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http://dx.doi.org/10.5338/KJEA.2007.26.3.239

Effects of Ultraviolet-B Radiation on Photosynthesis in Tobacco (Nicotiana tabacum cv. Petit Havana SR1) Leaves  

Lee, Hae-Youn (The Research Institute of Basic Sciences, Seoul National University)
Park, Youn-Il (Chungnam National University)
Hong, Young-Nam (Seoul National University)
Publication Information
Korean Journal of Environmental Agriculture / v.26, no.3, 2007 , pp. 239-245 More about this Journal
Abstract
The effect of ultraviolet-B (UV-B) radiation on photosynthesis was studied by the simultaneous measurements of $O_2$ evolution and chlorophyll (Chl) fluorescence in tobacco leaves. When the tobacco leaves were teated with UV-B (1 $W{\cdot}m^{-2}$), the maximal photosynthetic $O_2$, evolution (Pmax; 4.60 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) at 200 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) was decreased with increasing time of UV-B treatment showing 80% decline after 4 h treatment. Chl fluorescence parameters were also affected by ultraviolet-B. Fo was increased while both Fm and Fv were decreased, resulted in the decreased of photochemical efficiency of PSII (Fv/Fm). Non-radiative dissipation of absorbed light as heat as estimated as NPQ (Fm/Fm' - 1) was also decreased with increasing time of UV-B treatment while the extent of photochemical quenching (qP) was not changed. Thus, the ratio of (1-qP)/NPQ parameter was also increased with increasing time of UV-B treatment indicating PSII is under the threat of photoinhibition. The result indicate that UV-B primarily decreases the capacity to dissipate excitation energy by trans-thylakoid pH, which in turn inhibits PSII activity.
Keywords
Ultraviolet-B Radiation; photosynthesis; Chlorophyll fluorescence; Photosystem II; Photochemical quenching (qP); Non-photochemical quenching (NPQ);
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