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http://dx.doi.org/10.5338/KJEA.2010.29.2.176

Biodegradation of Endosulfan by Klebsiella oxytoca KE-8 Immobilized on Activated Carbon  

Jo, Min-Sub (School of Bioresource Sciences, Andong National University)
Lee, Jung-Bok (Dept. of Food & Nutrition Science, Kundong University)
Kim, Jang-Eok (School of Biosciences, Kyungpook National University)
Sohn, Ho-Yong (Dept. of Food & Nutrition, Andong National University)
Jeon, Chun-Pyo (School of Bioresource Sciences, Andong National University)
Choi, Chung-Sig (HansBio Co. Research Center)
Kwon, Gi-Seok (School of Bioresource Sciences, Andong National University)
Publication Information
Korean Journal of Environmental Agriculture / v.29, no.2, 2010 , pp. 176-183 More about this Journal
Abstract
Endosulfan degrading ability of Klebsiella oxytoca KE-8 immobilized by entrapment with activated carbon was examined. Endosulfan degradation by the immobilized bacterial strains on several different activated carbon based support materials was investigated. Based on results, activated carbon ($8\times30$ mesh) was chosen as a support material. The immobilized Klebsiella oxytoca KE-8 with the cell density of 4 mg $g^{-1}$ (dry weight) degraded 22.18 ug $ml^{-1}$ endosulfan within 5 days at pH 7.0, $30^{\circ}C$ in batch shake flask cultures. Also, we an experimented recycle packed bed column mode and continuous packed bed column mode for endosulfan degradation. Under optimum operation condition, the immobilized cells in a laboratory scale pack bed column with support beads were able to degrade endosulfan completely in defined minimal salt medium at a maximum rate of 129.6 ug $ml^{-1}$ per day. Moreover, the endosulfan degradation activity could be demonstrated at $4^{\circ}C$ for one month without significant decrease in activity. Results of this study suggest that immobilized cells of Klebsiella oxytoca KE-8 might be applicable to endosulfan contaminated site.
Keywords
Biodegradation; Detoxification; Endosulfan; Immobilization; Klebsiella oxytoca;
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