Browse > Article
http://dx.doi.org/10.7231/JON.2012.23.1.145

The Effects of Jeoreongchajeonja-tang(Zhulingjuqianzi-tang) on the βA and LPS Induced BV2 microglial cell  

Ryu, Chang-Hee (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
Jung, In-Chul (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
Lee, Sang-Ryong (Dept. of Neuropsychiatry, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Oriental Neuropsychiatry / v.23, no.1, 2012 , pp. 145-159 More about this Journal
Abstract
Objectives : This research investigates the effect of the JCT extract regarding Alzheimer's disease. Methods : The effects of the JCT extract on IL-$1{\beta}$, IL-6, TNF-${\alpha}$, COX-2, NOS-II mRNA, APP mRNA, BACE mRNA, Nitric oxide(NO), and ${\beta}A$ protein production in the BV2 microglia cell lines treated with LPS and ${\beta}A$ were investigated. Results : 1. The JCT extract suppressed the expression of IL-$1{\beta}$, IL-6, TNF-${\alpha}$, COX-2, and NOS-II mRNA in BV2 microglial cell line treated with LPS and ${\beta}A$. 2. The JCT extract suppressed the expression of BACE and APP mRNA in BV2 microglial cell line treated with LPS and ${\beta}A$. 3. The JCT extract suppressed the expression of Nitric oxide(NO) in BV2 microglial cell line treated with LPS and ${\beta}A$. 4. The JCT extract suppressed the expression of ${\beta}A$ protein production in BV2 microglial cell line treated with LPS and ${\beta}A$. Conclusions : These results suggest that the JCT group may be effective for the treatment of Alzheimer's disease. Thus, JCT could be considered among the future therapeutic drugs indicated for the treatment of Alzheimer's disease.
Keywords
alzheimer's disease; Jeoreongchajeonja-tang(Zhulingjuqianzi-tang); lipopolysacchride(LPS); ${\beta}$-amyloid(${\beta}A$);
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Griess, P. Bemerkungen zu der abhandlung der H.H. Weselsky und Benedikt "Ueber einige azoverbindungen." Chem. Ber. 1979;12:426-8.
2 Suzuki N, Cheung T, Cai X, Odaka A, Otvos L, Eckman C, Golde T, Younkin S. Science. 1994; 264,1336-40.   DOI   ScienceOn
3 Bradford M. "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" Anal. Biochem. 1976;72:248-54.   DOI   ScienceOn
4 Lee KH.. The latest clinical psychiatry. Seoul:Hana Medicine Publisher. 1998:138
5 Lee KW. Clinical neurology. Seoul:Beommunsa. 2003:203-15.
6 Min SK. The latest psychiatry Seoul:Iljogak. 2009: 679-81.
7 Department of the national association of Sasang typology. Sasang typology. Seoul: Jipmundang. 2005;492-3.
8 Chae JG, Jung IC, Lee SR. The effects of crataegus pinnatifida BGE. var. major N.E. BR(CPVM) extract on the alzheimer's disease model induced by CT105. Journal of Oriental Neuropsychiatry. 2002;13(1): 1-24.
9 Choi H, Kim SH, Lee DY, Ahn DJ, Kang HW, Lyu YS. The effects of rhizoma acori graminei water extract in alzheimer's disease model induced by pCT105. Journal of Oriental Neuropsychiatry. 2002;13(2):279 -88.
10 Kim MJ, Jung IC, Lee SR. The effects of chaenomelis fructus(CMF) extract on the alzheimer's disease model induced by CT- 105 and $\beta A$. Journal of Oriental Neuropsychiatry. 2005;16(1):97-117.
11 Kim TH, Kim JH, Kang HW, Lyu YS. Studies on inhibitory effect of inflammatory cytokines secretion from brain astrocytes by Sesim-Tang. Journal of Oriental Neuropsy- chiatry. 2001;12(1):137-50.   과학기술학회마을
12 Ha SY, Jung IC, Lee SR. Effects of Kakamgobon -Hwan(KGBH) on inhibition of impairment of learning and memory, and acetylcholinesterase in amnesia mice. Journal of Oriental Neuropsychiatry. 2002;13(1):1-22.
