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http://dx.doi.org/10.5338/KJEA.2021.40.2.16

Development of Analytical methods for Chinomethionat in Livestock Products  

Yang, Seung-Hyun (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University)
Kim, Jeong-Han (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Choi, Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University)
Publication Information
Korean Journal of Environmental Agriculture / v.40, no.2, 2021 , pp. 134-141 More about this Journal
Abstract
BACKGROUND: The analytical method was established for determination of fungicide chinomethionat in several animal commodities using gas chromatography (GC) coupled with electron capture detector (ECD). METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining chinomethonat in various livestock products including beef, pork, chicken, milk and egg. Chinomethionat residual was extracted using acetone/dichloromethane(9/1, v/v) with magnesium sulfate and sodium chloride (salting outassociated liquid-liquid extraction). The extract was diluted by direct partitioning into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was finally purified with optimized silica gel 10 g. CONCLUSION: The method limit of quantitation (MLOQ) was 0.02 mg/kg, which was in accordance with the maximum residue level (MRL) of chinomathionate as 0.05 mg/kg in livestock product. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (84.8~103.0%). Reproducibilities were obtained (Coefficient of variation <5.2%), and the linearity of calibration curves were reasonable (r2>0.995) in the range of 0.01-0.2 ㎍/mL. This established analytical method was fully validated and could be useful for quantification of chinomathionat in animal commodities as official analytical method.
Keywords
Analytical Method; Chinomethionat; Livestock Product;
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1 Mastovska K, Lehotay SJ (2004) Evaluation of common organic solvents for gas chromatographic analysis and stability of multiclass pesticide residues. Journal of Chromatography A, 1040(2), 259-272. https://doi.org/10.1016/j.chroma.2004.04.017.   DOI
2 Vettorazzi, G. (1977). State of the art of the toxicological evaluation carried out by the joint FAO/WHO expert committee on pesticide residues. III. Miscellaneous pesticides used in agriculture and public health. Residue Reviews, 137-184. https://doi.org/10.1007/978-1-4612-6352-4_4.   DOI
3 Tseng S, Lin Y, Lee H, Su S, Chou S, Hwang D (2007) A multiresidue method for determining 136 pesticides and metabolites in fruits and vegetables: Application of macroporous diatomaceous earth column. Journal of Food and Drug Analysis, 15(3), 316-324.
4 Okihashi M, Kitagawa Y, Akutsu K, Obana H, Tanaka Y (2005) Rapid method for the determination of 180 pesticide residues in foods by gas chromatography/mass spectrometry and flame photometric detection. Journal of Pesticide Science, 30(4), 368-377. https://doi.org/10.1584/jpestics.30.368.   DOI
5 Kim YJ, Choi YH, Shin BW, Lee JH (2011) Comparison between the liquid-liquid partition method and modified QuEChERS method for the analysis of pesticide residues in beef fat. Korean Journal of Veterinary Service, 34(4), 429-439. https://doi.org/10.7853/kjvs.2011.34.4.429.   DOI
6 Hildmann F, Gottert C, Frenzel T, Kempe G, Speer K (2015) Pesticide residues in chicken eggs-A sample preparation methodology for analysis by gas and liquid chromatography/tandem mass spectrometry. Journal of Chromatography A, 1403, 1-20. https://doi.org/10.1016/j.chroma.2015.05.024.   DOI
7 Sturm J, Wienhold P, Frenzel T, Speer K (2018) Ultra turrax ® tube drive for the extraction of pesticides from egg and milk samples. Analytical and Bioanalytical Chemistry, 410, 5431-5438. https://doi.org/10.1007/s00216-018-1254-9.   DOI
8 Nutahara M, Murai T (1984) Accelerating effect of natural unsaturated fatty acids on photodecomposition of chinomethionat (Morestan ®). Journal of Pesticide Science, 9(4), 667-674. https://doi.org/10.1584/jpestics.9.667.   DOI
9 Chuang WC, Chen JW, Huang CH, Shyu TH, Lin SK (2019) FaPEx ® Multipesticide residues extraction kit for minimizing sample preparation time in agricultural produce. Journal of AOAC International, 102(6), 1864-1876. https://doi.org/10.1093/jaoac/102.6.1864.   DOI
10 Park JW, Kim AK, Kim JP, Lee HH, Park DW, Moon SJ, Ha DR, Kim ES, Seo KW (2014) Multi-residue analysis of pesticides using GC-TOF/MS, ECD, NPD with QuECHERS sample preparation. The Korean Journal of Pesticide Science, 18(4), 278-295. https://doi.org/10.7585/kjps.2014.18.4.278.   DOI
11 Katagi T (2004) Photodegradation of pesticides on plant and soil surfaces. Reviews of Environmental Contamination and Toxicology, 1-78. https://doi.org/10.1007/978-1-4419-9098-3_1.   DOI
12 Wu CC (2017). Multiresidue method for the determination of pesticides in Oolong tea using QuEChERS by gas chromatography-triple quadrupole tandem mass spectrometry. Food Chemistry, 229, 580-587. https://doi.org/10.1016/j.foodchem.2017.02.081.   DOI
13 Vargas-Perez M, Dominguez I, Gonzalez FJE, Frenich AG (2020) Application of full scan gas chromatography high resolution mass spectrometry data to quantify targeted-pesticide residues and to screen for additional substances of concern in fresh-food commodities. Journal of Chromatography A, 1622, 461118. https://doi.org/10.1016/j.chroma.2020.461118.   DOI