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http://dx.doi.org/10.5338/KJEA.2011.30.1.76

Repellent Activity of the Extracts of Acorus Gramineus against Mosquito (Culex pipinens pallens)  

Lee, Seung-Ho (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
Jung, Jin-Kwan (Youngil High School)
Park, Sang-Hyun (Youngil High School)
Kim, Mun-Sik (Youngil High School)
Han, Yeon-Soo (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
Seo, Jung-Mi (Gwangju Advanced Institute of Health and Environment)
Kim, In-Seon (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
Publication Information
Korean Journal of Environmental Agriculture / v.30, no.1, 2011 , pp. 76-81 More about this Journal
Abstract
BACKGROUND: Acorus gramineus is of medicinal plants that exhibit variable biological activities for human health and against insect pests. The extracts of A. gramineus was examined in an attempt to develop a natural repellent against human disease-mediating Culex pipiens. METHODS AND RESULTS: The roots of A. gramineus dried under dark conditions were homogenized and extracted with ethanol. The extracts were subjected to repellent activity assays against C. pipiens in a hand-made acrylamide box with three accessible rooms. Significantly low number of mosquitos was found in the room previously fumigated with the extracts at 50 mg/L on the filter paper, exhibiting less than 20% of mosquitos tested. More than 50% of mosquitos tested was found in the room without the extracts, but less than 30% was found in the room that released mosquitos. GC/MS analysis detected ${\beta}$-asarone as a main component of the extracts. The commercial asarones (${\alpha}$ and ${\beta}$) showed a repellent activity at 50 mg/L on the filter paper similar to the extracts. CONCLUSION(S): A. gramineus has potential for use as a mosquito repellent since ${\beta}$-asarone, a main component of the plant, exhibited a strong repellent activity against C. pipiens.
Keywords
Acorus gramineus; Asarone; Medicinal plant; Mosquito; Repellent;
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