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http://dx.doi.org/10.7231/JON.2012.23.3.143

A Thirteen Week Repeated Oral Dose Toxicity Test and A Four Week Recovery Test of ACM(Added Chongmyung-tang) in Sprague-Dawley Rats  

Park, Dae-Myung (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
Lee, Sang-Ryong (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
Lim, Jong-Soon (Dept. of Life Science and Preventative Medicine College of Oriental Medicine, DaeJeon University)
Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, DaeJeon University)
Jung, In-Chul (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Oriental Neuropsychiatry / v.23, no.3, 2012 , pp. 143-160 More about this Journal
Abstract
Objectives : To provide information on the safety of ACM, we carried out a 13-week repeated oral dose toxicity and a 4-week recovery test of ACM in Sprague-Dawley rats. Methods : Female and male rats were treated with ACM with oral doses of 800, 2000, and 5000 mg/kg. The ACM was administered for 13 weeks. Mortality, clinical signs, body weight changes, food consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights and histological markers were monitored during the study period. Moreover, the rats were monitored for 4 extra weeks to determine recovery time after the study period. Results : We found no mortality and no abnormalities in clinical signs, body weight, food consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights and histological markers in any of the rats tested. Conclusions : The no-observed adverse effects level (NOAEL) was considered as over 5000 mg/kg for male and female rats.
Keywords
ACM; Added Chongmyung-Tang; 13 week repeated oral dose toxicity test; 4 week recovery test; SD rats;
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1 John V, Beck JP, Bienkowski MJ, Sinha S, Heinrikson RL. Human beta-secretase (BACE) and BACE inhibitors. J. Med. Chem. 2003;46(22):4625-30.   DOI   ScienceOn
2 Je JY, Kim SK. Water-soluble chitosan derivatives as a BACE1 inhibitor. Bioorg. Med. Chem. Lett. 2005;13:6551-5.   DOI   ScienceOn
3 Cheong MH, Lee SR, Yoo HS, Jeong JW, KimgY, Kim WJ, Jung IC, Choi YH. Anti-inflammatory effects of Polygala tenuifolia root through inhibition of NF-${\kappa}B$ activation in lipopolysaccharide-induced BV2 microglial cells. Journal of ethnopharmacology. 2011;137(3):1402-8.   DOI   ScienceOn
4 Park EK, Shim ES, Jung HS, Sohn NW, Sohn YJ. Effects of chongmyung-tang, polygalae radix and acorigraminei rhizoma on A$\beta$ toxicity and memory dysfunction in mice. Korean J. Orient. Int. Med. 2008;29(3):608-20.
5 Choi WC, Jung IC, Lim JS, Kim SH, Lee SR. Single oral dose toxicity test of ACM(Added Chongmyung-tang) in Sprague-Dawley rat. Journal of oriental neuropsychiatry. 2012; 23(2):121-8.   DOI   ScienceOn
6 Department of the national association of Herbalogy. Herbalogy. Seoul:Youngrimsa. 2008:411-2,594-5.
7 Kim HS, Jung IC, Lee SR. Effects of Morinda officinalis(MDOF) on inhibition of impairment of learning and memory, and acetylcholinesterase in amnesia mice. Journal of oriental neuropsychiatry. 2003; 14(1):45-58.
8 Chae JG, Jung IC, Lee SR. The effects of Crataegus prinnatiflora BGE. var. major N.E. BR(CPVM) extract on the the Alzheimer's disease model induced by CT105. Journal of oriental neuropsychiatry. 2002;13(1):79-115.
9 Ha SY, Jung IC, Lee SR. The effects of ChongMyungTang(CMT) and SanSaChongMyungTang (SCMT) on the Alzheimer's disease model. Journal of oriental neuropsychiatry. 2006; 17(1):59-78.
10 Lim JH, Jung IC, Lee SR. The effects of ChongMyungTang(CMT) and ChongMyungTang added Moutan Cortex(PCMT) hot water extract & ultra-fine powder on microglia and memory deficit model. Korean journal of oriental physiology & pathology. 2006;20(4): 997-1008.
11 Lim JH, Jung IC, Lim JS, Kim SH, Lee SR. Effect of Chongmyung-Tang prescription combination on the production of amyloid $\beta$ protein and $\beta$-site amyloid precursor protein-cleaving enzyme activity in vitro. Journal of oriental neuropsychiatry. 2010; 21(2):191-200.
12 Lee KW. Clinical neurology. Seoul: Beommunsa. 2003:203-15.
13 Byeon YC. The mapping development research of dementia management. Korea Institute for Health and Social Affairs. 1997.
14 Citron M, Diehl TS,gordong, Biere AL, Seubert P, Selkoe DJ. Evidence that the 42- and 40-amino acid forms of amyloid beta protein aregenerated from the beta-amyloid precursor protein by different protease activities. Proc. Natl. Acad. Sci. USA. 1996;93:13170-5.   DOI
15 McGrath LT. Increased oxidative stress in alzheimer's disease as assessed with 4-hydroxynonenal but not malondialdehyde. Q J medicin. 2001;94:485-90.   DOI   ScienceOn
16 David S. Treatmentguidelines for alzheimer's disease - redefinding perceptions in primary care. Primary care companion J clinic psychiatry. 2007;9(2):113-21.   DOI
17 Cheng, Chen KS, Knox J, Inglis J, Bernard A, Martin SJ, et al. A learning deficit related to age and beta-amyloid plaques in a mouse model of Alzheimer' disease. Nature.2000;408:975 9.   DOI   ScienceOn
18 Heo J. Dongeuibogam. Seoul : Dongeuibogam publisher. 2005:98-100.
19 Lee SI. Clinical applications of herbal medicine. Seoul:Seongbosa. 1990:151-3, 308-13,419-20, 426-8.
20 Kim YW, Song TW, Oh MS. Effect of Chongmyungtang on learning and memory in normal and scopolamine-induced amnestic mice. Journal of Oriental Rehabilitation Medicine. 1998;8(2):464-79.
21 Lim JH, Lee SR. The Effects of PalMihapChongMungTang(PMCMT) hot water extract & ultra-fine powder on the Alzheimer's disease model. Journal of oriental neuropsychiatry. 2008;19(2):95-110.
22 Kim HJ, Lee SR. The Effects of KakamBoyangHwanoh-Tang(KBHT) and PalMihapChongMung-Tang(PMCMT) on protecting microglia and inhibiting acetylcholinesterase and oxidants. Journal of oriental neuropsychiatry. 2008;19(2):65-75.
23 Lee SH, Jung IC, Lee SR. Effect of KwyBiChongMung-Tang hot water extract & ultra-fine powder on the Alzheimer's disease model. Korean journal of oriental physiology & pathology. 2007;21(4):921-33.
24 Choi KW, Lee SR, Jung IC. Effects of ChongMyung-Tang and ChongMyung-Tang added Hibiscus syriacus hot water extract & ultra-fine powder on microglia and memory deficit model. Journal of oriental neuropsychiatry. 2006;20(2):1200-10.
25 Sinha S, Anderson JP, Barbour R, BasigS, Caccavello R, Davis D, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature. 1999;402:537-40.   DOI   ScienceOn
26 The Korean society of oriental neuropsychiatry. Oriental neuropsychiatry. Seoul:Jipmundang. 2007:324-33.
27 Oide T, Kinoshita T, Arima K. Regression stage senile plaques in the natural course of alzheimer's disease. Neuropathology appl neurobiology. 2006;32(5):539-56.   DOI   ScienceOn