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The Effect of Polycan-Calcium Gluconate Complex on Inflammatory Mediators from Periodontitis Patients  

Kim, Ji-Hye (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Kim, Ki-Rim (Department of Dental Hygiene, Kyungpook National University)
Jin, Hye-Jung (Department of Dental Hygiene, Dong-eui University)
Im, Sang-Uk (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Song, Keun-Bae (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Choi, Youn-Hee (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Publication Information
Journal of dental hygiene science / v.14, no.2, 2014 , pp. 223-229 More about this Journal
Abstract
The aim of this study was to examine the effect of polycan-calcium gluconate complex on the levels of immune and inflammatory mediators in gingival crevicular fluid and serum from patients with periodontitis. A total of 39 patients with periodontitis took placebo (placebo group) or polycan-calcium gluconate complex (treatment group) twice a day for 4 weeks. At baseline and 4 weeks, gingival crevicular fluid (GCF) and blood was collected from each subjects. The secretion level of interleukin $(IL)-1{\beta}$ in GCF was measured by enzyme-linked immunosorbent assay (ELISA). Western blot analysis was conducted to determine the expression of matrix metalloproteinase (MMP)-8 and tumor necrosis factor $(TNF)-{\alpha}$ in GCF. Serum samples were analysed for MMP-8 by ELISA and C-reactive protein (CRP) by turbidimetric immuno assay. MMP-8 and $TNF-{\alpha}$ were significantly decreased in the treatment group at 4 weeks. The level of $IL-1{\beta}$ in the treatment group was significantly lower than those of the placebo group. No differences were observed in CRP levels. Taken together, these data indicate that taking of polycan-calcium gluconate complex led to reduction of inflammatory biomarkers in serum and GCF of periodontitis patients.
Keywords
Calcium gluconate; Gingival crevicular fluid; Inflammation biomarkers; Periodontitis; Polycan;
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1 Marcaccini AM, Meschiari CA, Zuardi LR, et al.: Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy. J Clin Periodontol 37: 180-190, 2010.   DOI   ScienceOn
2 Choi YH, Kim JH, Kim JY, Lee YK, Yang HY, Song KB: The improvement of gingival health by polycan-calcium gluconate complex. J Korean Acad Oral Health 37: 175-179, 2013.   DOI
3 Hwang SJ, Kim YK, Yang SJ, Cho HJ: Influence of smoking on matrix metalloproteinase-9 in the gingival crevicular fluid. J Dentl Hyg Sci 11: 339-344, 2011.
4 Armitage GC: Analysis of gingival crevice fluid and risk of progression of periodontitis. Periodontol 2000 34: 109-119, 2004.   DOI   ScienceOn
5 Ku SK, Cho HR, Sung YS, Kang SJ, Lee YJ: Effects of calcium gluconate on experimental periodontitis and alveolar bone loss in rats. Basic Clin Pharmacol Toxicol 108: 241-250, 2011.   DOI   ScienceOn
6 Shin HD, Yang KJ, Park BR, Son CW, Jang HJ, Ku SK: Antiosteoporotic effect of polycan, beta-glucan from Aureobasidium, in ovariectomized osteoporotic mice. Nutrition 23: 853-860, 2007.   DOI   ScienceOn
7 Song HB, Park DC, Do GM, et al.: Effect of exopolymers of Aureobasidium pullulans on improving osteoporosis induced in ovariectomized mice. J Microbiol Biotechnol 16: 37-45, 2006.
8 Kim YS, Kang SJ, Kim JW, et al.: Effects of polycan, a ${\beta}$- glucan, on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats. J Periodontal Res 47: 800-810, 2012.   DOI   ScienceOn
9 Cavallini M, de Boccard F, Cirsu MM, Fassati RL, Baruffaldi Preis FW: Serum pro-inflammatory cytokines and chemical acid burns in rats. Ann Burns Fire Disasters 17: 84-87, 2004.
