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http://dx.doi.org/10.17135/jdhs.2015.15.2.209

The Interactive Effect of These Bacterial Substrates on the Growth of Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis  

Kim, A-Reum (Department of Dental Hygiene, College of Health Science, Eulji University)
Jeong, Moon-Jin (Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University)
Ahn, Yong-Soon (Department of Dental Hygiene, College of Health Science, Eulji University)
Kim, Mi-Na (Department of Dental Hygiene, College of Health Science, Eulji University)
Kim, Sung-Im (Department of Dental Hygiene, College of Health Science, Eulji University)
Lim, Do-Seon (Department of Dental Hygiene, College of Health Science, Eulji University)
Publication Information
Journal of dental hygiene science / v.15, no.2, 2015 , pp. 209-219 More about this Journal
Abstract
In order to explore an effect of interaction of Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis that are bacteria relevant to periodontal disease on its growth, the bacteria were incubated in trypticase soy hemin menadione broth at $37^{\circ}C$ $CO_2$ incubator for 7 days through anaerobic jar by single and co-culture with heat treated dead bacteria under anaerobic gas pack. In order to confirm growth level, absorbance was measured and for confirming colony structure and form, it was observed with scanning electron microscope. In order to confirm an effect on pathogenicity of P. gingivalis, real time reverse transcriptase polymerase chain reaction was implemented for expression analysis for rgpA gene that produces HRgpA which is gingipain. As a result, the following conclusion was obtained. Colony formation of S. gordonii and P. gingivalis was increased by other dead bacteria and in case of F. nucleatum, its colony formation was showed an aspect of being increased by dead bacterium of P. gingivalis but decreased by dead bacterium of S. gordonii. Therefore, it is considered that the strains being used for this study would affect interactively through bacterial cell itself as well as their interaction factor at the time of colony formation.
Keywords
Fusobacterium nucleatum; Microbial interactions; Porphyromonas gingivalis; Streptococcus gordonii;
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