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http://dx.doi.org/10.5338/KJEA.2010.29.4.374

Effects of Light Quality and Lighting Type Using an LED Chamber System on Chrysanthemum Growth and Development Cultured In Vitro  

Heo, Jeong-Wook (Climate Change & Agroeconomy Division, Department of Agricultural Environment, National Academy of Agricultural Science, RDA)
Lee, Yong-Beom (Research Policy Bureau, On-Farm Research Division, Rural Development Administration)
Chang, Yu-Seob (Farming Automation Division, Department of Agricultural Engineering, National Academy of Agricultural Science, RDA)
Lee, Jeong-Taek (Climate Change & Agroeconomy Division, Department of Agricultural Environment, National Academy of Agricultural Science, RDA)
Lee, Deog-Bae (Climate Change & Agroeconomy Division, Department of Agricultural Environment, National Academy of Agricultural Science, RDA)
Publication Information
Korean Journal of Environmental Agriculture / v.29, no.4, 2010 , pp. 374-380 More about this Journal
Abstract
This experiment was carried out to investigate the effect of light qualities and lighting types provided by LED Chamber System which designed by Rural Development Administration on growth and development of Chrysanthemum (Dendranthema grandiflorum L., cv. 'Cheonsu') plantlet cultured in vitro. The explants of single-node cuttings were exposed to monochromic or mixture radiation of blue, red, or green under continuous and intermittent lighting for 42 days. The intermittent lighting of 20 sec. on and off per minute significantly stimulated shoot elongation with lower number of internodes compared with continuous lighting treatments. However, continuous blue, red, or green light gave greater dry weight comparing the intermittent lighting, and the lowest weight was recorded at the continuous fluorescent lamp. Otherwise, the plantlet growth in dry weight or leaf area was inhibited by the green light controlled at 50 times intermittence but internode elongation was significantly increased. These results showed that the plantlets were successfully grown under the LED Chamber System controlled with different light qualities and lighting types. Quantitative growth of the plantlets was improved under the shorter photoperiod with a intermittent lighting cycle compared with continuous lighting using fluorescent lamps. It is concluded that the growth and development of in vitro plantlets such as single-node cuttings can be achieved by the controlling of light quality or lighting type during the photoperiod per day with a lower electric cost compared with conventional continuous lighting system.
Keywords
Continuous Radiation; Light-Emitting Diodes (LEDs); Light quality; Photoperiod; Single-node cuttings;
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1 Kozai, T., Watanabe, K., Jeong, B.R., 1995. Stem elongation and growth of Solanum tuberosum L. in vitro in response to photosynthetic photon flus, photoperiod and difference in photoperiod and dark period temperatures. Sci. Hort. 64(1), 1-9.   DOI   ScienceOn
2 Kurilcik, A., Miklusyte-Canova, R., Dapkuniene, S., Zilinskaite S., Kurilcik, G., Tamulaitis, G., Duchovskis, P., Zukauskas, A., 2008. In vitro culture of chrysanthemum plantlets using light-emitting diodes. Central Euro. J. Biol. 3(2), 161-167.   DOI
3 Lee, B.J., Won, M.K., Choi, T.Y., Kim, K.W., Lee, J.S., 2005. Spectral properties of sunlight and phytochrome photoequilibrium as influenced by leaf number in chrysanthemum. J. Korean Hort. Sci. 46(2), 161-167.   과학기술학회마을
4 Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473-497.   DOI
5 Oyaert, E., Volckaert, E., Debergh, P.C., 1999. Growth a chrysanthemum under coloured plastic films with different light qualities and quantities. Sci. Hort. 79, 195-205.   DOI   ScienceOn
6 Saebo, A., Krekling, T., Appelgren, M., 2000. Influence of light quality on in vitro photosynthesis, leaf morphometry, leaf anatomy and field performance in micropropagated Betula pendula Rooth. Acta Hort. Abst. No. 327.
