Browse > Article
http://dx.doi.org/10.5338/KJEA.2004.23.3.148

Analysis of Multiple Pesticide Residues in Apples and Pears Using Gas-Liquid Chromatography  

Park, Ju-Hwang (Department of Agricultural Chemistry, Kyungpook National University)
Kim, Taek-Kyum (Department of Agricultural Chemistry, Kyungpook National University)
Oh, Chang-Hwan (Lab Frontier Inc.)
Kim, Jeong-Han (School of Agricultural Biotechnology, Seoul National University)
Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University)
Kim, Jang-Eok (Department of Agricultural Chemistry, Kyungpook National University)
Publication Information
Korean Journal of Environmental Agriculture / v.23, no.3, 2004 , pp. 148-157 More about this Journal
Abstract
A rapid analytical method was developed to determine multiple pesticide residues in apples and pears using gas-liquid chromatography (GLC). The samples were extracted with water-miscible solvents and purified by cleanup procedures serially comprising liquid-liquid partition and solid-phase extraction (SPE). Each analyte was separated and determined by a high-resolution GLC equipped with electron-capture detector (ECD) and nitrogen-phosphorous detector (NPD). A total of 196 pesticides, which were previously classified into 5 groups each for ECD and NPD based on their retention behaviors on the capillary column and responses to the detector, were subjected to the recovery experiment. In compliance with the analytical criteria, 70 to 120% of recovery and less than 20% relative standard deviation the proposed method could be successfully applied to analyze 136 and 133 pesticide residues in apples and pears, respectively, which enabled not only rapid screening but quantitation of the residues. Even though less reliability was resulted from unacceptable recovery range, rest of pesticides including 43 and 45 analyzes in apples and pears, could be also detected for their identity. The proposed method fliled to cover 17 and 18 pesticides for apples and pears, which mostly showed high polarity or heat-lability but could be suitable far fast surveilance or monitoring of fruit harvests.
Keywords
multiple pesticide residues; solid-phase extraction (SPE); apple; pear; GLC analysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ho, W. H. and Hsieh, S. J. (2001) Solid phase microextraction associated with microwave assited extraction of organochlorine pesticides in medicinal plants, Anatytica Chimica Acta 428, 111-120   DOI   ScienceOn
2 Ahmed, F. E. (2001) Analyses of pesticides and their metabolites in foods and drinks, Trends in anal. chem.20(11), 649-661   DOI   ScienceOn
3 Valverde, A., Aguilera, A., Rodriguez, M. and Boulaid, M. (2001) What are we determining using gas chromatographic multiresidue methods: tralomethrin or deltamethrin?, J. Chromatogr. A 943, 101-111   DOI   ScienceOn
4 Lacassie, E., Marquet, P, Gaulier, J. M., Dreyfuss, M. F.and Lachahre, G. (2001) Sensitive and specific multiresidue methods for the determination of pesticides ofvarious classes in clinical and forensic toxicology, Forensic Sci. Int'l 121, 116-125   DOI   ScienceOn
5 Lehotay, S. J. (1997) SupercnticaI fluid extraction ot pesticide in foods, J. Chromatogr. A 785, 289-312   DOI   ScienceOn
6 Sabic, H., Jeannot, R., Roudeau, B. (2000) Muldresidue methods using solid-phase extraction techniques for monitoring priority pesticides, including triazines and degradation products, in ground and surface waters, J. Chromatogr. A 885, 217-236   DOI   ScienceOn
7 김택제, 어연우(1990) 다성분 잔류농약 동시 분석법에 관한 연구(3차), KIAT 연구보고서
8 Kim, W. S., Lee, B. H. and Park, H, J. (1996) Study on the Development of Simultaneous Analytical Method for the Residual Organic Chloride Pesticides by Gas Chromatography, J. Korean Environ Sci. 5(5), 561-567
9 Niessner, G, Buchberger, W. and Eckerstorfer, R. (1999)Multiresidue screening methods for fhe determination ofpesticides in plant materials, J. Chromatogr. A 846, 341-348   DOI   ScienceOn
10 Serrano, R, Lopez, F. J. and Hemandez, F. (1999) Multiresidue determination of persistent organochlorine and organophosphorus compounds in whale tissues using automated liquid chromatographic clean up and gas chromatographic-mass spectrometric detection, J. Chromatogr. A 855, 633-643   DOI   ScienceOn
11 Colume, A., Cardenas, S., Gallego, M. and Valcairel, M.(2001) Semiautomatic multiresidue gas chromatographic method for the screening of vegetables for 25 organochlorine and pyrethroid pesticides, Anal. Chem. Acta 436,153-162   DOI   ScienceOn
12 식품의약품안전청 (1999) 식품공전 P.37-106
13 Castro,J., Sanchez-Brunete, C. and Tadeo, J. L. (2001) Multiresidue analysis of insecticides in soil by gas chromatography with electron-capture detection and confirmation by gas chromatography-mass spectrometry, J.Chromatogr. A 918, 371-380   DOI   ScienceOn
14 Zoonen, P. V. and Rijksinstituut, V. (1996) Analytical methods for pesticide residues in foodstuffs, 6th ed., Ministryof Public Health, Welfare and Sport, Netherlands
15 Kim, T. J., Eo, Y. W. and Kim, Y. S. (1987) A Study on the Separation and Determination of Organochlorine Pesticides by Capillary Column Gas-liquid Chromatography, J. Korean Chem. Soc. 31(5), 425-433
16 식품의약품안전청 (1999) 식품공전 별책 제 7. 일반시험법,P.110-256
17 Pang, G. F., Cao, Y. J. and Fan, C. L. (1995) Modification of AOAC multiresidue method for determining synthetic pyrethroid residues in fruits, vegetables and grains, Part I : Acetonitrile extraction system and optimization of Florisil cleanup and chromatography, J.AOAC International 78(6), 1481-1488
18 殘留農藥基準便覽 (1994) 日本食品衛生協會 173pp
19 C, Patricia (1995) Official metthods of analysis AOAC international, Pesticide and industrial chemical residues, 16th ed., Chapter 10, USA
20 Mcmahon, B. M. (1994) Pesticide Analytical Manual Volume I : Multiresidue Methods, 3rd ed., FDA. USA