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The Analysis of Free Amino Acids in Tobacco  

Lee, Jeong-Min (KT&G Research Institute)
Min, Hey-Jung (KT&G Research Institute)
Jang, Gi-Chul (KT&G Research Institute)
Hwang, Keon-Joong (KT&G Research Institute)
Publication Information
Journal of the Korean Society of Tobacco Science / v.32, no.2, 2010 , pp. 70-76 More about this Journal
Abstract
A simple and sensitive automatic amino acid analyzer method for the determination of free amino acids in tobacco was described. Sample preparation consisted of a single step of extraction with 0.1 mol HCl at ambient temperature in 60 min by sonication, followed by filtration of an aliquot. Automated amino acid analyzer was used to construct a post-column ninhydrin reaction unit to monitor amino acids separated by liquid chromatography using a series of eluting buffers. By optimization of sample preparation, separation of 19 amino acids was achieved. Limits of quantitation was 0.01 mg/g, coefficients of variation ranged from 0.5 % to 8.9 % and recoveries range from 85 % to 106 %. The method was applied to the analysis of amino acids contents of tobacco leaves in different varieties.
Keywords
free amino acids; ninhydrin; automatic amino acid analyzer;
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