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http://dx.doi.org/10.17135/jdhs.2015.15.2.98

Effect of Mutan Isolated from Streptococcus mutans on the Healing of Bone Defect in Rat  

Kwon, Hyun-Jung (Department of Dental Hygiene, Gimcheon University)
Kim, Yong Hyun (Department of Life Science and Biotechnology, Soonchunhyang University)
Han, Kook-Il (Department of Life Science and Biotechnology, Soonchunhyang University)
Jung, Eui-Gil (Department of Life Science and Biotechnology, Soonchunhyang University)
Han, Man-Deuk (Department of Life Science and Biotechnology, Soonchunhyang University)
Publication Information
Journal of dental hygiene science / v.15, no.2, 2015 , pp. 98-105 More about this Journal
Abstract
We investigated the effects of a mutan (water-insoluble ${\alpha}$-glucans) isolated from Streptococcus mutans on the healing of bone defect in rat. Sprague-Dawley rats were divided into control (saline-treated), lipopolysaccharide (LPS)-treated, and mutan-treated groups (n=6 per group). Experimental bone defects were surgically created with round fissure bur at the buccal surface of the left mandibular. The control groups was administered with saline solution (0.1 ml/100 g), while the LPS and mutan group was given LPS and mutan (1 mg/kg body weight) three times weekly. After 4 weeks the rats were sacrificed, the healing of bone defect was assessed by bone mineral density (BMD) and micro-computed tomography (${\mu}CT$) examination. Percent bone volume (bone volume/tissue volume [BV/TV]), trabecular thickness (Tb.Th), and trabecular number (Tb.N) parameters of ${\mu}CT$ showed higher values in control group than LPS and mutan group. Bone surface/volume ratio (BS/BV), trabecular bone pattern factor (Tb.Pf), and structure model index parameters of ${\mu}CT$ showed higher values in LPS group than mutan group. BMD values of mutan treated-alveolar bones were significantly lower for than that of the LPS group. Therefore, we suggest that mutan, water-insoluble ${\alpha}$-glucans from S. mutans may be induce the induction of periodontal diseases.
Keywords
Bone defect; Micro-computed tomography; Mutan; Streptococcus mutans; ${\alpha}$-1,3-glucan;
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