Browse > Article
http://dx.doi.org/10.5338/KJEA.2018.37.2.14

Determination of Oxycarboxin Residues in Agricultural Commodities Using HPLC-UVD/MS  

Choung, Myoung-Gun (Department of Herbal Medicine Resource, College of Health Science, Kangwon National University)
Publication Information
Korean Journal of Environmental Agriculture / v.37, no.2, 2018 , pp. 117-124 More about this Journal
Abstract
BACKGROUND: Oxycarboxin(5,6-dihydro-2-methyl-N-phenyl-1,4-oxathiin-3-carboxamide-4,4-dioxide) as oxanthiin is a systemic fungicide commonly used for control of various pathogens in agronomic and horticultural crops. In an effort to develop an analytical method to trace the fungicide, a method using HPLC equipped with UVD/MS was studied. METHODS AND RESULTS: Oxycarboxin was extracted with acetone from hulled rice, soybean, Kimchi cabbage, green pepper, and apple samples. The extract was diluted with saline water, followed by liquid-liquid extraction with methylene chloride. Florisil column chromatography was employed for the purification of the extracts. Oxycarboxin was determined on a Zorbax SB-AQ $C_{18}$ column by HPLC with UVD. Accuracy of the proposed method was validated by the recovery tests from crop samples fortified with oxycarboxin at 3 levels per crop. CONCLUSION: Mean recoveries ranged from 78.3% to 96.1% in five representative agricultural commodities. The coefficients of variation were less than 10%, and limit of quantitation of oxycarboxin was 0.04 mg/kg. A confirmatory technique using LC/MS with selected-ion monitoring was also provided to clearly identify the suspected residue. The method was reproducible and sensitive to determine the residue of oxycarboxin in agricultural commodities.
Keywords
Oxycarboxin; Oxanthiin fungicide; Pesticide residue;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Kwon, C. H., Chang, M. I., Im, M. H., Choi, H., Jung, D. I., Lee, S. C., Yu, J. Y., Lee, Y. D., Lee, J. O., & Hong, M. K. (2008). Determination of mandipropamid residues in agricultural commodities using high-performance liquid chromatography with mass spectrometry. Analytical Science and Technology, 21(6), 518-525.
2 Kulka, M., & Von Schmeling, B. (1995) Carboxin fungicides and related compounds : In Modern Selective Fungicides, pp. 133-147, 출판사명, New York. USA.
3 Lee, S. J., Hwang, Y. S., Kim, Y. H., Nam, M. Y., Hong, S. B., Yun, W. K., Kwon, C. H., Do, J. A., Im, M. H., Lee, Y. D., & Choung, M. G. (2010). Determination of fomesafen residue in agricultural commodities using HPLC-UVD/MS. The Korean Journal of Pesticide Science, 14(2), 95-103.
4 Miller, J. M. (2005). Chromatography: concepts and contrasts. pp. 286-287, John Wiley and Sons, New York, USA.
5 Paranjape, K., Vasant. G., & Sugha, G. (2014). Classification of pesticides, p. 342, The Pesticide Encyclopedia, CABI, UK.
6 Krieger, R. (Ed.). (2001). Handbook of pesticide toxicology, two-volume set: principles and agents (Vol. 2). pp. 1194-1198, Academic Press, USA.
7 Tomlin, C. D. S. (2003). The Pesticide Manual: a world compendium, Oxycarboxin. 13th ed. p. 1250, British Crop Protection Council, UK.
8 Ahn, K. G., Kim, G. H., Kim, G. P., Kim, M. J., Hwang, Y. S., Hong, S. B., Lee, Y. D., & Choung, M. G. (2014). Determination of amisulbrom residues in agricultural commodities using HPLC-UVD/MS. The Korean Journal of Pesticide Science, 18(4), 321-329.   DOI
9 Fong, W. G., Moye, H. A., Seiber, J. N., & Toth, J. P. (1999). Pesticide residues in foods: methods, techniques and regulations. pp. 3-44, John Wiley and Sons, New York, USA.
10 Hayat, K., Ashfaq, M., Ashfaq, U., & Saleem, M. A. (2010). Determination of pesticide residues in blood samples of villagers involved in pesticide application at District Vehari (Punjab), Pakistan. African Journal of Environmental Science and Technology, 4(10), 666-684.