Browse > Article
http://dx.doi.org/10.5338/KJEA.2021.40.4.36

Degradation effect of carbendazim in soil by application with the microbial agent, Rhodococcus sp. 3-2  

Yeon, Jehyeong (Bioremedation Team, National Institute of Agricultural Sciences)
Kim, Hyeon-su (Bioremedation Team, National Institute of Agricultural Sciences)
Ahn, Jae-Hyung (Bioremedation Team, National Institute of Agricultural Sciences)
Han, Gui Hwan (Center for Industrialization of Agricultural and Livestock Microorganisms)
Oh, Young Goun (Eco-Friendly Agri-Bio Research Center, Jeonnam Bioindustry Foundation)
Cho, Il Kyu (Eco-Friendly Agri-Bio Research Center, Jeonnam Bioindustry Foundation)
Park, In-Cheol (Bioremedation Team, National Institute of Agricultural Sciences)
Publication Information
Korean Journal of Environmental Agriculture / v.40, no.4, 2021 , pp. 322-329 More about this Journal
Abstract
BACKGROUND: The fungicide of benomyl, a benzimidazole group, has been commonly used for pesticides against fungal diseases in the world. However, benomyl is rapidly hydrolyzed in the environment after using to control plant diseases and has adverse effects by generating carbendazim, which is toxic to plants, humans, and the environment. METHODS AND RESULTS: In this study, the decomposition effect of carbendazim, a degradation product of benomyl was conducted in pot and field after making a prototype of benomyl-degrading microbial agent (BDMA). We found that the carbendazim-degrading microbial agent (CDMA) (105, 106, and 107 cfu/g soil) decomposed carbendazim by 50% or more in all the treatments, compared to the untreated control in the pot tests after four weeks. The effect of 100% decomposition of carbendazim was observed at 7 days after treatment, when the prototype of BDMA was apllied at 10-folds dilution in the field. The decomposition effect at more than 60% and plant growth promoting effect were observed after 7 days of the treatment, compared with the untreated group in the second field experiment,treated with commercially available concentrations of 500-folds and 1,000-folds. CONCLUSION(S): These results might represent that the BDMA would decompose carbendazim effectively, a decomposition product of the fungicide benomyl, remaining in agricultural area, and it could be utilized practically by using a low dilution rate.
Keywords
Benomyl; Carbendazim; Decomposition effect; Rhodococcus sp. 3-2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Pinto CG, Laespada MEF, Martin SH, Ferreira AMC, Pavon JLP, Cordero BM (2010) Simplified QuEChERS approach for the extraction of chlorinated compounds from soil samples. Talanta, 81, 385-391. https://doi.org/10.1016/j.talanta.2009.12.013.   DOI
2 Adedara I, Vaithinathan S, Jubendradass R, Mathur P, Farombi E (2013) Kolaviron prevents carbendazim-induced steroidogenic dysfunction and apoptosis in testes of rats. Envirnmental Toxicology and Pharmacology, 35, 444-453. https://doi.org/10.1016/j.etap.2013.01.010.   DOI
3 Branford MVP, Cruz EDL, Solano K, Ramirez O (2013) Pesticide exposure on sloths (Bradypus variegatus and Choloepus hoffmanni) in an agricultural landscape of Northeastern Costa Rica. Journal of Environmental Biology, 35, 29-34.
4 Sharma P, Sharma M, Raja M, Singh DV, Srivastava M (2016) Use of Trichoderma spp. in biodegradation of Carbendazim. Indian Journal of Agricultural Sciences, 86(7), 891-894.
5 Fang H, Wang Y, Gao C, Yan H, Dong B, Yu Y (2010) Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim. Biodegradation, 21, 939-946. https://doi.org/10.1007/s10532-010-9353-0.   DOI
6 Negi G, Pankaj, Srivastava A, Sharma A (2014) In situ Biodegradation of endosulfan, imidacloprid, and carbendazim using indigenous bacterial cultures of agriculture fields of Uttarakhand, India. World Academy of Science, Engineering and Technology, 8(9), 898-906.
