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The Effect of Osteoblast like Cell on Low Intensity Pulsed Ultrasound Used in Current Clinical Practice  

Park, Sang-Rye (Department of Dental Hygiene, Kyungnam College of Information & Technology)
Kim, Ji-Young (Department of Dental Hygiene, Kyungnam College of Information & Technology)
Choi, Byul Bo-Ra (Department of Dental Hygiene, Kyungnam College of Information & Technology)
Publication Information
Journal of dental hygiene science / v.14, no.2, 2014 , pp. 101-106 More about this Journal
Abstract
In this study, we investigated the proliferative and adhesional effect of human osteoblast like MG-63 cell treated with low intensity pulsed ultrasound (LIPUS). We tested the effectiveness of LIPUS in human osteoblast like MG-63 cells. Cell proliferation was measured using a water soluble tetrazolium salts-1 assay. The mRNA expression of alkaline phosphate, vascular endothelial growth factor (VEGF), integrin alpha 2, colla 1A1 were performed by reverse transcription-polymerase chain reaction. LIPUS was no cytotoxicity in human osteoblast like MG-63 cells. In addition, the data show that treatment with 1 MHz and 3 MHz LIPUS on increased proliferation 7 days after. There were significant increased in mRNA expression of alkaline phosphatase, osteocalcin, VEGF, integrin alpha 2 and colla 1A1 (p<0.05). Therefore, the LIPUS significantly increased differential expression of mRNA levels in osteoblast like MG-63 cell and new possibilities in dental clinical practice.
Keywords
Low-intensity focused ultrasound; Osteoblasts; Cell proliferation; Vascular endothelial growth factor A;
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Times Cited By KSCI : 4  (Citation Analysis)
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