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http://dx.doi.org/10.15433/ksmb.2019.11.2.081

Effects of Nitrogen and Phosphorus Starvation on Growth and Fatty Acid Production in Newly Isolated Two Freshwater Green Microalgae from Nakdonggang River  

Yim, Kyung June (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Park, Hanwool (Department of Marine Science & Biological Engineering, Inha University)
Lee, Chang Soo (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Jo, Bok Yeon (Bioresources Industrialization Support Department, Nakdonggang National Institute of Biological Resources)
Nam, Seung Won (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Lee, Choul-Gyun (Department of Marine Science & Biological Engineering, Inha University)
Kim, Z-Hun (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.11, no.2, 2019 , pp. 81-88 More about this Journal
Abstract
In this study, effects of nitrogen (N) and phosphorus (P) starvation on the cell growth and fatty acid (FA) production of newly isolated freshwater microalgae were investigated. The microalgae were identified as Chlorella sp. and Parachlorella sp. through 18S rRNA sequencing. Optimal culture temperature and light intensity were investigated using a high-throughput photobioreator, and the result was validated in 0.5 L bubble column photobioreactors using BG-11 without NaNO3 and/or K2HPO4. Under nutrient starvation conditions, total FA contents of the microalgae were significantly changed rather than FA composition. Starvation of both N and P was most effective for increasing FA contents in Parachlorella sp (24.4±0.1%) whereas highest FA contents (42.6±1.8%) was achieved when only P was starved in Chlorella sp. among tested conditions. These results suggest an effective strategy for increasing FA production from microalgae using appropriate nutrient starvation.
Keywords
microalgae; nutrient stress; Chlorella; Parachlorella; fatty acid;
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