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http://dx.doi.org/10.15433/ksmb.2018.10.1.001

Characterization of β-Secretase Inhibitory Peptide Purified from Blackfin flounder (Glyptocephalus stelleri) Protein Hydrolysate  

Lee, Jung Kwon (Department of Marine Biotechnology, Gangneung-Wonju National University)
Kim, Sung Rae (Department of Marine Biotechnology, Gangneung-Wonju National University)
Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.10, no.1, 2018 , pp. 1-8 More about this Journal
Abstract
The objective of this study was to purify and characterize the ${\beta}-secretase$ inhibitor from enzymatic hydrolysates of blackfin flounder muscle, for development of a novel anti-dementia agent that may be used in the drug or functional food industries. ${\beta}-secretase$ inhibitory peptide was purified from various enzymatic hydrolysates of blackfin flounder muscle. Among six enzymatic hydrolysates, the Alcalase hydrolysate revealed highest ${\beta}-secretase$ inhibitory activity. Consecutive purification of the blackfin flounder muscle hydrolysate using Sephadex G-25 column chromatography and octadecylsilane C18 reversed phase HPLC techniques were used to isolate a potent ${\beta}-secretase$ inhibitory peptide composed of 5 amino acids, Leu-Thr-Gln-Asp-Trp (MW: 526.7 Da). The $IC_{50}$ value of purified ${\beta}-secretase$ inhibitory peptide was $126.93{\mu}M$. Results of this study suggest that peptides derived from blackfin flounder muscle may be beneficial as anti-dementia compounds in functional foods or as pharmaceuticals.
Keywords
Alzheimer's disease; ${\beta}-secretase$ inhibitory activity; Blackfin flounder muscle; Alcalse; Anti-dementia;
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