7 Bai N, Wang S, Abuduaini R, Zhang M, Zhu X, Zhao Y (2017) Rhamnolipid-aided biodegradation of carbendazim by Rhodococcus sp. D-1: Characteristics, products, and phytotoxicity. Science of the Total Environment, 590, 343-351. http://dx.doi.org/10.1016/j.scitotenv.2017.03.025.   DOI
8 Silambarasan S, Abraham J (2020) Biodegradation of carbendazim by a potent novel Chryseobacterium sp. JAS14 and plant growth promoting Aeromonas caviae JAS15 with subsequent toxicity analysis. 3 Biotech, 10, 336. https://doi.org/10.1007/s13205-020-02319-w.   DOI
9 Laal F, Hormozi M, Madvari RF, Noorizadeh N, Chahak AF (2017) Health risk assessment of occupational exposure to harmful chemical agents in a pesticide manufacturing plant. Journal of Occupational Health and Epidemiology, 6(3), 171-177. https://doi:10.29252/johe.6.3.171.   DOI
10 Wang L, Wang Y, Han L, Wang M, Han X, Feng J (2017) The efficacy and translocation behavior of carabrone in wheat and cucumber. Crop Protection, 100, 87-95. https://doi.org/10.1016/j.cropro.2017.06.010.   DOI
11 Lee J, Jeon Y, Jung M, Kim Y, Park I, You J, Lee C, Han B, An S, Ahn J (2020) Isolation and characterization of Rhodococcus sp. strains capable of degrading benzimidazole fungicides benomyl and carbendazim. The Korean Society of Pesticide Science, 24(2), 163-171. https://doi.org/10.7585/kjps.2020.24.2.163.   DOI
12 Falciglia PP, De Guidi G, Catalfo A, Vagliasindi FGA (2016) Remediation of soils contaminated with PAHs and nitro-PAHs using microwave irradiation. Chemical Engineering Journal, 296, 162-72. https://doi.org/10.1016/j.cej.2016.03.099.   DOI
13 Gevao B, Semple KT, Jones KC (2000) Bound pesticide residues in soils: A review. Environmental Pollution, 108(1), 3-14. https://doi.org/10.1016/S0269-7491(99)00197-9.   DOI
14 Yoon P, Ko S (2019) Studies on toxicological evaluation of pesticides (fungicide, insecticide, herbicide) using tree frog embryos, hyla japonica. Korean Journal of Environment and Ecology, 33(2), 178-186. https://doi.org/10.13047/KJEE.2019.33.2.178.   DOI
15 Xu X, Chen J, Li B, Tang L (2018) Carbendazim residues in vegetables in China between 2014 and 2016 and a chronic carbendazim exposure risk assessment. Food Control, 91, 20-25. https://doi.org/10.1016/j.foodcont.2018.03.016.   DOI
16 Hwang L, Park S (2019) Monitoring and risk assessment of carbendazim residues in soybean sprout and mungbean sprout from markets in Western Seoul. Journal of Food Hygiene and Safety, 34(4), 348-353. https://doi.org/10.13103/JFHS.2019.34.4.348.   DOI
17 Tao H, Bao Z, Jin C, Miao W, Fu Z, Jin, Y (2020) Toxic effects and mechanisms of three commonly used fungicides on the human colon adenocarcinoma cell line Caco-2. Environmental Pollution, 263, 114660. https://doi.org/10.1016/j.envpol.2020.114660.   DOI
18 Zhu Z, Zhou F, Li J, Zhu F, Ma H (2016) Carbendazim resistance in field isolates of Sclerotinia sclerotiorum in China and its management. Crop Protection, 81, 115-121. https://doi.org/10.1016/j.cropro.2015.12.011.   DOI
19 Odukkathil G, Vasudevan N (2013) Toxicity and bioremediation of pesticides in agricultural soil. Reviews in Environmental Science and Biotechnology, 12, 421-444. https://doi.org/10.1007/s11157-013-9320-4.   DOI
20 Jia JL, Wang BB, Wu Y, Niu Z, Ma XY and Yu Y (2016) Environmental risk controllability and management of VOCs during remediation of contaminated sites. Soil and Sediment Contamination: An International Journal, 25, 13-25. https://doi.org/10.1080/15320383.2016.1085834.   DOI
21 Vidhya Lakshmi C, Mohit K, Sunil K (2009) Biodegradation of Chlorpyrifos in soil by enriched cultures. Curr Microbiol, 58, 35-38. https://doi.org/10.1007/s00284-008-9262-1.   DOI
22 Reddy GV, Antwi FB (2016) Toxicity of natural insecticides on the larvae of wheat head armyworm, Dargida diffusa (Lepidoptera: Noctuidae). Environmental Toxicology and Pharmacology, 42, 156-162. https://doi.org/10.1016/j.etap.2016.01.014.   DOI
23 Morillo E, Villaverde J (2017) Advanced technologies for the remediation of pesticide-contaminated soils. Science of The Total Environment, 586, 576-597. https://doi.org/10.1016/j.scitotenv.2017.02.020   DOI
24 Chuang S, Yang H, Wang X, Xue C, Jiang J, Hong Q (2021) Potential effects of Rhodococcus qingshengii strain djl-6 on the bioremediation of carbendazim-contaminated soil and the assembly of its microbiome. Journal of Hazardous Materials, 414, 125496. https://doi.org/10.1016/j.jhazmat.2021.125496.   DOI