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http://dx.doi.org/10.5338/KJEA.2011.30.1.60

Application of Macroporous Diatomaceous Earth Column for Residue Analysis of Insecticide Endosulfan in Herbal Medicines  

Hwang, Jeong-In (School of Applied Biosciences, Kyungpook National University)
Jeon, Young-Hwan (School of Applied Biosciences, Kyungpook National University)
Kim, Hyo-Young (School of Applied Biosciences, Kyungpook National University)
Kim, Ji-Hwan (School of Applied Biosciences, Kyungpook National University)
Lee, Yoon-Jeong (Division of Herbal Medicine Research, Korea Food and Drug Administration)
Park, Ju-Young (Division of Herbal Medicine Research, Korea Food and Drug Administration)
Kim, Do-Hoon (Division of Herbal Medicine Research, Korea Food and Drug Administration)
Kim, Jang-Eok (School of Applied Biosciences, Kyungpook National University)
Publication Information
Korean Journal of Environmental Agriculture / v.30, no.1, 2011 , pp. 60-67 More about this Journal
Abstract
BACKGROUND: Because dried herbal medicines have many active ingredients, it is not easy to determine the residue amount after extraction, partition and clean up of pesticides from them. Especially, liquid-liquid partition method is consuming many times and solvents. Macroporous diatomaceous earth(MDE) column was used to replace the separatory funnel for liquid-liquid extraction with dichloromethane to analyze the endosulfan and its metabolite. METHODS AND RESULTS: The residue analysis method using MDE column instead of liquid-liquid partition for determining insecticide endosulfan and its metabolite in 4 dried herbal medicines was developed by GC/MS. As a result, the recovery rates of the pesticides in 4 herbal medicines were ranged from 80.3 to 93.5% for ${\alpha}$-endosulfan, from 81.0 to 100.3% for ${\beta}$-endosulfan and from 80.6 to 95.6% for endosulfan sulfate, respectively. The coefficients of variation for triplicate were ranged from 1.1 to 3.4%. CONCLUSION: The improved methods are more ecofriendly, safer, faster and less laborious than conventional method by KFDA.
Keywords
Multiresidue analysis method; MDE column; Herbal medicine; Endosulfan; GC/MS;
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