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http://dx.doi.org/10.15433/ksmb.2019.11.2.042

Antibacterial and Antioxidant Activity of Spermidine, a Natural Polyamine, on Fish Pathogenic Bacteria and C2C12 Myoblast Cells  

Hwang-Bo, Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
Choi, Eun-Ok (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
Kim, Min Young (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
Ji, Seon Yeong (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
Hong, Su Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
Park, Cheol (Department of Molecular Biology, College of Natural Sciences, Dong-eui University)
Cha, Hee-Jae (Department of Parasitology and Genetics, Kosin University College of Medicine)
Kim, Suhkmann (Department of Chemistry, Pusan National University)
Kim, Heui-Soo (Department of Biological Sciences, Pusan National University)
Hwang, Hye-Jin (Department of Food and Nutrition, College of Nursing, Healthcare Sciences & Human Ecology, Dong-eui University)
Choi, Yung Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.11, no.2, 2019 , pp. 42-51 More about this Journal
Abstract
We compared the antibacterial activities of spermidine and astaxanthin against two gram-positive bacteria such as Streptococcus parauberis and S. iniae to find new antibacterial candidates. We also evaluated the preventive effects of spermidine against oxidative stress-induced cytotoxicity in C2C12 myoblasts. Our results indicated that spermidine has more significant antibacterial activities than astaxanthin against both two fish pathogenic bacteria as well as gram-negative bacteria Escherichia coli used as a control group. Minimum inhibitory concentration and minimum bactericidal concentration of spermidine were 0.25 mM and 1 mM against S. parauberis, 1 mM and 3 mM against S. iniae, and 0.5 mM and 1.5 mM against E. coli, respectively. In addition, the postantibiotic effect lasted from 7 h, 5 h and 6 h for S. parauberis, S. iniae and E. coli, respectively. The results also showed that the decreased C2C12 cell viability by H2O2 could be attributed to the induction of DNA damage and apoptosis accompanied by the increased production of reactive oxygen species, which was remarkably protected by spermidine. Additionally, the antioxidant effect of spermidine was associated with the activation of Nrf2 signaling pathway. According to the data, spermidine may be a potential lead compound which can be further optimized to discover novel antibacterial and antioxidant agents.
Keywords
Spermidine; Antibacterial and antioxidant activity; ROS; DNA damage; Apoptosis;
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