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http://dx.doi.org/10.5338/KJEA.2021.40.2.15

Development and Validation of Dithiocarbamates Fungicide Analytical Method using CS2 Trap Method in Livestock Product  

Jo, Hyeong-Wook (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation)
Sun, Jung-Hun (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation)
Heo, Hyo-Min (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation)
Lee, Sang-Hyeob (School of Applied Biosciences, Kyongbuk National University)
Kim, Jang-Eok (School of Applied Biosciences, Kyongbuk National University)
Moon, Joon-Kwan (School of Plant Resources and Landcape Architecture, Hankyong National University)
Publication Information
Korean Journal of Environmental Agriculture / v.40, no.2, 2021 , pp. 127-133 More about this Journal
Abstract
BACKGROUND: Dithiocarbamte fungicides have been used in crop cultivation for diseases protection and treatment. And cultivated agricultrual products were used as feedstuff, and residual pesticides are likely to be absorbed and transferred to livestock. But the maximum residue limits (MRLs) were not established for dithiocarbate fungicides in livestock products, and thus an analysis method was developed and validated for dithiocarbamate fungicides to establish MRLs. METHODS AND RESULTS: Samples were prepared using CS2 trap method and detected with UV/VIS spectrophotometer. Calibration line (0.1 ~ 10 ㎍/mL) was linear with r2 > 0.99. For validation, the recovery tests were carried out at three fortification levels (MLOQ, 10 MLOQ and 50 MLOQ) from livestock samples (egg, milk, beef, pork, and chicken). The results for mancozeb, propineb, and thiram ranged between 76.8 to 109.6%, 79.4 to 108.8%, and 80.2 to 107.8%, respectively and % RSD (relative standard deviation) values were below 9.5%. Furthermore, inter-laboratory analysis was performed to validate the method. CONCLUSION: All values were corresponded with the criteria ranges requested by both the CODEX (CAC/GL 40-1993, 2003) and MFDS guidelines (2016). This might be used as an official analytical method for determination of dithiocarbamate fungicides at established MRLs and monitoring.
Keywords
Livestock Product; $CS_2$ Trap Method; Dithiocarbamates; Fungicide; UV/VIS Spectrophotometer;
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