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http://dx.doi.org/10.5338/KJEA.2002.21.1.074

Roles of Azospirillum spp. Inoculation in Two Consecutive Growth of Maize Plants  

Choi, Seung-Ju (Department of Agricultural Chemistry, Chungbuk National University)
Gadagi, Ravi (Department of Agricultural Chemistry, Chungbuk National University)
Park, Myung-Su (Department of Agricultural Chemistry, Chungbuk National University)
Yang, Jin-Chul (Department of Agricultural Chemistry, Chungbuk National University)
Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Environmental Agriculture / v.21, no.1, 2002 , pp. 74-79 More about this Journal
Abstract
Two consecutive green house experiments were carried out to examine the effect of Azospirillum spp. inoculation on growth, nitrogen and phosphorus accumulation in maize plants grown in pots. There were eight treatments including an uninoculated control and Azospirillum strains OAD-3, OAD-9, AZ-22, AZ-8, AZ-9, Azospirillum brasilense BR-11001 and Azospirillum lipoferum BR-11080. The inoculated plants showed higher values in each of the following measurements; plant height, total dry mass and nitrogen and phosphorus accumulation in shoot when compared to the uninoculated control plants in two consecutive experiments conducted in the same soil. Among Azospirillum strains, Azospirillum sp. OAD-3 inoculated plants showed higher nitrogen accumulation by 44.5% and 45.1%, total dry mass by 48.6% and 66.9% in two consecutive experiments respectively. The nitrogen concentration in the maize plants was not changed significantly in the first experiment, however it increased significantly in the second experiment due to Azospirillum inoculation. In addition, Azospirillum sp. OAD-9 and A. brasilense BR-11001 also proved to be effective with respect to total dry mass, total nitrogen accumulation and total phosphorus accumulation. The nitrogen concentrations in maize plants were increased in the second experiment due to Azospirillum inoculation.
Keywords
Azospirillum; maize; nitrogen; phosphorus; consecutive growth;
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1 Tien, T. M., Gaskin, M. H. and Hubbell, D. H. (1979) Plantgrowth substances produced by Azospirillum brasilense andtheir effect on the growth of pearl millet (Penniestum ame-ricanum), AppI. Environ. Microbiol. 37, 1016-1024
2 Balsubranium, A and Kumar, K. (1989) Influence ofAzospirillum biofertilizer on rice crop. International work-shop and symposium on biological nitrogen fixation asso-ciated with rice production, CRRI, Cuttack, p.56
3 Tippannavar, C. M. and Alagawadi, A. R(1998) Influence of Azospirillum inoculation in combination with reduced levels of nitrogen on rabi sorghum, In 39th annual confer- ence,association of microbiologist in India, Manglore, p.256
4 Murthy M. G. and Ladha, J. K. (1988) InHuence of Azos-pirillum inoculation on the mineral uptake and growth ofrice under hydroponic conditions, Plant SoiI 108, 115-118
5 James, E. K. (2000) Nitrogen fixation in endophytic andassociative symbiosis, Field Crops Res. 65, 197-209   DOI   ScienceOn
6 Okon, Y. and Itigsohn, R. (1995) The development ofAzospirillum as a commercial inoculants for improving cropyields, Biotechnol Adv. 13, 415-424   DOI   ScienceOn
7 James, E. K. and Olivares, F. L. (1998) Infection andcolonization of sugarcane and other graminaceous plants byendophytic diazotrophs, Crit. Rev. Plant Sci. 17, 77-119   DOI
8 Nelson, D. W. and Sommer, L. E. (1973) Determination of total nitrogen in plant material, Agron. J. 65, 109-112   DOI
9 Murphy, J. and Riley, J. P. (1962) A modified single solu-tion method for determination of phosphate in naturalwater, Anal. Chem. Acta. 27, 31-36   DOI   ScienceOn
