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http://dx.doi.org/10.15433/ksmb.2018.10.1.044

Whole-cell Biotransformation of Chlorella Oil Hydrolysates into Medium Chain Fatty Acids  

Seo, Joo-Hyun (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
Min, Won-Ki (Department of Food Science and Industry, Kyungil University)
Lee, Jung-Hoo (Department of Food Science and Engineering, Ewha Womans University)
Lee, Sun-Mee (Department of Food and Nutrition, Daejeon University)
Lee, Choul-Gyun (National Marine Bioenergy Research Consortium & Department of Biological Engineering, Inha University)
Park, Jin-Byung (Department of Food Science and Engineering, Ewha Womans University)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.10, no.2, 2018 , pp. 44-52 More about this Journal
Abstract
A synthetic pathway, which consisted of fatty acid double bond hydratase, alcohol dehydrogenase, and Baeyer-Villiger monooxygenase, was applied to Chlorella oil to produce ester fatty acids, which can be hydrolyzed into medium chain fatty acids. Since linoleic acid is a major fatty acid constituent of Chlorella oil, a fatty acid double bond hydratase from Lactobacillus acidophilus NBRC13951, which is able to convert linoleic acid into 13-hydroxyoctadec-9-enoic acid, was used. Recombinant Escherichia coli expressing the fatty acid double bond hydratase from L. acidophilus NBRC13951 successfully transformed linoleic acid in Chlorella oil hydrolysates into 13-hydroxyoctadec-9-enoic acid with approximately 60% conversion yield. 13-Hydroxyoctadec-9-enoic acid was further converted into ester fatty acids by the recombinant E. coli expressing a long chain secondary alcohol dehydrogenase and a Baeyer-Villiger monooxygenase. The resulting ester fatty acids were then hydrolyzed into medium chain fatty acids by a lipase. Overall, industrially relevant medium chain fatty acids were produced from Chlorella oil hydrolysates. Thereby, this study may contribute to biosynthesis of medium chain fatty acids from microalgae oils as well as long chain fatty acids.
Keywords
Chlorella oil; medium chain fatty acids; linoleic acid; fatty acid double bond hydratase; Baeyer-Villiger monooxygenase;
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