000)
3 J. R. Stewart, E. B. Fryer, and H. C. Fryer, Effects of Dietary Fiber, Carbohydrate, Lipid and Protein Levels on Serum and Liver Lipids in Rats, J. Nutr., 117, 650 (1987)   DOI
4 M. Toyomizu, Y. Akiba, T. Matsumoto, and M. Hohguchi, Response Surfaces of Body Protein and Energy Gains in Growing Chicks Fed Diets over the Entire Range of Compositions of Protein, Fat and Carbohydrate, J. Nutr., 115, 61 (1985)
5 J. M. Folch, M. Lees, and G. A. S. Stanley, A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. BioI. Chem., 226, 497 (1957)
6 R. H. Myers, Response . Surface Methodology, P. 127, Allyn and Bacon Inc., Boston (1971)
7 SAS, SAS User's Guide : Statistics, Version 8 ed, SAS Institute INC., Cary, NC, USA (2001)
8 N. Yamada, T. Takita, and M, Wada, Effects of Dietary n-3/n-6 and Polyunsaturated Fatty Acid/Saturated Fatty Acid Ratios on Platelet Aggregation and Lipid Metabolism in Rats, J. Nutr. Sci. VitaminoI., 42, 423 (1996)   DOI   ScienceOn
9 B. S. Park, Combination Effects of Various Dietary Ratios n-3/n-6 and PUFA/SFA on Cholesterol Metabolism in Rats, Kor. J. Food Sci. Ani. Resour., 16, 79 (1996)
10 T. Takita, K. Nakamura, K, Suzuki, and S. Innami, Effect of n-3/n-6 Polyunsaturated Fatty Acid Ratios on Lipids Metabolism in Rats Fed on Hypercholesterolemic Diets, J. JPn. Soc Nutr. Food Sci., 45, 317 (1992)   DOI
11 L. A. Carlson and L. E. Bettinger, Ischemic Heart Disease in Relation to Fasting Values of Plasma Triglyceride and Cholesterol, Lancet., 1, 865 (1972)
12 American Institute of Nutrition, Report of the American Institute of Nutrition Ad Hoc Committee on Standards for Nutritional Studies, J. Nutr., 107, 1340 (1977)
13 M. Glovanni, Response Surface Methodology and Product Optimization, Food Tech, 37, 41 (1983)
14 E. Mantzioris, L. G. Cleland, R. A. Gibson, and M. J. James, Biochemical Effects of a Diet Containing Foods Enriched with n-3 Fatty acids, Am. J. CIin. Nutr., 72, 42 (2000)
15 B. S. Park and Y. C. Rhee, Correlation between Ratios of Dietary $\omega3/\omega6$, PUFA/SFA, MUFA/SFA and Contents of Plasma, Muscle, Liver Fatty Acid and Cholesterol in Rat, Kor. J. Anim. Sci., 36, 312 (1994)
16 W. R. Morrison, and L. M. Smith, Preparation of Fatty Acid Methylesters and Dimethylacetals from Lipid with Boron Fluoride-methanol, J. Lipid Res., 5, 600 (1964)
17 Statistical Graphics Corporation : User's Guide, Statgraphics, Statistical Graphics System, STSC Inc., USA (1987)
18 T. Takita, K. Nakamura, and S. Innami, Effects of Dietary Fats with Diferent n-3 Polyunsaturated Fatty Acid and n-6 Polyunsaturated Fatty Acid on Lipid Metabolism in Rats, JPn. J. Nutr., 47, 141 (1989)   DOI
19 B. S. Park, B. D. Ko, Y. C. Rhee, and N. H. Lee, Response Surface Analysis of the Dietary n-3/n-6, PUFA/SFA and MUFA/SFA Ratios on Cholesterol Levels of Muscle and Liver in Rats, Kor. J. Anim Nutr. Feed., 19, 345 (1995)
20 M. Y, Abeywardena, P, L. Molenman, and J. S. Charnock, Long-term Saturated Fat Supplementation in the Rat Cause an Increase in $PGI_{3}$ /$TXB_{2}$ Ratio of Platelet and Vessel Wall Compared to n-3 and n-6 Dietary Fatty Acids, Atherosclerosis., 66, 181(1987)   DOI   ScienceOn
21 H. J. Lee, H. K. Kim, and H. M. Choi, Different Sources of ${\omega}3$ Fatty Acids at the Fixed Ratio of P/S Affect Glutathione Dependent Enzymes in Rat Hepatocarcinogenesis, Kor. J. Nutr., 36, 785 (2003)
22 S. H. Kim, W. K. Kim, and C. E. Chung, The Effects of n-6/n-3 and P/S Ratio of Dietary Lipid on Lipid Metabolism of Rats at Different Age, Kor. J. Nutr., 27, 687 (1994)