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http://dx.doi.org/10.5338/KJEA.2013.32.3.240

Selective Isolation and Phylogeny of the Yeast Species Associated with Aloe vera and Aloe saponaria  

Choi, Sungchang (Gyeongbuk Institute for Marine Bio-Industry)
Kim, Myung-Uk (Gyeongbuk Institute for Marine Bio-Industry)
Kim, Jong-Shik (Gyeongbuk Institute for Marine Bio-Industry)
Publication Information
Korean Journal of Environmental Agriculture / v.32, no.3, 2013 , pp. 240-243 More about this Journal
Abstract
BACKGROUND: Several yeast species have potential applications in biotechnology and the identification of such yeast species is of great interest. The first step in the identification of yeasts is the establishment of an effective isolation method. Thus, we compared the efficacy of different yeast media in the isolation of yeast associated with Aloe vera and Aloe saponaria. METHODS AND RESULTS: In this study, we spread homogenized A. vera and A. saponaria leaves onto 4 different yeast selective media containing chloramphenicol, streptomycin, Triton X-100 and L-sorbose. We observed high selectivity for yeast and many colonies on media. We isolated 67 yeast strains from A. vera and 42 yeast strains from A. saponaria. We used phylogenetic analysis to identify the yeast isolates based on ITS region sequencing and performed sequence analysis on representative isolates from each agar plate. Further, we compared the sequences obtained with reference sequences. The yeast species isolated from A. vera were as follows: 56 isolates of Meyerozyma, 9 isolates of Cryptococcus, and 1 isolate each of Rhodotorula and Sporobolomyces. Those isolated from A. saponaria were as follows: 41 isolates of Rhodosporidium and 1 isolate of Sporobolomyces. CONCLUSION(S): All the isolates obtained using large agar plate containing chloramphenicol, streptomycin, Triton X-100 and L-sorbose were identified as yeast. Therefore, we concluded that this method is useful for selective screening of yeast species.
Keywords
Aloe saponaria; Aloe vera; ITS gene; Yeast screening method;
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1 White, T.J, Bruns, T., Lee, S., Taylor, J., 1990. Amplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J., (Eds), PCR protocols: a guide to methods and applications, Academic press, San Diego, pp. 315-322.
2 Yagi, A., Shibata, S.J., Nishioka, I., Iwadare, S., Ishida, Y., 1982. Cardiac stimulant action of constituents of Aloe saponaria, J. Pharm. Sci. 71, 739-741.   DOI
3 Kluge, M., Knapp, I., Kramer, D., Schwerdtner, I., Ritter, H., 1979. Crassulacean acid metabolism (CAM) in leaves of Aloe arborescens Mill comparative studies of the carbon metabolism of chlorenchym and central hydrenchym, Planta 145, 357-363.   DOI   ScienceOn
4 Raspor, P., Zupan, J., 2006. Yeast in extreme environments, in: Rosa, C.A., Peter, G. (eds), Biodiversity and Ecophysiology of Yeasts, Springer, Berlin, pp.370-417.
5 Botha, A., 2011. The importance and ecology of yeasts in soil, Soil Biol. Biochem. 43, 1-8.   DOI   ScienceOn
6 Deak, T., 2009. Ecology and biodiversity of yeasts with potential value in biotechnology, in: Satyanarayana, T. Kunze, G. (Eds), Yeast Biotechnology: Diversity and Applications, Springer Science + Business Media B.V., Dordrecht, pp. 151-168.
7 Reynolds, T., 2004. Aloes : the genus aloe, CRC press. p. 408.
8 Reynolds, T., Dweck, A.C., 1999. Aloe vera leaf gel: A review update. J. Ethnopharmacol. 68, 3-37.   DOI   ScienceOn
9 Rodriguez, E.R., Martin, J.D., Romero, C.D., 2010. Aloe vera as a functional ingredient in foods, Crit. Rev. Food. Sci. 50, 305-326.   DOI   ScienceOn
10 Saitou, N., Nei, M., (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol. 4, 406-425.
11 Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol. 28, 2731-2739.   DOI   ScienceOn
12 Sampedroa, M.C., Artolab, R.L., Muraturec, M., Nuratureb, D., Ditamoa, Y., Rotha, G.A., Kivatinitza, S., 2004. Mannan from Aloe saponaria inhibits tumoral cell activation and proliferation, Int. Immunopharm. 4, 411-418.   DOI   ScienceOn
13 Tamang, J.P., Fleet, G.H., 2009. Yeasts diversity in fermented foods and beverages, in: Satyanarayana, T., Kunze G. (Eds), Yeast Biotechnology: Diversity and Applications, Springer Science + Business Media B.V., Dordrecht, pp. 169-198.
14 Grindlay, D., Reynolds, T., 1986. The Aloe vera phenomenon: A review of the properties and modern uses of the leaf parenchyma gel, J. Ethnopharmacol. 16, 117-151.   DOI   ScienceOn