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http://dx.doi.org/10.5338/KJEA.2009.28.4.462

Apoptotic effect of formaldehyde in cultured human hepatocyte cell lines  

Park, Soo-Hyun (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Publication Information
Korean Journal of Environmental Agriculture / v.28, no.4, 2009 , pp. 462-467 More about this Journal
Abstract
Exposure of formaldehyde (FA), one of the major compounds in pesticides and in the onset of sick house syndrome, has been implicated in the development of diverse diseases. Liver is a very important organ to body metabolism and drug detoxification. Apotosis of hepatocytes is associated with the onset of liver diseases such as hepatitis. However, the apoptotic effect of FA in hepatocytes is not clear. Therefore, this study was conducted to investigate the effect of FA on the apoptosis in HepG2 cells, a human hepatocyte cell line. As a result, FA (> $500\;{\mu}M$) decreased cell viability and increased lactate dehydrogenase activity in HepG2 cells, which was blocked by the treatment of vitamin E and N-acetylcysteine (NAC). In addition, FA decreased glutathione (GSH) contents and Bcl-2 levels, while increasing lipid peroxide formation and Bax levels. It also cleaved caspase-3 form, which was blocked by the treatment of vitamin E and NAC. It is insisted that FA induced apoptosis via oxidative stress in human hepatocytes.
Keywords
Apoptosis; Formaldehyde; Human hepatocytes; Oxidative stress;
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Times Cited By KSCI : 1  (Citation Analysis)
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