10 Karnad AS, Patil PA, Majagi SI: Calcium enhances antiinflammatory activity of aspirin in albino rats. Indian J Pharmacol 38: 397-402, 2006.   DOI
11 Mombelli A: Clinical parameters: biological validity and clinical utility. Periodontol 2000 39: 30-39, 2005.   DOI   ScienceOn
12 Lamster IB, Ahlo JK: Analysis of gingival crevicular fluid as applied to the diagnosis of oral and systemic disease. Ann NY Acad Sci 1098: 216-229, 2007.   DOI   ScienceOn
13 Konopka L, Pietrzak A, Brzezinska-Blaszczyk E: Effect of scaling and root planing on interleukin-$1{\beta}$, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients. J Periodontal Res 47: 681-688, 2012.   DOI   ScienceOn
14 Paraskevas S, Huizinga JD, Loos BG: A systematic review and meta-analyses on C-reactive protein in relation to periolontitis. J Clin Periodontol 35: 277-290, 2008.   DOI   ScienceOn
15 Tecucianu JF: Double-blind clinical study of a titrated extract of an unsaponifiable fraction of Zea mays (L) on gingival inflammation. Inf Dent 57: 21-32, 1975.
16 Golub LM, Lee HM, Stoner JA, et al.: Subantimicrobial-dose doxycycline modulates gingibal crevicular fluid biomarkers of periodontitis in postmenopausal osteopenic women. J Periodontol 79: 1409-1418, 2008.   DOI   ScienceOn
17 Sanders AE, Slade GD, Fitzsimmons TR, Bartold PM: Physical activity, inflammatory biomarkers in gingival crevicular fluid and periodontitis. J Clin Periodontol 36: 388-395, 2009.   DOI   ScienceOn
18 Fitzsimmons TR, Sanders AE, Bartold PM, Slade GD: Local and systeimc biomarkers in gingival crevicular fluid increase odds of periodontitis. J Clin Periodontol 37: 30-36, 2010.   DOI   ScienceOn
19 Choi SM, Han SB, Hwang KS: Clinical trial of unsaponifiable fraction of zea mays L. on healing after surgical periodontal therapy in human. J Periodontal Implant Sci 19: 63-70, 1989.
20 Chan GCF, Chan WK, Sze DMY: The effect of ${\beta}$-glucan on human immune and cancer cells. J Hematol Oncol 2: 1-11, 2009.   DOI   ScienceOn
21 Song HS, Moon KY: In vitro antioxidant activity profiles of ${\beta}$-glucan isolated from yeast Saccharomayces cerevisiae and mutant Saccharomayces cerevisiae IS2. Food Sci Biotechnol 15: 437-440, 2006.
22 Novak M, Vetvicka V: Beta-glucans, history, and the present: immunomodulatory aspects and mechanisms of action. J Immunotoxicol 5: 47-57, 2008.   DOI   ScienceOn
23 Seo HP, Kim JM, Shin HD, et al.: Production of ${\beta}$-1,3/1,6-glucan by Aureobasidium pullulans SM-2001. Korean J Bitechnol Bioeng 17: 376-380, 2002.
24 Darveau RP: Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol 8: 481-490, 2010.   DOI   ScienceOn
25 Jung JO, Lee KH, Youn HJ: The correlation between diabetes and periodontitis in Korean adults. J Dentl Hyg Sci 11: 221-228, 2011.
26 Figueredo CM, Ribeiro MS, Fischer RG, Gustafsson A: Increased interleukin-$1{\beta}$ concentration in gingival crevicular fluid as a characteristic of periodontitis. J Periodontol 70: 1457-1463, 1999.   DOI   ScienceOn
27 Offenbacher S, Barros SP, Beck JD: Rethinking periodontal inflammation. J Periodontol 79: 1577-1584, 2008.   DOI   ScienceOn
28 Sorsa T, Uitto VJ, Suomalainen K, Vauhkonen M, Lindy S: Comparison of interstitial collagenases from human gingiva, sulcular fluid and polymorphonuclear leukocytes. J Periodontal Res 23: 386-393, 1988.   DOI
29 Yucel-Lindberg T, Båge T: Inflammatory mediators in the pathogenesis of periodontitis. Expert Rev Mol Med 15: e7, 2013.   DOI   ScienceOn
30 Graves DT, Cochran D: The contribution of interleukin-1 and tumor necrosis factor to periodontal tissue destrucion. J Periodontol 74: 391-401, 2003.   DOI   ScienceOn
31 Lee SY, Jung YH, Kim KH, et al.: Matrix metalloproteinases and tissue inhibitors of matrix metallo-proteinases in gingival crevicular fluids of periodontitis patients. J Korean Acad periodontol 34: 139-148, 2004   DOI