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http://dx.doi.org/10.5338/KJEA.2017.36.2.14

Comparison of Flavonoid Characteristics between Blueberry (Vaccinium corymbosum) and Black Raspberry (Rubus coreanus) Cultivated in Korea using UPLC-DAD-QTOF/MS  

Jin, Young (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Kim, Heon-Woong (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Lee, Min-Ki (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Lee, Seon-Hye (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Jang, Hwan-Hee (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Hwang, Yu-Jin (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Choe, Jeong-Sook (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Lee, Sung-Hyun (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Cha, Youn-Soo (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University)
Kim, Jung-Bon (Department of Agro-food Resources, National Institute of Agricultural Sciences)
Publication Information
Korean Journal of Environmental Agriculture / v.36, no.2, 2017 , pp. 87-96 More about this Journal
Abstract
BACKGROUND: The objective of this study was to identify and compare the main phenolic compounds (anthocyanins, flavonoids, phenolic acids) in blueberry and black raspberry cultivated in Korea using ultra-performance liquid chromatography diode array detection-quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS). METHODS AND RESULTS: Twenty-nine flavonoids were identified by comparison of ultraviolet and mass spectra with data in a chemical library and published data. Blueberry contained flavonols including kaempferol, quercetin, isorhamnetin, myricetin, and syringetin aglycones. Isorhamnetin 3-O-robinobioside, kaempferol 3-O-(6"-O-acetyl)glucoside, quercetin, quercetin 3-O-arabinofuranoside (avicularin), quercetin 3-O-(6''-O-malonyl) glucoside, and quercetin 3-O-robinobioside were detected for the first time in blueberry. The flavonoids in raspberry consisted of quercetin aglycone and its glycosides. The mean total flavonoid content in blueberry [143.0 mg/100 g dry weight (DW)] was 1.5-times that in raspberry (95.4 mg/100 g DW). The most abundant flavonoid in blueberry was quercetin 3-O-galactoside (hyperoside, up to 76.1 mg/100 g DW) and that in raspberry was quercetin 3-O-glucuronide (miquelianin, up to 55.5 mg/100 g DW). Miquelianin was not detected in blueberry. CONCLUSION: Flavonol glycosides were the main flavonoids in blueberry and black raspberry cultivated in Korea. The composition and contents of flavonoids differed between blueberry and black raspberry, and may be affected by the cultivar and cultivation conditions.
Keywords
Flavonoid; Rubus coreanus; UPLC-DAD-QTOF/MS; Vaccinium corymbosum;
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Times Cited By KSCI : 12  (Citation Analysis)
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