Browse > Article
http://dx.doi.org/10.5338/KJEA.2009.28.3.266

Simultaneous Determination of Tetracyclines and Sulfonamides Residues in Pork and Flatfish Using Matrix Solid Phase Dispersion (MSPD) Extraction and HPLC  

Gil, Geun-Hwan (National Academy of Agricultural Science, RDA)
Ko, Kwang-Yong (Dept. of Agriculture Chemistry, Chungnam National University)
Lee, Yong-Jae (Central Research Institute, KT&G)
Park, Hye-Jin (Food Science Department South Dakota State University)
Lee, Kyu-Seung (Dept. of Agriculture Chemistry, Chungnam National University)
Publication Information
Korean Journal of Environmental Agriculture / v.28, no.3, 2009 , pp. 266-273 More about this Journal
Abstract
This study was conducted to develop more convenient simultaneous determination method by matrix solid phase dispersion (MSPD) and HPLC for sulfonamides such as sulfamerazine (SMR), sulfamethazine (SMT), sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfaquinoxaline (SQX), and tetracyclines including oxytetracycline (OTC), tetracycline (TC) chlortetracycline (CTC) in prok and flatfish. The limits of detection were 0.047 $mg{\cdot}kg^{-1}$ for OTC, TC, SMR, SMT and SMM, and 0.033 $mg{\cdot}kg^{-1}$ for CTC, SDM, and SQX, respectively. So it is sufficiently possible to detect the eight tetracyclines and sulfonamides under their MRLs ($0.1{\sim}0.2mg{\cdot}kg^{-1}$). The average percentage recoveries of sulfonamides and tetracyclines from pig muscle and flatfish spiked standard solution were approximately $80.25{\sim}101.25%$ and $85.77{\sim}121.42%$, respectively. Therefore this method was efficient for simultaneous analysis of eight tetracyclines and sulfonamides.
Keywords
tetracyclines; sulfonamides; pig muscle; flatfish; MSPD; HPLC;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Heo, G. J., Shin, K. S. and Lee, M. H. (1992) Diseases of aquaculture animals and prevention of drug residues, Kor. J. Food Hygiene 7(2, 3), 7-9
2 Hah, D. S., Kim, J. S. and Kim, G. S. (1997) Simultaneous quantification of sulfonamide and tetracyclines in fish muscle tissue by matrix solid phase dispersion (MSPD) extraction and HPLC, J. Fd. Hyg. safety 12(2), 117-124
3 KFDA (2008), Korea food code, Attached table 7.1-7.19, pp. 807-809
4 Kang, H. G., Son, S. W., Cho, B. H., Lee, H. S., Park, S. J., Kim, J. H. and Cho, M. H. (1996) Multiresidue matrix solid phase dispersion (MSPD) extraction and HPLC determination of tetracyclines in animal muscle tissue, Korean J. Vet. Res. 36(3), 541-550
5 Baker, S. A. and Walker, C. C. (1992) Chromatographic methods for tetracycline analysis in food, J. Chrom. 624, 195-209   DOI   ScienceOn
6 Lu, H. T. and Jiang, Y. (2004) Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high performance liquid chromatography with fluorescence detection, Chromatographia 60(5-6), 259-264
7 Long, A. R., Hsieh, L. C. and Baker S. A. (1990) Matrix solid phase dispersion isolation and liquid chromatographic determination of oxytetracycline in catfish muscle tissue, J. Assoc. Off. Anal. Chem. 73, 864-867
8 Reimer, G. J. and Suarez, A. (1992) Liquid chromatographic confirmatory method for five sulfonamides in salmon muscle tissue by matrix solid-phase dispersion, J. Assoc. Off. Anal. Chem. 75(6), 979-981
9 Baker, S. A. and Long, A. R. (1994) Preparation of milk sample for immunoassay and liquid chromatographic screening using MSPD, J. Assoc. Off. Anal. Chem. 77, 848-854
10 Booth, N. H. and Mcdonald, L. E. (1988) Drug and chemical residues in edible tissues of animals : Verterinary pharmacology and therapeutics 6th ed., Iowa State University, Iowa, United States of Ametica, pp. 1149-1201
