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Characteristics of Potato Virus Y (PVY) Mutant Isolated from PVY Resistance Breeding Line in Korea  

Kim, Jae-Hyun (Bio-resources Research Group, KT&G Central Research Institute)
Kuem, Wan-Soo (Bio-resources Research Group, KT&G Central Research Institute)
Lee, Sin-Ho (Dept. of Horticultural Research Institute, Rural Development Administration)
Kim, Jeong-Soo (Dept. of Horticultural Research Institute, Rural Development Administration)
Jeon, Yong-Ho (Bio-resources Research Group, KT&G Central Research Institute)
Jung, Suk-Hun (Bio-resources Research Group, KT&G Central Research Institute)
Chung, Youl-Young (Bio-resources Research Group, KT&G Central Research Institute)
Park, Yong-Hack (Bio-resources Research Group, KT&G Central Research Institute)
Publication Information
Journal of the Korean Society of Tobacco Science / v.28, no.2, 2006 , pp. 100-110 More about this Journal
Abstract
A mutant of Potato vims Y (PVY) was occurred in PVY resistance flue-cured tobacco breeding line KF0402 $(TC1146{\times}KF117)$ showing vein necrosis at Suwon in Korea. This isolate, PVY-SWM, was differentiated from other PVY based on biological properties and nucleotide sequence analyses of coat protein gene. PVY-SWM caused typical symptoms on 21 indicator plants as compared to the PVY-TOJC37. Remarkably, the PVY-SWM induced distinctly different symptom of systemic vein necrosis on tobacco cultivars V.SCR, PBD6, TN86, TN90, Virgin A Mutant (VAM), Wislica, NC744, KB108 and KB111, which were reported to have the recessive potyvirus resistance gene va. In RT-PCR assays with specific primers for detection of PVY, a single band of about 800bp in length was produced. The amplified DNA was cloned and the nucleotide sequence was determined. The coat protein gene of PVY-SWM showed 88.4%-99.0% and 92.5%-98.5% identities to the 12 different PVY isolates of Genbank Database at the nucleotide and amino acidi respectively. Multiple alignments as well as cluster dendrograms of PVY-SWM isolate revealed close phylogenetic relationship to the $PVY^{NTN}$ subgroup.
Keywords
Potato virus Y; tobacco; potyvirus resistance; mutant;
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1 Canto, T., Lopez-Moya, J., Serra-Yoldi, M., Diaz-Ruiz, J. and Lopez-Abella, D. (1995) Different helper component mutations associated with lack of aphid transmissibility in two isolates of Potato virus Y. Phytopathology 85:1519-1524   DOI
2 de Bokx, J.A. and Huttinga, H. (1981) Potato virus Y. CMI/AAB Description of Plant Viruses, No 242
3 Koelle, G. (1958) Versuche zur Vererbung der Krankheitsresistenz bei Tabak; 2 mitt eine rippen-brune-resistente Virgin A Mutante nach Anwendung knstlicher Mutations aulsung durch Rtgenstrahlen. Tabak-Forschung 24:83-84
4 Miller, R.D. (1987) Registration of TN 86 burley tobacco. Crop. Sci. 27:365-366
5 Singh, M. and Singh, R.P. (1996) Nucleotide sequence and genome organization of a Canadian isolate of the common strain of Potato virus Y (PVY-O). Can. J. Plant Pathol. 18:209-214   DOI   ScienceOn
6 van den Heuvel, J.F. J.M., van der Vlugt, R.A.A., Verbeek, M., DeHaan, P.T. and Huttinga, H. (1994) Characteristics of a resistance-breaking isolate of Potato virus Y causing potato tuber necrotic ringspot disease. Eur. J. Plant Pathal. 100:347-356   DOI
7 금완수, 정윤화, 정석훈, 최상주, 이승철 (1991) N. africana의 감자바이러스 Y 엽맥괴저 계통에 대한 저항성 유전. 한국연초학회지 13:48-51
8 Cheong, S.R., Choi, J.K., Yoshida, K. and Lee, B.Y. (1995) Complementary DNA cloning and sequencing of the coat protein gene of Potato virus Y-Ordinary Korean strain. Korean J. Plant Pathol. 11:73-79
9 Lewis, R.S. (2005) Transfer of resistance to Potato virus Y (PVY) from Nicotiana africana to Nicotiana tabacum: possible influence of tissue culture on the rate of introgression. Theor. Appl. Genet. 110:678-687   DOI
10 Robaglia, C., Durand-Tardif, M., Tronchet, M., Boudazin, G., Astier-Manifacier, S. and Casse-Delbart, F. (1989) Nucleotide sequence of Potato virus Y (N Strain) genomic RNA. J. Gen. Virol. 70:935-947   DOI   ScienceOn
11 Masuta, C., Nishimura, M., Morishita, H. and Hataya, T. (1999) A single amino acid change in viral genome-associated protein of potatovirus Y correlates with resistance breaking in 'Virgin A Mutant' tobacco. Virology 89:118123
12 Joung, Y.H., Jeon, J.H., Choi, K.H., Kim, H.S., Yi, Y.S. and Joung, H. (1997) An effective detection of Potato virus Y using RT-PCR technique. Korean J. Plant Pathol. 13:219-224
13 정석훈, 조천준, 최상주, 조명조 (1998) 연초 버어리종 웅성불임 일대잡종 KB111의 육성경과 및 농경적 특정. 한국연초학회지 20:153-159   과학기술학회마을
14 Miller, R.D. (1991) Registration of TN 90 burley tobacco. Crop. Sci. 31: 852   DOI
15 Le Romancer, M., Nedelee, M. (1994) Biological characterization of various geographical isolates of Potato virus Y inducing superficial necrosis on potato tubers. Plant Pathol. 43:138-144   DOI   ScienceOn
16 Xiong, Z. and Acosta-Leal, R. (2005) Resistance to and Resistance-breaking of Potato Virus Y. http://ag.arizona.edu/~zxiong/pvy.html. Dept. of Plant Pathology, University of Arizona
17 정석훈, 최상주, 조천준, 김대송, 조명조, 이승철 (1994) 버어리종 연초 신품종 KB 108의 육성 및 특성. 한국연초학회지 16:57-63
18 Thole, V., Dalmay, T., Burgyan, J. and Balazs, E. (1993) Cloning and sequencing of Potato virus Y (Hungarian isolate) genomic RNA. Gene 123:149-156   DOI   ScienceOn
19 Park, E.K., Kim, J.J. and Boo, K.S. (1984) Two new PVY strains isolated from tobacco plant in Korea. Korean J. Plant Pathol. 23:209-214   과학기술학회마을
20 Weidemann, H.L. (1988) Importance and control of Potato virus Y-N (PVY-N) is seed potato production. Potato Res. 331:85-94
21 Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Eds. New York, Cold Spring Harbor
22 Riechmann, J.L., Lain, S. and Garcia, J.A. (1992) Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 73:1-16   DOI   ScienceOn
23 van der Vlugt, R.A.A., Leunissen, J. and Goldbach, R. (1993) Taxonomic relationships between distinct Potato virus Y isolates based on detained comparisons of the viral coat proteins and 3' nontranslated regions. Arch. Virol. 131:361-375   DOI
24 Cho, H.S., Jun, S.I., Kwon, S.Y., Park, E.K., Paek, K.Y. and Paek, K.H. (1994) Isolation and nucleotide sequence analysis of a partial cDNA clone for Potato virus Y-VN (Korean isolate) genome. Mol. Cells 4: 143-148