13 Eom HS. Effects of Gamisinsunbulo-Dan on learning and memory function in the dementia rat by ibotenic acid damage. Korean Journal of Oriental Physiology & Pathology . 2002;16(6): 1151-6.   과학기술학회마을
14 Park WS, Lee TY, Kim SY, Lee KK, Yuk SW, Lee CH, Lee SR. The immediate effect of electroacupuncture at the B62(Shinmaek) K6(Chohae) on the KKG of vascular dementia. The Journal of Korean Acupuncture & Moxibustion Society. 2001;18(2):67-78.   과학기술학회마을
15 Kim JH, Kim TH, Park JS, Lyu YS. Inhibitory effect of lemon oil on apoptosis in astrocytes. Journal of Oriental Neuropsychiatry. 2000;11(1): 37-46.   과학기술학회마을
16 Department of health and welfare. Statistical annual report of health and social. Seoul: Department of Health and Welfare. 1991: 236-9, 302-9.
17 The Korean society of oriental neuropsychiatry. Oriental neuropsychiatry. Seoul:Jipmundang. 2005: 311-6.
18 Weiner MF. The dem entias, diagnosis and management. Washington:American Psychiatric Press. 1991:77-166,227-48.
19 Lee KW, Jung HW. Clinical neurology. Seoul :Goryeo Medicine. 1997:199-210.
20 Lemere CA, Lopera F, Kosik KS, Lendon CL, Ossa J, Saido TC, Yamaguchi H, Ruiz A, Martinez A, Madrigal L, Hincapie L, Arango JC, Anthony DC, Koo EH, Goate AM, Selkoe DJ, Arango JC. The E280A presenilin 1 alzheimer mutation produces increased $\beta A$ 42 deposition and severe cerebellar pathology. Nat Med. 1996;2 (10):1146-50.   DOI   ScienceOn
21 Mcgeer EG, McGeer PL. The importance of inflammatory mechanism in alzheimer disease. Exp Gerontol. 1998;33(5):371-8.   DOI   ScienceOn
22 Zhang JB. Zhang jing yue quan shu. Seoul: Hanseongsa. 1978;610-1.
23 Bredt, DS, Hwang, PM, Snyder, SH. Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature. 1990;347:768-70.   DOI   ScienceOn
24 Linert W, Bridge MH, Huber M, Bjugstad KB, Grossman S, Arendash GW. In vitro and in vivo studies investigating possible antioxidant actions of nicotine ; relevance to parkinson's and alzheimer's diseases. Biochim Biophys Acta. 1999; 1454(2):143-52.
25 Foster NL, Petersen RC, Gracon SI, Lewis K. An enriched-population, double-blind, placebo-controlled, crossover study of tacrine and lecithin in Alzheimer's disease. Dementia. 1996;7(5):260-6.
26 Yoon JC, Lee SR, Jung IC. The effects of Hyungbangsabaek-San(Jingfangxiebai-San) on the alzheimer's disease model induced by $\beta$ A. Journal of Oriental Neuropsychiatry. 2010;21(2):171-89.
27 Yeon BK. Pharmacotherapy of dementia. Journal of the Korean Medical Association. 1999;42(10): 1010-4.
28 Chen SD. Shishi balam. Beijing:China Press of Traditional Chinese Medicine. 1991:125.
29 Qian KW. Bian zheng qi wen quan shu. Taipei: Gandhi Publisher. 1990;222-5,233-5.
30 Joo SS, Won TJ, Lee DI. Potential role of ursodeoxycholic acid in suppression of nuclear factor kappa B in microglial cell line(BV-2). Arch Pharm Res 2004;27:954-60.   DOI   ScienceOn