11 Park, J. J., Lee, K. C. and Koh, H. B. (1994) A study on the sulfonamide residues in bovin, swine, chicken by high performance liquid chromatography, Kor. J. Vet. Publ. Health. 18(4), 317-326
12 Baker, S. A., Long, A. R. and Short, C. R. (1989) Isolation of drug residues from tissues by solid phase dispersion, J. Chrom. 475, 353-36   DOI   ScienceOn
13 Austin, R. L. (1991) Matrix solid phase dispersion and gas chromatographic screening of nine chlorinated pesticides in beef fat, J. Assoc. Off. Anal. Chem. 74(3), 493-496
14 Jung, K. S., Chae, M. S., Kim, C. D. and Kim, J. B. (1993) Simultaneous determination of residual synthetic antimicrobials in animal muscles by high performance liquid chromatography, Kor. J. Food Hygiene 8(1), 25-35
15 Smedley M. D. and Weber J. D. (1990) Liquid chromatographic determination of multiple sulfonamide residues in bovine milk, J. Assoc. Off. Anal. Chem. 73(6), 875-879
16 Onji, Y., Uno, M. and Tanigawa, K. (1984) Liquid chromatographic determination of tetracycline residues in meat and fish, J. Assoc. Off. Anal. Chem. 67(6), 1135-1137
17 Oka, H., Ikai, Y. and Hayakawa, J. (1994) Improvement of chemical analysis of antibiotics, part XIX, chromatography and thin layer chromatography / fast atom bombardment mass spectrophotometry, J. Agri. Food Chem. 42, 615-622
18 AOAC 18th edition (2005), Official methods of analysis of AOAC international Vol. 1, Chapter 23, pp. 18-19
19 Horie, M., Saito, H., Hosinno, Y., Nose, H., Nakajawa, H. and Yamane, Y. (1991) Simultaneous determination of residual synthetic antibacterials in fish by high performance liquid chromatography, J. Chrom. 538, 484-491   DOI   ScienceOn
20 Long, A. R., Hsieh, L .C., Malbrough, M. S., Short, C. R. and Baker, S. A. (1990) Multiresidue method for the determination of sulfonamides in pork tissue, J. Agric. Food Chem. 38(2), 423-426   DOI
21 Bevill, R. F., Sharma, R. M., Meachum, S. H., Wozniak, S. C., Bourne, D. W. A. and Ditert, L. W. (1977) Disposition of sulfonamides in food producing animals : Concentrations of sulfamethazine and its metabolites in plasma, urine and tissues of lambs following intravenous administration, Am. J. Vet. Res. 38, 973-977
22 Blood, D. C., Henderson, J. A. and Radostitis, O. M. (1979) Verterinary medicine 5th ed., Cassel Ltd., London, U.K., pp. 87-90
23 Horwits, W. (1981) Review of analytical method for sulfonamides analytical methods for sulfonamides in food and feeds, J. Assoc. Off. Anal. Chem. 64(1), 104-130
24 Tsai, C. E. and Kondo, F. (1994) Simple continuous and simultaneous determination of tetracycline residue, Research in Vet. Science 56, 277-283   DOI   ScienceOn
25 Ryu, J. C., Song, Y. S., Park, J. S., Chang, J. S. and Jinbo, K. (1993) A study on the determination of residual antibiotics and synthetic antibacterial agents in meat (1)- microbiological assay, Kor. J. Food Hygiene 8(1), 1-8
26 Yoshimura, H., Osawa, N. and Sugimori, T. (1991) Residues of doxycycline and oxytetracycline in eggs after medication via drinking water to laying hen, Food Addit. Contam. 8, 65-69   DOI   ScienceOn
27 Long, A. R., Hsieh, L. C., Malbrough, M. S., Short C. R. and Baker, S. A. (1990) Matrix solid phase dispersion (MSPD) isolation and liquid chromatographic determination of oxytetracycline, tetracycline, chlorotetracycline in milk, J. Assoc. Off. Ana. Chem. 73(3), 379-384
28 Park, J. M., Namgoong, S., Lee, M. H., Cho, T. H., Lee, K. J. and Jin, N. S. (1993) Simultaneous determination of tetracyclines in beef, pork and chicken by high performance liquid chromatography using matrix solid phase dispersion extraction, Kor. J. Vet. Publ. Health. 17(3), 321-328