7 Schuerger, A.C., Brown, C.S., 1994. Spectral quality may be used to alter plant disease development in CELSS. Adv. Space Res. 14, 395-398.
8 Sivakumar, G., Heo, J.W., Kozai, T., Paek, K.Y., 2006. Effect of continuous or intermittent radiation on sweet potato plantlets in vitro. J. Hort. Sci. & Biotech. 81(3), 546-548.   DOI
9 Sun, J., Nishio, J.N., Vogelmann, T.C., 1998. Green light drives $CO_{2}$ fixation deep within leaves. Plant Cell Physiol. 39(10), 1020-1026.   DOI
10 Gepstein, S., Thimann, K.V., 1980. The effect of light on the production of ethylene from 1-aminocyclopropane- 1-carboxylic acid by leaves. Planta. 149, 196-199.   DOI   ScienceOn
11 Heo, J.W., Lee, C.W., Chakrabarty, D., Paek, K.Y., 2002. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regul. 38, 225-230.   DOI   ScienceOn
12 Heo, J.W., Lee, C.W., Murthy, H.N., Paek, K.Y., 2003. Influence of light quality and photoperiod on flowering of Cyclamen persicum Mill. cv ‘Dixie White’. Plant Growth Regul. 40, 7-10.   DOI   ScienceOn
13 Khattak, A.M. Pearson, S., 2005. Light quality and temperature effects on antirrhinum growth and development. J. Zhejiang Univ. Sci. B. 6(2), 119-124.
14 Heo, J.W., Shin, K.S., Kim, S.K., Paek, K.Y., 2006. Light quality affects in vitro growth of grape ‘Teleki 5BB’. J. Plant Biol. 49, 276-280.   DOI   ScienceOn
15 Heo, .JW., Lee, Y.B., Lee, D.B., Chun, C., 2009. Light quality affects growth, net photosynthetic rate, and ethylene production Ageratum, African Marigold, and Salvia seedlings. J. Korean Hort. Sci. & Tech. 27(2), 187-193.   과학기술학회마을
16 Heo, .JW., Lee, Y.B., Kim, D.E., Chang, Y.S., Chun. C., 2010. Effects of supplementary LED lighting on growth and biochemical parameters in Dieffenbachia amoena 'Camella' and Ficus elastica 'Melany'. J. Korean Hort. Sci. & Tech. 28(1), 51-58.
17 Khattak, A.M., Pearson, S., Johnson, C.B., 2004. The effects of far red spectral filters and plant density on the growth and development of chrysanthemum. Sci. Hort. 102, 335-341.   DOI   ScienceOn
18 Kim, S.J., Hahn, E.J., Heo, J.W., Paek K.Y., 2004. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci. Hort. 101, 143-151.   DOI   ScienceOn
19 Appelgren, M., 1991. Effect of light quality on stem elongation of Pelargonium. Sci. Hort. 45, 345-406.   DOI   ScienceOn
20 Adams, S.R., Langton, F.A., 2005. Photoperiod and plant growth. J. Hort. Sci. & Biotech. 80(1), 2-10.   DOI
21 Bula, R.J., Morrow, T.W., Tibbitts, T.W., Barta, D.J., Ignatius, R.W., Martin, T.S., 1991. Light-emitting diodes as a radiation source for plants. HortSci. 120, 808-813.
22 Casal, J.J., 2000. Phytochromes, Cryptochromes, Phototropin: Photoreceptor interactions in plants. PhotoChem. PhotoBiol. 71(1), 1-11.   DOI   ScienceOn
23 Folta, K.M., 2004. Green light stimulates early stem elongation, antagonizing light-mediated growth inhibition. Plant Physiol. 135, 1407-1416.   DOI   ScienceOn
24 Tennessen, D.J., Singrass, E.L., Sharkey, T.D., 1994. Light emitting diodes as a light source for photosynthesis research. Photosynthesis Res. 39, 85-92.   DOI   ScienceOn