10 Dobereiner, J. and Day J. M. (1976) Associative symbiosisand free living systems, In Newton, W. E. and Nyman, C.J. (Eds.) Proceedings of the first international symposiumon nitrogen fixation, Washington State University Press,Pullman, p.518-538
11 Okon, Y. and Labandera-Gonzalez, C. A. (1994) Agronomicapplications of Azospirillum, An evaluation of 20 years wor-Idwide field inoculation, Soil Biol. Biochem. 26, 1591-1601
12 Rangarajan, M., Kandsamy, D., Naryanan, R, Ponniah, C.,Govindarjan, K. and Oblisami, G. (1987) Exploitation ofmicrobial system associated with plant species from theeco-development of western ghats region, Final technical report of D. 0. E. Project, p.38
13 Subba Rao, N. S. (1993) Interaction of nodulated treespecies with other microorganisms and plants. In SubbaRao, N. S. and Rodriguez-Barruecco, C. (Eds.) Symbiosis innitrogen fixing trees, Oxford and IBH publishing Co. Pvt.Ltd. New Delhi, p.54-84
14 Gadagi, Ravi. (1999) Studies on Azospirillum isolates ofornamental plants and their effect on Gaillardia pulchella.Ph.D. thesis, University of Agricultural Sciences, Dharwad,India
15 Black, C. A. (1968) Soil plant relations-nitrogen fixations inplants, John Willey and Sons publications, p.626
16 Srestha, R. K. and Ladha, J. K. (1996) Genotypic variationin promotion of rice nitrogen fixation as determined bynitrogen $^1^5N$ dilution, Soil Sci. Soc. Am. J. 60, 1815-1821   DOI   ScienceOn
17 Fallik, E. and Okon, Y. (1996) Inoculants of Azospirillumbrasilense: Biomass production, survival and growth promo-tion of Setaria italica and Zea mays, SoiI Biol. Biochem. 28,123-126   DOI   ScienceOn
18 Kennedy, I. R, Pereg-gerk, L. L, Wood, C, Deaker, R.,Gilchrist, K. and Katupitiya. S. (1997) Biological nitrogenfixations in non-leguminous field crops facilitating theevaluation of an effective association between Azospirillumand wheat, Plant Soil. 194, 65-79   DOI   ScienceOn
19 Boddey, R M. (1995) Biological nitrogen fixation in sugarcane : a key to energetically viable biofuel production, Crit.Rev. Plant Sci. 14, 263-279   DOI   ScienceOn
20 Boddey, R. M., De Oliveira, O. C., Urquiaga, S., Reis, V. M., Olivarares, F. L., Baldani, V. L. D. and Dobereiner, J.(1995) Biological nitrogen fixation associated with sugarcane and rice contributions and prospectors for improvement, Plant Soil. 174, 195-209   DOI
21 Dobereiner, J., Baldani, V. L. D. and Reis, V. M. (1995)Endophytic occurrence of diazotrophic bacteria in non-leguminous crops, In Fendiik, I., Del Gallo, M., Van-deleyden, J., de Zamaroczy, M. (Eds.) Azospirillum VI andrelated microorganisms, Springer, Berlin, p.3-14
22 Elmench, C. (1984) Molecular biology and ecology of diazo-trophs associated with non-legumes plants, Biotechnol. 2,967-978   DOI
23 kon, Y. (1985) Azospirillum as a potential inoculants foragriculture, Trends. Biotechnol. 3, 223-228
24 Michiels, K., Vanderleyden, J. and Van Gool, A. (1989) Azo-spirillum-plant associations as affected by nitrate andammonium supply, Isotopes Environ. Health study. 34, 67-73
25 Tal, S. and Okon, Y. (1985) Production of the reservematerial poly-$\beta-hydroxybutyrate and its function in Azosp-irillum brasilense Cd, Can. J. Bicrobiol. 31, 608-613   DOI
26 Hartmann, A. and Zimmer, W. (1994) Physiology of Azospirillum. In Okon, Y. (Ed.) Azospirillum/plant associations, CRC Press Boca, Raton, FL. p. 15-39
27 Lamm, R B. and Neyra, C. A. (1981) Characterization and cyst production of azospirilla isolated from selected grass growing in New Jersey and New York, Can. J. Microbiol. 27, 1320-1325   DOI
28 Sadasivan, L. and Neyra, C. A. (1987) Cyst production and brown pigment formation in aging cultures of Azospirillum brasilense ATCC 29145, J. Bacterial. 169, 1670-1677   DOI
29 Tal, S., Smimoff, P. and Okon, Y. (1990) The regulation ofpoly-$\beta-hydroxybutyrate metabolism in Azospirillum brasilenseduring balanced growth and starvation, J. Gen. Microbiol.136, 1191-1196   DOI