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http://dx.doi.org/10.5338/KJEA.2021.40.3.23

Effect of Non-indigenous Bacterial Introductions on Rhizosphere Microbial Community  

Nogrado, Kathyleen (Department of Bioenvironmental Chemistry, Jeonbuk National University)
Ha, Gwang-Su (Microbial Institute for Fermentation Industry (MIFI))
Yang, Hee-Jong (Microbial Institute for Fermentation Industry (MIFI))
Lee, Ji-Hoon (Department of Bioenvironmental Chemistry, Jeonbuk National University)
Publication Information
Korean Journal of Environmental Agriculture / v.40, no.3, 2021 , pp. 194-202 More about this Journal
Abstract
BACKGROUND: Towards achievement of sustainable agriculture, using microbial inoculants may present promising alternatives without adverse environmental effects; however, there are challenging issues that should be addressed in terms of effectiveness and ecology. Viability and stability of the bacterial inoculants would be one of the major issues in effectiveness of microbial pesticide uses, and the changes within the indigenous microbial communities by the inoculants would be an important factor influencing soil ecology. Here we investigated the stability of the introduced bacterial strains in the soils planted with barley and its effect on the diversity shifts of the rhizosphere soil bacteria. METHODS AND RESULTS: Two different types of bacterial strains of Bacillus thuringiensis and Shewanella oneidensis MR-1 were inoculated to the soils planted with barley. To monitor the stability of the inoculated bacterial strains, genes specific to the strains (XRE and mtrA) were quantified by qPCR. In addition, bacterial community analyses were performed using v3-v4 regions of 16S rRNA gene sequences from the barley rhizosphere soils, which were analyzed using Illumina MiSeq system and Mothur. Alpha- and beta-diversity analyses indicated that the inoculated rhizosphere soils were grouped apart from the uninoculated soil, and plant growth also may have affected the soil bacterial diversity. CONCLUSION: Regardless of the survival of the introduced non-native microbes, non-indigenous bacteria may influence the soil microbial community and diversity.
Keywords
Bacillus thuringiensis; Shewanella oneidensis MR-1; Rhizosphere Bacterial Diversity;
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1 Backer R, Rokem JS, Ilangumaran G, Lamont J, Praslickova D, Ricci E, Subramanian S, Smith DL (2018) Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science, 9, 1473. https://doi.org/10.3389/fpls.2018.01473.   DOI
2 Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD (2013) Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Applied and Environmental Microbiology, 79, 5112-5120. https://doi.org/10.1128/aem.01043-13.   DOI
3 Prasanna R, Chaudhary V, Gupta V, Babu S, Kumar A, Singh R, Shivay YS, Nain L (2013) Cyanobacteria mediated plant growth promotion and bioprotection against Fusarium wilt in tomato. European Journal of Plant Pathology, 136, 337-353. https://doi.org/10.1007/s10658-013-0167-x.   DOI
4 Yue JC, Clayton MK (2005) A similarity measure based on species proportions. Communications in Statistics - Theory and Methods, 34, 2123-2131. https://doi.org/10.1080/STA-200066418.   DOI
5 Lozupone C, Lladser ME, Knights D, Stombaugh J, Knight R (2011) UniFrac: an effective distance metric for microbial community comparison. The ISME Journal, 5, 169-172. https://doi.org/10.1038/ismej.2010.133.   DOI
6 Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL (2012) Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics, 13, 134. https://doi.org/10.1186/1471-2105-13-134.   DOI
7 Nogrado K, Lee S, Chon K, Lee J-H (2019) Effect of transient exposure to carbaryl wettable powder on the gut microbial community of honey bees. Applied Biological Chemistry, 62, 6. https://doi.org/10.1186/s13765-019-0415-7.   DOI
8 Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glockner FO (2013) The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Research, 41, D590-596. https://doi.org/10.1093/nar/gks1219.   DOI
9 Ritalahti KM, Amos BK, Sung Y, Wu Q, Koenigsberg SS, Loffler FE (2006) Quantitative PCR targeting 16S rRNA and teductive dehalogenase genes simultaneously monitors multiple Dehalococcoides strains. Applied and Environmental Microbiology, 72, 2765-2774. https://doi.org/10.1128/AEM.72.4.2765-2774.2006.   DOI
10 Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH et al. (2009) Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75, 7537-7541. https://doi.org/10.1128/aem.01541-09.   DOI
