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http://dx.doi.org/10.5338/KJEA.2006.25.1.040

Inhibitory Effect of the Selected Heavy Metals on the Growth of the Phosphorus Accumulating Microorganism, Acinetobacter sp.  

Chung, Keun-Yook (Department of Agricultural Chemistry, Chungbuk National University)
Han, Seok-Soon (Department of Agricultural Chemistry, Chungbuk National University)
Kim, Hong-Ki (Department of Agricultural Chemistry, Chungbuk National University)
Choi, Guak-Soon (Department of Agricultural Chemistry, Chungbuk National University)
Kim, In-Su (Department of Agricultural Chemistry, Chungbuk National University)
Lee, Sang-Sung (Department of Agricultural Chemistry, Chungbuk National University)
Woo, Sun-Hee (Department of Crop Science, Chungbuk National University)
Lee, Kyung-Ho (Department of Environmental Planning, Gyeongju University)
Kim, Jai-Joung (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Environmental Agriculture / v.25, no.1, 2006 , pp. 40-46 More about this Journal
Abstract
This study was initiated to evaluate the inhibitory effect of selected heavy metals on the growth of Acinetobacter sp. Down as one of the phosphorus accumulating microorganisms (PAO) involved in the enhanced biological phosphorus removal (EBPR) process of the wastewater treatment plant. Acinetobacter sp. was initially selected as a starting model microorganism and was grown under aerobic condition for this experiment. The heavy metals selected and investigated in this study were cadmium (Cd), copper (Cu), mercury (Hg), nickel (Ni), and zinc (Zn). Median $(IC_{50})$ and threshold $(IC_{10})$ inhibitory concentrations for Cd, Cu, Hg, Ni, and Zn were 2.95 and 1.45, 4.92 and 2.53, 0.03 and 0.02, 1.12 and 0.43, 14.84 and 5.46 mg $L^{-1}$, respectively. We demonstrated that most of heavy metals tested in the experiment inhibited the growth of Acinetobacter sp. in the range of predetermined concentrations. Based on the data obtained from the experiment, Hg was the most sensitive to Acinetobacter sp., then Ni, Cd, Cu, and Zn in order.
Keywords
Inhibition; growth; heavy metals; Acinetobacter sp.; PAO;
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1 Schmitz, R., Eisentrager, A., and Dott, W. (1998) Miniaturized kinetic growth inhibition assays with Vibrio jischeri and Pseudomonas putida. J. Microbiol. Methods 31, 159-166   DOI   ScienceOn
2 Sauvant, M.P., Pepin, D., Bohatier, J., and Groliere, C.A. (1995) Microplate technique for screening and assessing cytotoxicity of xenebiotles with Tetrahyrrena pyriformis. Ecotoxicol. Environ. Saf. 32, 159- 165   DOI   ScienceOn
3 Hoffman, D.J., Rattner, B.A., Burton, G.A., and Cairns, J. (1995) Handbook of ecotoxicology. Lewis Publishers. London
4 Slabbert, J.L. (1986) Improved bacterial growth test for rapid water toxicity screening. Bull. Environ. Contam. Toxicol. 37, 56-569
5 Schmitt, M, Gellert G, Ludwig, J, and Lichtenberg-Frate H. (2004) Phenotypic yeast growth analysis for chronic toxicity testing. Ecotoxicol. Environ. Saf. 59, 142-150   DOI   ScienceOn
6 Reinke, M., Kalinowski, G., and Dott, W. (1995) Evaluation of an automated, miniaturized Pseudomonas putida growth inhibition assay. Vom Wasser 85, 199-213
7 Paran, J.H., Sharma, S., and Quershi, A.A. (1990) A rapid and simple toxicity assay based on growth rate inhibition of Pseudomonas fluorescens. Toxic. Assess. 5, 351-365   DOI
8 Cho, K.S., Koo, S.Y., Kim, J.Y., and Ryu, H.W. (2004) Quantification of inhibitory impact of heavy metals on the growth of Escherichia coli. Kor. J. Microbiol. Biotechnol. 32(4), 341-346
9 Maier, R.M., Pepper, I.L., and Gerba, C.P. (2000) Environmental Microbiology. Academic Press. London
10 Duttka, J. and Kwan, K. (1981) Comparison of three microbial toxicity screening tests with the microtox test. Bull. Environ. Contam. Toxicol. 27, 753-757
11 Gallert, G., Stommel, A., and Trujlllano, A. (1999) Development of an optimal bacterial medium based on the growth inhibition assay with Vibrio fischeri. Chemosphere 39, 467-476   DOI   ScienceOn
12 Grady, C.P., Daigger, G.T., and Lim, H.C. (1999) Biological wastewater treatment, 2nd Ed. Marcel Dekker. New York
13 Mino, T., Van Loosdrecht, MCM, and Heijnen, J.J. (1998) Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Res. 32(11), 3193-3207   DOI   ScienceOn
14 Zafiri, C, Kornaros, M., and Lyberatos, G. (1999) Kinetic modeling of biological phosphorus removal with a pure culture of Acinetobacter sp. under aerobic, anaerobic, and transient operating conditions. Water Res. 33(12), 2769-2788   DOI   ScienceOn
15 Wentzel, M.C., Lotter, L.H., Loewenthal, R.E., and Marais, G. vR. (1986) Metabolic behaviour of Acinetobacter sp. in enhanced biological phosphorus removal: a biochemical model. Water SA 12, 209-224
16 Cho, K.S., Ryu, H.W., Lee, I.S., and Choi, H.M. (2002) Effect of solids concentration on bacterial leaching of heavy metals from sewage sludge. J. Air and Waste Manage. Assoc. 52, 237-243   DOI   ScienceOn
17 Ryu, H.W., Moon, H.S., Lee, E.Y., Cho, K.S., and Choi, H.M. (2003) Leaching characteristics of heavy metals from sewage sludge by Acidithiobacillus ihiooxidans MET. J. Environ. Qual. 32, 751-759   DOI   ScienceOn
18 Johnson, F.H., Eyring, H., and Stover, B.J. (1974) The theory of rate processes in biology and medicine. Wiley. New York
19 Bitton, G. and Koopman, B. (1992) Bacterial and enzymatic bioassays for toxicity testing in the environment. Rev. Environ. Coniam. Toxicol. 125, 1-22
20 Gellert, G. (2000) Sensitivity and significance of luminescent bacteria in chronic toxicity testing based on growth and bioluminescence. Ecotoxicol. Environ. Saf. 45, 87-91   DOI   ScienceOn
21 Torslov, J. (1993) Comparison of bacterial toxicity tests based on growth, dehydrogenase activity and esterase activity of Pseudomonas fluorescens. Ecotoxicol. Environ. Saf. 25, 33-40   DOI   ScienceOn
22 Landis, W.G. and Yu, M.H. (1995) Introduction to environmental toxicology. Lewis Publishers. London