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http://dx.doi.org/10.5338/KJEA.2004.23.1.068

Culture Optimization for Bacillus lentimorbus G-74 by Using a Miscanthus purpurascens Juice Medium  

Kang, Sun-Chul (Division of Food, Biological and Chemical Engineering, Daegu University)
Seo, Hae-Jeong (Division of Food, Biological and Chemical Engineering, Daegu University)
Publication Information
Korean Journal of Environmental Agriculture / v.23, no.1, 2004 , pp. 68-74 More about this Journal
Abstract
Miscanthus purpurascens Juice containing potassium (37,952 mg/L), nitrogen (14,000 mg/L), phosphorus (6,800 mg/L), magnesium (5,969 mg/L), calcium (5,910 mg/L), etc., was investigated to develop a novel meidum far the mass cultivation of useful microorganisms. For this research, we first isolated an antagonistic bacterium G-74 from soil, which showed strong growth inhibition against two phytopathogenic fungi, Rhizoctonia solani and Botrytis cinerea, and identified as Bacillus lentimorbus G-74 based on the morphological characteristics and MIDI analysis. Culture conditions for G-74 strain in the M. purpurascens juice medium were optimized. Dilution rate of the medium, temperature and initial pH for the optimum growth of G-74 strain were 30% (V/V), $35^{\circ}C$ and 5.0, respectively. It was found that additions of 2.0% (W/V) corn starch as a carbon source and 1.0% (W/V) yeast extract as a nitrogen source in this medium increased B. lentimorbus G-74 growth to 66% more efficient than Luria Bertani medium.
Keywords
Miscanthus purpurascens juice medium; antagonistic bacterium; Bacillus lentimorbus G-74; MIDI analysis;
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