31 Rubin E. Essential pathology. 3rd edition. Seoul: Gunja Publisher. 2005.777-8.
32 Ping AL, Wendy H, Qing PH, Hiro M, Muzaffar S, Ashfaq S. Postischemic treatment with a calpain inhibitor MDL 28170 ameliorates brain damage in a gerbil model of global ischemia. Neurosci Lett. 1998;247(1):17-20.   DOI   ScienceOn
33 Kim WK, Jang PG, Woo MS, Han IO, Piao HZ, Lee K, Lee H, Joh TH, Kim HS: A new anti-inflammatory agent KL-1037 represses proinflammatory cytokine and inducible nitric oxide synthase(iNOS) gene expression in activated microglia. Neuropharmacology. 2004;47:243-52.   DOI   ScienceOn
34 Bocchini, V, Mazzolla, R, Barluzzi, R, Blasi, E, Sick, P, and Kettermann, H. J. Neurosci. Res. 1991;1:616-21.
35 Byeon YC. The mapping development research of dementia management. Korea Institute for Health and Social Affairs. 1997.
36 Soto C, Barnes MC, Alzarez J, Inestrosa NC. Structural determinants of the alzheimer's amyloid $\beta$-peptide. J Neurochem. 1994;63:1191-8.   DOI
37 Forloni G, Tagliavini F, Bugiani O, Salmona M. Amyloid in alzheimer's disease and prion-related encephalopathies: Studies with synthetic peptides. Prog Neurobiol. 1996;49:287-315.   DOI   ScienceOn
38 Selkoe DJ. Amyloid beta-protein and the genetics of alzheimer's disease. J Biol Chem. 1996;271: 18295-8.   DOI
39 Krul KG. "Alzheimer's disease: selections from the world market for neurotherapeutic drugs". Kalorama Information. 2002.
40 Racchi M, Johnston JA, Flood FM, Cowburn RF, Govoni S, Amyloid precursor protein metabolism in fibroblasts from individuals with one, two or three copies of the amyloid precursor protein(APP) gene. Biochem J. 1999;338:777-82.   DOI   ScienceOn
41 Breakefield XO, Geller AI. Gene transfer into the nervous system. Mol Neurobiol. 1987;1(4):339-71.   DOI   ScienceOn
42 Lee CH, Kim TH, Lee SR, Yook TH. Neuroanatomical studies on acupoints(SJ5, PE6, SP6 and GB39) projecting to the brain area related to dimentia using neural tracer, pseudorabies virus in mouse. The Journal of Korean Acupuncture & Moxibustion Society. 2003;20(6): 168-81.
43 Mattson MP, Guo ZH, Geiger JD. Secreted form of amyloid precursor against basal glucose and glutamate transport and protcts in synaptosomes by a cyclic GMP-mediated mechanism. J Neurochem. 1999;73(2):532-7.   DOI   ScienceOn
44 Ho PI, Collins SC, Dhitavat S, Ortiz D, Ashline D, Rogers E, Shea TB. Homocysteine potentiates beta-amyloid neurotoxicity ; role of oxidative stress. J Neurochem, 2001;78(2):249-53.   DOI   ScienceOn
45 Racchi M, Johnston JA, Flood FM, Cowburn RF, Govoni S. Amyloid precursor protein metabolism in fibroblasts from individuals with one, two or three copies of the amyloid precursor protein(APP) gene. Biochem J. 1999;338:777-82.   DOI   ScienceOn
46 Lee JM. Dong ui su se bo wen. Seoul:Haengrim Publisher. 1986:102.
47 Blasi E, Barluzzi R, Bocchini V, Mazzolla R, Bistoni F. Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J Neuroimmunol. 1990;27:229-37.   DOI   ScienceOn
48 Bocchini V, Mazzolla R, Barluzzi R, Blasi E, Sick P, Kettenmann H. An immortalized cell line expresses properties of activated microglial cells. J Neurosci Res. 1992;31:616-21.   DOI   ScienceOn
49 Louise B, Asha N, Barbara C, Greer M. Murphy, Jr. $\beta$-Amyloid peptide secretion by a microglial cell line is induced by $\beta$-Amyloid(25-35) and lipopolysaccharide. The Journal of Biological Chemistry. 1996:271,16084-9.   DOI   ScienceOn
50 Gertsch J, Guttinger M, Sticher O, Heilmann J. "Relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR(RT-rt-PCR): new possibilities for the screening of anti-inflammatory and cytotoxic compounds". Pharm Res. 2002;19: 1236-43.   DOI   ScienceOn