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http://dx.doi.org/10.17135/jdhs.2016.16.1.70

A Biocompatibility Evaluation of Hydroxyapaite·Titania Surface for Dental Implant  

Kang, Min-Kyung (Department of Dental Hygiene, Hanseo University)
Bae, Sung-Suk (Department of Dental Hygiene, Hanseo University)
Publication Information
Journal of dental hygiene science / v.16, no.1, 2016 , pp. 70-76 More about this Journal
Abstract
The objective of this study was to fabricate hydroxyapatite (HA) containing titania layer by HA blasting and anodization method to obtain advantages of both methods and evaluated biocompatibility. To fabricate the HA containing titania layer on titanium, HA blasting treatment was performed followed by microarc oxidation (MAO) using the electrolyte solution of 0.04 M ${\beta}$-glycerol phosphate disodium salt n-hydrate and 0.4 M calcium acetate n-hydrate on the condition of various applied voltages (100, 150, 200, 250 V) for 3 minutes. The experimental group was divided according to the surface treatment procedure: SM (simple machined polishing treatment), HA, MAO, HA+MAO 100, HA+MAO 150, HA+MAO 200, HA+MAO 250. The wettability of surface was observed by contact angle measurement. Biocompatibility was evaluated by cell adhesion, and cell differentiation including alkaline phosphatase activity and calcium concentration with MC3T3-E1 cells. The porous titanium oxide containing HA was formed at 150 and 200 V. These surfaces had a more hydrophilic characteristic. Biocompatibility was demonstrated that HA titania composite layer on titanium showed enhanced cell adhesion, and cell differentiation. Therefore, these results suggested that HA containing titania layer on titanium was improved biological properties that could be applied as material for dental implant system.
Keywords
Anodization; Blasting; Dental implants; Hydroxyapatite; Titania;
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1 Han JH, Kim KE: Comparion of expectation and satisfaction of implant patients in pre-post implant therapy. J Dent Hyg Sci 11: 121-127, 2011.
2 Liu X, Chu PK, Ding C: Surface modification of titanium, titanium alloys, and related materials for biomedical applications. Mater Sci Eng R-Rep 47: 49-121, 2004.   DOI
3 Coelho PG, Granjeiro JM, Romanos GE, et al.: Basic research methods and current trends of dental implant surfaces. J Biomed Mater Res B Appl Biomater 88: 579-596, 2009.
4 Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y: Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 23: 844-854, 2007.   DOI
5 Ishikawa K, Miyamoto Y, Nagayama M, Asaoka K: Blast coating method: new method of coating titanium surface with hydroxyapatite at room temperature. J Biomed Mater Res 38: 129-134, 1997.   DOI
6 Kang MK, Moon SK, Kim KN: An evaluation of antibacterial titanium surface for dental implant. J Dent Hyg Sci 11: 405-410, 2011.
7 O'Hare P, Meenan BJ, Burke GA, Byrne G, Dowling D, Hunt JA: Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique. Biomaterials 31: 515-522, 2010.   DOI
8 Ishizawa H, Ogino M: Formation and characterization of anodic titanium oxide films containing Ca and P. J Biomed Mater Res 29: 65-72, 1995.   DOI
9 Yang B, Uchida M, Kim HM, Zhang X, Kokubo T: Preparation of bioactive titanium metal via anodic oxidation treatment. Biomaterials 25: 1003-1010, 2004.   DOI
10 Ishizawa H, Ogino M: Characterization of thin hydroxyapatite layers formed on anodic titanium oxide films containing Ca and P by hydrothermal treatment. J Biomed Mater Res 29: 1071-1079, 1995.   DOI
11 Kang MK, Moon SK, Kwon JS, Kim KM, Kim KN: Characterization of hydroxyapatite containing a titania layer formed by anodization coupled with blasting. Acta Odontol Scand 72: 989-998, 2014.   DOI
12 Elias CN, Oshida Y, Lima JHC, Muller CA: Relationship between surface properties (roughness, wettability and morphology) of titanium and dental implant removal torque. J Mech Behav Biomed Mater 1: 234-242, 2008.   DOI
13 Macak JM, Tsuchiya H, Ghicov A, et al.: TiO2 nanotubes: self-organized electrochemical formation, properties and applications. Curr Opin Solid State Mat Sci 11: 3-18, 2007.   DOI
14 Bico J, Thiele U, Quere D: Wetting of textured surfaces. Colloids Surf A 206: 41-46, 2002.   DOI
15 Zhu X, Chen J, Scheideler L, Reichl R, Geis-Gerstorfer J: Effects of topography and composition of titanium surface oxides on osteoblast responses. Biomaterials 25: 4087-4103, 2004.   DOI
16 Park JW, Jang JH, Lee CS, Hanawa T: Osteoconductivity of hydrophilic microstructured titanium implants with phosphate ion chemistry. Acta Biomater 5: 2311-2321, 2009.   DOI
17 Anselme K: Osteoblast adhesion on biomaterials. Biomaterials 21: 667-681, 2000.   DOI
18 Beck GR: Inorganic phosphate as a signaling molecule in osteoblast differentiation. J Cell Biochem 90: 234-243, 2003.   DOI
19 Kieswetter K, Schwartz Z, Hummert TW, et al.: Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. J Biomed Mater Res 32: 55-63, 1996.   DOI
20 Boyan BD, Batzer R, Kieswetter K, et al.: Titanium surface roughness alters responsiveness of MG63 osteoblast-like cells to $1{\alpha},25-(OH)_2D_3$. J Biomed Mater Res 39: 77-85, 1998.   DOI
21 Lee SI: The role of NFATC1 on osteoblastic differentiation in human periodontal ligament cells. J Dent Hyg Sci 15: 488-494, 2015.   DOI
22 Giordano C, Sandrini E, Busini V, et al.: A new chemical etching process to improve endosseous implant osseointegration: In vitro evaluation on human osteoblast-like cells. Int J Artif Organs 29: 772-780, 2006.   DOI
23 Anderson H: Matrix vesicles and calcification. Curr Rheumatol Rep 5: 222-226, 2003.   DOI
24 Le Guehennec L, Lopez-Heredia MA, Enkel B, Weiss P, Amouriq Y, Layrolle P: Osteoblastic cell behaviour on different titanium implant surfaces. Acta Biomater 4: 535-543, 2008.   DOI
25 Lian JB, Stein GS: Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med 3: 269-305, 1992.   DOI
26 Beck GR: Inorganic phosphate as a signaling molecule in osteoblast differentiation. J Cell Biochem 90: 234-243, 2003.   DOI
27 Chehroudi B, McDonnell D, Brunette DM: The effects of micromachined surfaces on formation of bonelike tissue on subcutaneous implants as assessed by radiography and computer image processing. J Biomed Mater Res 34: 279-290, 1997.   DOI