11 Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR et al. (2013) Ribosomal database project: Data and tools for high throughput rRNA analysis. Nucleic Acids Research, 42, D633-D642. https://doi.org/10.1093/nar/gkt1244.   DOI
12 Coursolle D, Gralnick J (2012) Reconstruction of extracellular respiratory pathways for iron(III) reduction in Shewanella oneidensis strain MR-1. Frontiers in Microbiology, 3, 56. https://doi.org/10.3389/fmicb.2012.00056.   DOI
13 Coursolle D, Gralnick JA (2010) Modularity of the Mtr respiratory pathway of Shewanella oneidensis strain MR-1. Molecular Microbiology, 77, 995-1008. https://doi.org/10.1111/j.1365-2958.2010.07266.x.   DOI
14 Jacoby R, Peukert M, Succurro A, Koprivova A, Kopriva S (2017) The role of soil microorganisms in plant mineral nutrition-Current knowledge and future directions. Frontiers in Plant Science, 8, 1617. https://doi.org/10.3389/fpls.2017.01617.   DOI
15 Pitts KE, Dobbin PS, Reyes-Ramirez F, Thomson AJ, Richardson DJ, Seward HE (2003) Characterization of the Shewanella oneidensis MR-1 decaheme cytochrome MtrA: Expression in Escherichia coli confers the ability to reduce soluble Fe(III) chelate. Journal of Biological Chemistry, 278, 27758-27765. https://doi.org/10.1074/jbc.M302582200.   DOI
16 Malla MA, Dubey A, Yadav S, Kumar A, Hashem A, Abd_Allah EF (2018) Understanding and designing the strategies for the microbe-mediated remediation of environmental contaminants using omics approaches. Frontiers in Microbiology, 9, 1132. https://doi.org/10.3389/fmicb.2018.01132.   DOI
17 Timmusk S, Behers L, Muthoni J, Muraya A, Aronsson A-C (2017) Perspectives and challenges of microbial application for crop improvement. Frontiers in Plant Science, 8, 49. https://doi.org/10.3389/fpls.2017.00049.   DOI
18 Wei S, Chelliah R, Park B-J, Kim S-H, Forghani F, Cho MS, Park D-S, Jin Y-G, Oh D-H (2019) Differentiation of Bacillus thuringiensis from Bacillus cereus group using a unique marker based on real-time PCR. Frontiers in Microbiology, 10, 883. https://doi.org/10.3389/fmicb.2019.00883.   DOI
19 Wilpiszeski R, Aufrecht J, Retterer S, Sullivan M, Graham D, Pierce E, Zablocki O, Palumbo A, Elias D (2019) Soil aggregate microbial communities: Towards understanding microbiome interactions at biologically relevant scales. Applied and Environmental Microbiology, 85, e00324-00319. https://doi.org/10.1128/AEM.00324-19.   DOI
20 Gupta A, Gupta R, Singh R (2017) Microbes and Environment. in: Singh R, Principles and applications of environmental biotechnology for a sustainable future. pp. 43-84, Springer, Singapore. ISBN: 978-981-10-1866-4.
21 Ahmad M, Pataczek L, Hilger TH, Zahir ZA, Hussain A, Rasche F, Schafleitner R, Solberg S (2018) Perspectives of microbial inoculation for sustainable development and environmental management. Frontiers in Microbiology, 9, 2992. https://doi.org/10.3389/fmicb.2018.02992.   DOI
22 Carvalho FP (2017) Pesticides, environment, and food safety. Food and Energy Security, 6, 48-60. https://doi.org/10.1002/fes3.108.   DOI
23 Wu F, Butz WP (2004) The future of genetically modified crops: Lessons from the green revolution, pp. 11-38, 1st edition, RAND Corporation, Santa Monica, CA.
24 Thakur M, Medintz IL, Walper SA (2019) Enzymatic bioremediation of organophosphate compounds-Progress and remaining challenges. Frontiers in Bioengineering and Biotechnology, 7, 289. https://doi.org/10.3389/fbioe.2019.00289.   DOI
25 M. Tahat M, M. Alananbeh K, A. Othman Y, I. Leskovar D (2020) Soil health and sustainable agriculture. Sustainability, 12, 4859. https://doi.org/10.3390/su12124859.   DOI
26 Ajiboye TO, Kuvarega AT, Onwudiwe DC (2020) Recent strategies for environmental remediation of organochlorine pesticides. Applied Sciences, 10, 6286. https://doi.org/10.3390/app10186286.   DOI
27 Liu L, Li W, Song W, Guo M (2018) Remediation techniques for heavy metal-contaminated soils: Principles and applicability. Science of The Total Environment, 633, 206-219. https://doi.org/10.1016/j.scitotenv.2018.03.161.   DOI
28 Miyashita NT (2015) Contrasting soil bacterial community structure between the phyla Acidobacteria and Proteobacteria in tropical Southeast Asian and temperate Japanese forests. Genes Genet Syst, 90, 61-77. https://doi.org/10.1266/ggs.90.61.   DOI
29 Yan W, Xiao Y, Yan W, Ding R, Wang S, Zhao F (2019) The effect of bioelectrochemical systems on antibiotics removal and antibiotic resistance genes: A review. Chemical Engineering Journal, 358, 1421-1437. https://doi.org/10.1016/j.cej.2018.10.128.   DOI
30 Trabelsi D, Mhamdi R (2013) Microbial inoculants and their impact on soil microbial communities: A review. BioMed Research International, 2013, 863240. https://doi.org/10.1155/2013/863240.   DOI